Your Good Partner in Biology Research

  1. Home
  2. 抗体
  3. 标签内参抗体
  4. HistoneH3 Monoclonal Antibody

HistoneH3 Monoclonal Antibody

  • 货号：
    CSB-MA010418A0m
  • 规格：
    ￥400
  • 图片：
    • Western Blot
      Positive WB detected in: 10ug Hela whole cell lysate, HistoneH3 antibody at 1:10000, 1:20000, 1:40000, 1:80000, 1:160000, 1:320000, 1:640000, 1:1280000
      Secondary
      Goat polyclonal to mouse IgG at 1/50000 dilution
      Predicted band size: 15-25 KDa
      Observed band size: 15-25 KDa
      Exposure time：5s
    • Western Blot
      Positive WB detected in: Raw264.7 whole cell lysate, Raji whole cell lysate, MCF-7 whole cell lysate
      All lanes: HistoneH3 antibody at 1:20000
      Secondary
      Goat polyclonal to mouse IgG at 1/50000 dilution
      Predicted band size: 15-25 KDa
      Observed band size: 15-25 KDa
      Exposure time: 1min
    • Western Blot
      Positive WB detected in: Rabbit stomach tissue
      All lanes: HistoneH3 antibody at 1:20000
      Secondary
      Goat polyclonal to mouse IgG at 1/50000 dilution
      Predicted band size: 15-25 KDa
      Observed band size: 15-25 KDa
      Exposure time: 1min
    • Western Blot
      Positive WB detected in: Rabbit heart tissue, Rabbit liver tissue,Rabbit spleen tissue, Rabbit lung tissue, Rabbit kidney tissue, Rabbit small intestine tissue
      All lanes: HistoneH3 antibody at 1:20000
      Secondary
      Goat polyclonal to mouse IgG at 1/50000 dilution
      Predicted band size: 15-25 KDa
      Observed band size: 15-25 KDa
      Exposure time: 2min
    • Western Blot
      Positive WB detected in: MCF-7 whole cell lysate, Mouse heart tissue, Mouse liver tissue, Mouse lung tissue, Mouse brain tissue tissue
      All lanes: HistoneH3 antibody at 1:5000
      Secondary
      Goat polyclonal to mouse IgG at 1/50000 dilution
      Predicted band size: 15-25 KDa
      Observed band size: 15-25 KDa
      Exposure time: 5s
    • Western Blot
      Positive WB detected in: Hela whole cell lysate, HepG2 whole cell lysate, 293 whole cell lysate, K562 whole cell lysate, U87 whole cell lysate
      All lanes: HistoneH3 antibody at 1:20000
      Secondary
      Goat polyclonal to mouse IgG at 1/50000 dilution
      Predicted band size: 15-25 KDa
      Observed band size: 15-25 KDa
      Exposure time: 2min
    • Western Blot
      Positive WB detected in: Rat heart tissue, Rat brain tissue, Rat lung tissue, Rat stomach tissue, Rabbit small intestine tissue, Rabbit heart tissue, Rabbit liver tissue
      All lanes: HistoneH3 antibody at 1:5000
      Secondary
      Goat polyclonal to mouse IgG at 1/50000 dilution
      Predicted band size: 15-25 KDa
      Observed band size: 15-25 KDa
      Exposure time: 1min
    • Western Blot
      Positive WB detected in: Rat liver tissue, Rat spleen tissue, Rabbit spleen tissue, Rabbit lung tissue, Rabbit kidney tissue
      All lanes: HistoneH3 antibody at 1:5000
      Secondary
      Goat polyclonal to mouse IgG at 1/50000 dilution
      Predicted band size: 15-25 KDa
      Observed band size: 15-25 KDa
      Exposure time: 5s
    • Western Blot
      Positive WB detected in: Rat liver tissue, Rat brain tissue, Rat stomach tissue, Rat kidney tissue
      All lanes: HistoneH3 antibody at 1:5000
      Secondary
      Goat polyclonal to mouse IgG at 1/50000 dilution
      Predicted band size: 15-25 KDa
      Observed band size: 15-25 KDa
    • Western Blot
      Positive WB detected in: Mouse stomach tissue, Mouse heart tissue, Mouse liver tissue, Mouse brain tissue
      All lanes: HistoneH3 antibody at 1:20000
      Secondary
      Goat polyclonal to mouse IgG at 1/50000 dilution
      Predicted band size: 15-25 KDa
      Observed band size: 15-25 KDa
      Exposure time: 2min
    • Immunoprecipitating HistoneH3 in K562 whole cell lysate
      Lane 1: Mouse control IgG2b instead of CSB-MA010418A0m in Hela whole cell lysate.
      Lane 2: CSB-MA010418A0m (2μg) + Hela whole cell lysate (500μg)
      Lane 3: Hela whole cell lysate (20μg)
      For western blotting, the blot was detected with CSB-MA010418A0m at 1:10000, and a HRP-conjugated Protein G antibody was used as the secondary antibody at 1:2000
    • Immunoprecipitating HistoneH3 in Hela whole cell lysate
      Lane 1: Mouse control IgG2b instead of CSB-MA010418A0m in Hela whole cell lysate.
      Lane 2: CSB-MA010418A0m (2μg) + Hela whole cell lysate (500μg)
      Lane 3: Hela whole cell lysate (20μg)
      For western blotting, the blot was detected with CSB-MA010418A0m at 1:10000, and a HRP-conjugated Protein G antibody was used as the secondary antibody at 1:2000
    • IHC image of CSB-MA010418A0m diluted at 1:200 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
    • IHC image of CSB-MA010418A0m diluted at 1:200 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
    • Western Blott
      Positive WB detected in: Hela whole cell lysate at20μg, 10μg, 5μg, 2.5μg, 1.25μg, 0.625μg
      All lanes: HistoneH3 antibody at 1:5000
      Secondary
      Goat polyclonal to mouse IgG at 1/50000 dilution
      Predicted band size: 15-25 KDa
      Observed band size: 15-25 KDa
      Exposure time: 5s
  • 其他：

产品详情

  • 产品描述：

    This Histone H3 monoclonal antibody production began with immunization of a mouse with KDIQLARRIRGERA synthetic peptide conjugate to KLH. Several weeks after immunization, spleen cells were harvested. B cells in the spleen produced the Histone H3 antibody but could not grow in culture. The spleen cells were fused with cells of a myeloma line. The myeloma cells could not produce antibodies, but they would immortally grow in culture. The two cells were fused together to produce a hybrid cell that could be used to make the desired Histone H3 antibody. This Histone H3 monoclonal antibody was purified by Protein A and validated in ELISA, WB, IHC, IP.

    Histones are a group of basic proteins with an isoelectric point greater than 10.0. All five histones contain a large number of positively charged basic amino acids lysine and arginine, which can interact with negatively charged phosphate groups in DNA. . Among them, H3 is rich in arginine. H2A, H2B, H3, and H4 are all composed of a spherical part and a tail part.
    The number of repetitions of histone genes in various organisms is not the same, but they are all within the range of moderate repetition. Generally, the copy number of each histone gene in the same organism is the same. The arrangement of histone genes in the genome is different in different organisms. There is no certain arrangement of histone genes. In a genome with a high copy number, most of histone genes repeat in tandem to form gene clusters. The existence of a large number of repetitive sequences in the genome to encode histones is of great significance.

  • Uniprot No.：
    P68431
  • 基因名：
  • 别名：
    "Histone H3.1, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/h, Histone H3/i, Histone H3/j, Histone H3/k, Histone H3/l, H3C1, H3FA, HIST1H3A AND, H3C2, H3FL, HIST1H3B AND, H3C3, H3FC HIST1H3C AND, H3C4, H3FB, HIST1H3D AND, H3C6, H3FD, HIST1H3E AND, H3C7, H3FI, HIST1H3F AND, H3C8, H3FH, HIST1H3G AND, H3C10, H3FK, HIST1H3H AND, H3C11, H3FF, HIST1H3I AND, H3C12, H3FJ, HIST1H3J"
  • 宿主：
    Mouse
  • 反应种属：
    Human, Rat, Rabbit, Mouse
  • 免疫原：
    KDIQLARRIRGERA synthetic peptide conjugate to KLH
  • 标记方式：
    Non-conjugated
  • 克隆类型：
    Monoclonal Antibody
  • 抗体亚型：
    IgG2b
  • 纯化方式：
    >95%, Protein A purified
  • 克隆号：
    1G2E4
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
  • 产品提供形式：
    Liquid
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
  • 基因功能参考文献：
    1. Data indicate the mechanism for epigenetic regulation in cancer by inducing E3 ubiquitin ligase NEDD4-dependent histone H3 ubiquitination. PMID: 28300060
    2. the identification of increased expression of H3K27me3 during a patient's clinical course can be helpful for determining whether the tumors are heterochronous PMID: 29482987
    3. Here, we report that JMJD5, a Jumonji C (JmjC) domain-containing protein, is a Cathepsin L-type protease that mediates histone H3 N-tail proteolytic cleavage under stress conditions that cause a DNA damage response. PMID: 28982940
    4. Data suggest that Ki-67 antigen proliferative index has important limitations and hhosphohistone H3 (PHH3) is an alternative proliferative marker. PMID: 29040195
    5. These results identify cytokine-induced histone 3 lysine 27 trimethylation as a mechanism that stabilizes gene silencing in macrophages PMID: 27653678
    6. This data indicates that, in the early developing human brain, HIST1H3B constitutes the largest proportion of H3.1 transcripts among H3.1 isoforms. PMID: 27251074
    7. This series of 47 diffuse midline gliomas, histone H3-K27M mutation was mutually exclusive with IDH1-R132H mutation and EGFR amplification, rarely co-occurred with BRAF-V600E mutation, and was commonly associated with p53 overexpression, ATRX loss, and monosomy 10. Among these K27M+ diffuse midline gliomas. PMID: 26517431
    8. Data show that histone chaperone HIRA co-localizes with viral genomes, binds to incoming viral and deposits histone H3.3 onto these. PMID: 28981850
    9. These experiments showed that PHF13 binds specifically to DNA and to two types of histone H3 methyl tags (lysine 4-tri-methyl or lysine 4-di-methyl) where it functions as a transcriptional co-regulator. PMID: 27223324
    10. Hemi-methylated CpGs DNA recognition activates UHRF1 ubiquitylation towards multiple lysines on the H3 tail adjacent to the UHRF1 histone-binding site. PMID: 27595565
    11. We describe, for the first time, the MR imaging features of pediatric diffuse midline gliomas with histone H3 K27M mutation PMID: 28183840
    12. Approximately 30% of pediatric high grade gliomas (pedHGG) including GBM and DIPG harbor a lysine 27 mutation (K27M) in histone 3.3 (H3.3) which is correlated with poor outcome and was shown to influence EZH2 function. PMID: 27135271
    13. H3F3A K27M mutation in adult cerebellar HGG is not rare. PMID: 28547652
    14. Data show that lysyl oxidase-like 2 (LOXL2) is a histone modifier enzyme that removes trimethylated lysine 4 (K4) in histone H3 (H3K4me3) through an amino-oxidase reaction. PMID: 27735137
    15. Histone H3 lysine 9 (H3K9) acetylation was most prevalent when the Dbf4 transcription level was highest whereas the H3K9me3 level was greatest during and just after replication. PMID: 27341472
    16. SPOP-containing complex regulates SETD2 stability and H3K36me3-coupled alternative splicing. PMID: 27614073
    17. Data suggest that binding of helical tail of histone 3 (H3) with PHD ('plant homeodomain') fingers of BAZ2A or BAZ2B (bromodomain adjacent to zinc finger domain 2A or 2B) requires molecular recognition of secondary structure motifs within H3 tail and could represent an additional layer of regulation in epigenetic processes. PMID: 28341809
    18. The results demonstrate a novel mechanism by which Kdm4d regulates DNA replication by reducing the H3K9me3 level to facilitate formation of preinitiation complex. PMID: 27679476
    19. Histone H3 modifications caused by traffic-derived airborne particulate matter exposures in leukocytes PMID: 27918982
    20. A key role of persistent histone H3 serine 10 or serine 28 phosphorylation in chemical carcinogenesis through regulating gene transcription of DNA damage response genes PMID: 27996159
    21. hTERT promoter mutations are frequent in medulloblastoma and are associated with older patients, prone to recurrence and located in the right cerebellar hemisphere. On the other hand, histone 3 mutations do not seem to be present in medulloblastoma. PMID: 27694758
    22. AS1eRNA-driven DNA looping and activating histone modifications promote the expression of DHRS4-AS1 to economically control the DHRS4 gene cluster. PMID: 26864944
    23. Data suggest that nuclear antigen Sp100C is a multifaceted histone H3 methylation and phosphorylation sensor. PMID: 27129259
    24. The s propose that histone H3 threonine 118 phosphorylation via Aurora-A alters the chromatin structure during specific phases of mitosis to promote timely condensin I and cohesin disassociation, which is essential for effective chromosome segregation. PMID: 26878753
    25. Hemi-methylated DNA opens a closed conformation of UHRF1 to facilitate its H3 histone recognition. PMID: 27045799
    26. Functional importance of H3K9me3 in hypoxia, apoptosis and repression of APAK. PMID: 25961932
    27. Taken together, the s verified that histone H3 is a real substrate for GzmA in vivo in the Raji cells treated by staurosporin. PMID: 26032366
    28. We conclude that circulating H3 levels correlate with mortality in sepsis patients and inversely correlate with antithrombin levels and platelet counts. PMID: 26232351
    29. Data show that double mutations on the residues in the interface (L325A/D328A) decreases the histone H3 H3K4me2/3 demethylation activity of lysine (K)-specific demethylase 5B (KDM5B). PMID: 24952722
    30. Data indicate that minichromosome maintenance protein 2 (MCM2) binding is not required for incorporation of histone H3.1-H4 into chromatin but is important for stability of H3.1-H4. PMID: 26167883
    31. Data suggest that histone H3 lysine methylation (H3K4me3) serves a crucial mechanistic role in leukemia stem cell (LSC) maintenance PMID: 26190263
    32. PIP5K1A modulates ribosomal RNA gene silencing through its interaction with histone H3 lysine 9 trimethylation and heterochromatin protein HP1-alpha. PMID: 26157143
    33. Data indicate that the lower-resolution mass spectrometry instruments can be utilized for histone post-translational modifications (PTMs) analysis. PMID: 25325711
    34. Data indicate that inhibition of lysine-specific demethylase 1 activity prevented IL-1beta-induced histone H3 lysine 9 (H3K9) demethylation at microsomal prostaglandin E synthase 1 (mPGES-1) promoter. PMID: 24886859
    35. The s report that de novo CENP-A assembly and kinetochore formation on human centromeric alphoid DNA arrays is regulated by a histone H3K9 acetyl/methyl balance. PMID: 22473132

    显示更多

    收起更多

  • 相关疾病：
    Glioma (GLM)
  • 亚细胞定位：
    Nucleus. Chromosome.
  • 蛋白家族：
    Histone H3 family
  • 数据库链接：

    HGNC: 4766

    OMIM: 137800

    KEGG: hsa:8350

    STRING: 9606.ENSP00000444823

    UniGene: Hs.132854