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  4. Recombinant Drosophila melanogaster Potassium voltage-gated channel protein Shaker (Sh)

Recombinant Drosophila melanogaster Potassium voltage-gated channel protein Shaker (Sh)

  • 中文名称：
    黑腹果蝇Sh重组蛋白
  • 货号：
    CSB-CF357413DLU
  • 规格：
  • 来源：
    in vitro E.coli expression system
  • 其他：

产品详情

  • 基因名：
    Sh
  • Uniprot No.：
  • 别名：
    Sh; mns; CG12348; Potassium voltage-gated channel protein Shaker; Protein minisleep
  • 种属：
    Drosophila melanogaster (Fruit fly)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-655
  • 氨基酸序列
    MAAVAGLYGLGEDRQHRKKQQQQQQHQKEQLEQKEEQKKIAERKLQLREQQLQRNSLDGY GSLPKLSSQDEEGGAGHGFGGGPQHFEPIPHDHDFCERVVINVSGLRFETQLRTLNQFPD TLLGDPARRLRYFDPLRNEYFFDRSRPSFDAILYYYQSGGRLRRPVNVPLDVFSEEIKFY ELGDQAINKFREDEGFIKEEERPLPDNEKQRKVWLLFEYPESSQAARVVAIISVFVILLS IVIFCLETLPEFKHYKVFNTTTNGTKIEEDEVPDITDPFFLIETLCIIWFTFELTVRFLA CPNKLNFCRDVMNVIDIIAIIPYFITLATVVAEEEDTLNLPKAPVSPQDKSSNQAMSLAI LRVIRLVRVFRIFKLSRHSKGLQILGRTLKASMRELGLLIFFLFIGVVLFSSAVYFAEAG SENSFFKSIPDAFWWAVVTMTTVGYGDMTPVGVWGKIVGSLCAIAGVLTIALPVPVIVSN FNYFYHRETDQEEMQSQNFNHVTSCPYLPGTLGQHMKKSSLSESSSDMMDLDDGVESTPG LTETHPGRSAVAPFLGAQQQQQQPVASSLSMSIDKQLQHPLQQLTQTQLYQQQQQQQQQQ QNGFKQQQQQTQQQLQQQQSHTINASAAAATSGSGSSGLTMRHNNALAVSIETDV
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal 10xHis-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    Lyophilized from Tris/PBS-based buffer, 6% Trehalose, pH 8.0
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Voltage-gated potassium channel that mediates transmembrane potassium transport in excitable membranes. The channel alternates between opened and closed conformations in response to the voltage difference across the membrane. Forms rapidly inactivating tetrameric potassium-selective channels through which potassium ions pass in accordance with their electrochemical gradient and may contribute to A-type currents. Plays a role in the regulation of sleep need or efficiency. Plays a role in sexual behavior, where it is imortant for male sex discrimination.
  • 基因功能参考文献：
    1. S4-S5 linker movement during activation and inactivation in voltage-gated K(+) channels. PMID: 29959207
    2. s provide evidence that in the Drosophila Shaker voltage-gated K(+) channels the S3 domain acts as an extracellular hydrophobic 'stabilizer' for the S4 domain biasing the gating chemical equilibrium toward the open state. PMID: 23542156
    3. This study demonstrates that Shaker is not always a good model for Kv1 channels for ligand binding. PMID: 23398369
    4. The xenon requirement in Drosophila melanogaster is not influenced by a single gene mutation at the shaker locus, whereas a reduced expression of a nonselective cation channel leads to an increased xenon requirement. PMID: 22761550
    5. Data show several strong Cysteine-Cadmium-Cysteine bridges in Shaker potassium channel. PMID: 22538811
    6. Synaptic transmission at the larval neuromuscular junction and the transient Shaker potassium current IA in larval muscles have been characterized. PMID: 20429677
    7. interaction of the voltage-dependent Shaker potassium channel with the channel-blocking toxin kappa-conotoxin-PVIIA PMID: 11820396
    8. Shaker K+ channels contribute to the information capacity of Drosophila photoreceptors PMID: 12571596
    9. Second-order kernels of Shaker potassium channel mutants lack a large, early amplification and demonstrate a novel role for Shaker K+ channels in amplifying and accelerating the voltage response of wild-type photoreceptors. PMID: 12761281
    10. Results suggest that the external TEA binding site is not within the membrane electric field and that the voltage dependence of TEA block in K+ solutions arises through a coupling with the movement of K+ ions through part of the membrane electric field. PMID: 12885878
    11. reduce the metabolic cost of neural information in Drosophila photoreceptors. PMID: 12952637
    12. The Shaker voltage-gated potassium channel pore opens when the inner helix bends at a conserved glycine gating hinge. PMID: 15491131
    13. Shaker, which encodes a voltage-dependent potassium channel controlling membrane repolarization and transmitter release, may thus regulate sleep need or efficiency; short-sleeping Shaker flies have a reduced lifespan PMID: 15858564
    14. observations highlight crucial control of nerve terminal excitability by Shaker & Shab channels to confer temporal patterns of synaptic transmission & suggest participation of these channels in activity-dependent synaptic plasticity PMID: 16763031
    15. ShB(delta)K wes activated by binding with toxin 6-bromo-2-mercaptotryptamine (BrMT). PMID: 16801385
    16. These results suggest that cooperative insertion of the voltage-sensor transmembrane helices is a property common to K(v) channels and that the degree of cooperativity depends on a balance between electrostatic and hydrophobic forces. PMID: 17488813
    17. synaptic strength and synaptic strength at neuromuscular junctions along the dorsal-ventral differentials at physiological Ca(2+) levels were not significantly altered in slowpoke (slo) and Shaker (Sh) mutants PMID: 18539401
    18. allosteric communication across disparate regions of the channel protein and between evolved and regulated amino acid changes introduced by RNA editing. PMID: 19114634

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  • 亚细胞定位：
    Cell membrane; Multi-pass membrane protein.
  • 蛋白家族：
    Potassium channel family, A (Shaker) (TC 1.A.1.2) subfamily, Shaker sub-subfamily
  • 数据库链接：

    KEGG: dme:Dmel_CG12348

    STRING: 7227.FBpp0088599

    UniGene: Dm.7088