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  4. Recombinant Human Aquaporin-5 (AQP5)

Recombinant Human Aquaporin-5 (AQP5)

In Stock
  • 货号：
    CSB-CF001965HU
  • 规格：
    ￥9720
  • 图片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他：

产品详情

  • 纯度：
    Greater than 85% as determined by SDS-PAGE.
  • 基因名：
    AQP5
  • Uniprot No.：
  • 别名：
    (AQP-5)
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Full Length
  • 来源：
    in vitro E.coli expression system
  • 分子量：
    31.1 kDa
  • 表达区域：
    1-265aa
  • 氨基酸序列
    MKKEVCSVAFLKAVFAEFLATLIFVFFGLGSALKWPSALPTILQIALAFGLAIGTLAQALGPVSGGHINPAITLALLVGNQISLLRAFFYVAAQLVGAIAGAGILYGVAPLNARGNLAVNALNNNTTQGQAMVVELILTFQLALCIFASTDSRRTSPVGSPALSIGLSVTLGHLVGIYFTGCSMNPARSFGPAVVMNRFSPAHWVFWVGPIVGAVLAAILYFYLLFPNSLSLSERVAIIKGTYEPDEDWEEQREERKKTMELTTR
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal 10xHis-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    3-7 business days
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Forms a water-specific channel. Plays an important role in fluid secretion in salivary glands. Required for TRPV4 activation by hypotonicity. Together with TRPV4, controls regulatory volume decrease in salivary epithelial cells. Seems to play a redundant role in water transport in the eye, lung and in sweat glands.
  • 基因功能参考文献：
    1. Results indicate that autophagy plays a crucial role in aquaporin 5 (AQP5) degradation in diabetic submandibular gland (SMG). PMID: 29951954
    2. these results suggest that AQP5 silencing enhances the sensitivity of colorectal cancer (CRC) cells to 5-FU, and the underlying mechanism is related to inhibition of the Wnt-beta-catenin pathway. AQP5 could be a useful therapeutic target for CRC treatment. PMID: 29390193
    3. Structural basis for mutations in human AQP5 associated with keratoderma has been described. PMID: 29799470
    4. The urea transporter subtypes, UT-A1 and UT-B1, were expressed in the skin basal cell layer and exocrine sweat glands. The abundance of UT-A1 and UT-B1 in uremic sweat glands was significantly increased in UP, while the expression of AQP5 was decreased. PMID: 29279852
    5. we demonstrated that AQP5 gene silencing could inhibit the proliferation, reduce the migration and promote the apoptosis of human glioma cells by suppressing EGFR/ERK/p38 MAPK signaling pathway PMID: 28404978
    6. Data suggest that urine AQP5/creatinine ratio is significantly higher in patients with diabetic nephropathy than in control subjects, subjects diabetes, or subjects with nephropathy of unknown etiology; urine AQP5/creatinine ratio increases with stage of diabetic nephropathy; this biomarker may improve clinical models in distinguishing diabetic nephropathy from normal controls and subjects with type 2 diabetic alone. PMID: 27103565
    7. We present the first Danish family diagnosed with autosomal dominant palmplantar ketratoderma of Bothnian type resulting from c.562C>T, p.Arg188Cys in the AQP5 gene. PMID: 27255181
    8. Findings show that overexpression of aquaporin 5 (AQP5) activated epithelial-mesenchymal transition (EMT) in colorectal cancer (CRC) cells. PMID: 28833571
    9. RAQP5 protein expression in breast cancer.Rac1 is a potential downstream signaling partner of AQP5 in breast cancer. PMID: 28958942
    10. The AQP5 genotype may influence survival following lipopolysaccharides by altering neutrophil cell migration. PMID: 27871297
    11. Mucins and AQP5 gene expression were significantly higher in patients with OME relative to controls. A 2-fold increase in AQP5 correlated with increased effusion viscosity (1.94 mL/mg; 95% CI, 0.08-3.80 mL/mg). PMID: 28594978
    12. The severity of preterm infants with acute respiratory distress syndrome was associated with the plasma level of AQP5; the more severe of the disease, the higher levels of plasma AQP5. PMID: 25877715
    13. AQP5 is up-regulated in hepatocellular carcinoma (HCC) cell line. AQP5 promotes hepatocellular carcinoma metastasis via NF-kappa B-regulated epithelial-mesenchymal transition. PMID: 28619511
    14. Double IF showed the co-localization of AQP5 and LC3B on BafA1-treated heated cells. In conclusion, we demonstrated that heat shock decreased AQP5 on cellular membranes and in the cytoplasm by activating autophagic degradation, and heat shock and AQP5 knockdown exerted similar anticancer effects, suggesting that heat shock exerts anticancer effects via the autophagic degradation of AQP5. PMID: 28358429
    15. we analyzed the urine excretion of AQP5 and AQP2 via exosomes, in 35 diabetic patients: 12 normoalbuminuric with normal renal function (DM), 11 with proteinuric nondiabetic nephropathy (NDN), and 12 with histological diagnosis and classification of DN. urinary AQP5 and AQP2 were significantly increased in DN patients. PMID: 28246612
    16. this study shows that S-allylmercapto-l-cysteine can suppress AQP5 secretion using the cell model of chronic obstructive pulmonary disease PMID: 27517516
    17. The current study suggests AQP5 rs1964676 as a new potential prognostic marker in patients with EBC involved in AQP5 expression PMID: 27978515
    18. Adjusting AQP5 protein levels could be considered a therapeutic strategy for the treatment of acute pulmonary edema induced by H2S and other hazardous gases PMID: 28088675
    19. nuclear matrix protein 4 overexpression increased Aquaporin 5 mRNA expression by 2.5-fold in HEK293 cells PMID: 27058007
    20. The findings presented here add further support to mutations in AQP5 being responsible for this particular subtype of NEPPK and also have implications for the optimal initial genetic screening of other British individuals with this clinical diagnosis. PMID: 26032342
    21. AQP5 plasma membrane abundance in transfected HEK293 cells is rapidly and reversibly regulated by at least three independent mechanisms involving phosphorylation at Ser156, protein kinase A activity and extracellular tonicity. PMID: 26569106
    22. AQP5 can be both overexpressed and lost in subgroups of prostate cancers PMID: 26614400
    23. AQP5 defined a subset of patients with Bcl-2-negative and p16-negative tumours with a poor clinical outcome. PMID: 26074259
    24. Histamine downregulates AQP5 production in human nasal epithelial cells by inhibiting cyclic adenosine monophosphate-responsive element binding protein (CREB) phosphorylation at serine 133. PMID: 25781725
    25. Aquaporin-5 is expressed in adipocytes with implications in adipose differentiation. PMID: 25631586
    26. These results indicate that NFAT5 plays important roles in proliferation and migration of human lung adenocarcinoma cells through regulating AQP5 expression, providing a new therapeutic option for lung adenocarcinoma therapy. PMID: 26299924
    27. The hyperosmotic induction of AQP5 and VEGF in retinal pigment epithelial cells was in part dependent on activation of NFAT5. PMID: 25878490
    28. AQP5 was strongly localized in the apical membrane and weakly localized in the cytoplasm of secretory epithelial cells. PMID: 25218052
    29. The AQP5 protein is up-regulated in prostate cancer and is closely related to advanced stage, lymph node metastasis, and poor prognosis. AQP5 expression was associated with cell proliferation and migration. PMID: 25217331
    30. In colorectal adenocarcinoma, 31.1% had high levels of expression of AQP5, 64.4% exhibited a moderate level of staining, and 4.4% had an absence of AQP5 staining. AQP5 was only occasionally detected in para-neoplastic (6.67%) and normal tissues (6.67%). PMID: 25109507
    31. Down-regulated aquaporin 5 inhibits proliferation and migration of human epithelial ovarian cancer 3AO cells. PMID: 25298246
    32. These findings indicate that AQP5-mediated regulation of microtubule dynamics modulates airway epithelial barrier properties and epithelial function. PMID: 22715407
    33. Survival of patients with a high AQP-5/PTEN coexpression was longer than that of patients with a low coexpression (p = 0.003). PMID: 24217644
    34. High AQP5 expression is associated with breast cancer. PMID: 24114055
    35. So these results indicate that AQP5 is associated with drug resistance of colon cancer, and that the AQP5-P38 MAPK pathway may represent a potential drug target to improve drug resistance of colon cancer cells. PMID: 24752576
    36. We described a Chinese family with palmoplantar keratoderma Bothnia type in which we identified a gain-of-function mutation in AQP5. PMID: 23867895
    37. By directing cellular localization of TRPC1 and AQP5 channels and by selectively regulating the functional assembly TRPC1-STIM1 channels, Cav1 is a crucial determinant of SG fluid secretion. PMID: 23203809
    38. AQP5-L51R does not exhibit the H2O or CO2 permeability of the wild-type protein. PMID: 23842530
    39. Mutations in AQP5 cause autosomal-dominant diffuse nonepidermolytic palmoplantar keratoderma. PMID: 23830519
    40. The regulated AQP5 translocation may contribute to sweat secretion by increasing the water permeability of apical plasma membranes of sweat glands. PMID: 23473857
    41. AQP5 polymorphism A(-1364)C is associated with peritumoral brain edema in meningioma patients PMID: 23392848
    42. Our findings clearly indicate that binding of anti-M3R autoantibodies to the receptor, which was verified by immunoprecipitation, suppresses AQP5 trafficking to the membrane and contribute to impaired fluid secretion in SjS. PMID: 23382834
    43. Overexpression of AQP5 is associated with lymphatic Metastasis in gastric carcinoma. PMID: 23436048
    44. These observations suggest that AQP5 plays a key role in cervical cancer PMID: 22048942
    45. These observations suggest that sialadenosis is associated with a different AQP5 expression and distribution pattern in salivary acinar cells. PMID: 23160677
    46. A lipid, phosphatidylserine, is bound to AQP5 in the central pore, totally occluding it and inhibits the function of the central pore with an IC(50) in the micromolar range. PMID: 23176748
    47. The variant G allele of AQP5 polymorphism rs3736309 reduces the risk of Meniere's disease. PMID: 23352976
    48. AQP5 is a significant component of lens fiber cell membranes, representing the second most abundant water channel in these cells. PMID: 23313152
    49. The potential functions of AQP2 and AQP5 are in the resorption and secretion of endolymph. (Review) PMID: 22732097
    50. TNF-alpha inhibition of AQP5 expression in human salivary gland acinar cells is due to the epigenetic mechanism by suppression of acetylation of histone H4. PMID: 21973049

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  • 相关疾病：
    Keratoderma, palmoplantar, Bothnian type (PPKB)
  • 亚细胞定位：
    Apical cell membrane; Multi-pass membrane protein. Cell membrane; Multi-pass membrane protein. Cytoplasmic vesicle membrane; Multi-pass membrane protein.
  • 蛋白家族：
    MIP/aquaporin (TC 1.A.8) family
  • 组织特异性：
    Detected in skin eccrine sweat glands, at the apical cell membrane and at intercellular canaliculi (at protein level).
  • 数据库链接：

    HGNC: 638

    OMIM: 600231

    KEGG: hsa:362

    STRING: 9606.ENSP00000293599

    UniGene: Hs.298023