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  4. Recombinant Human Ceramide glucosyltransferase (UGCG)

Recombinant Human Ceramide glucosyltransferase (UGCG)

In Stock
  • 货号：
    CSB-CF619088HU
  • 规格：
    ￥9720
  • 图片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他：

产品详情

  • 纯度：
    Greater than 85% as determined by SDS-PAGE.
  • 基因名：
    UGCG
  • Uniprot No.：
  • 别名：
    UGCG; Ceramide glucosyltransferase; GLCT-1; Glucosylceramide synthase; GCS; UDP-glucose ceramide glucosyltransferase; UDP-glucose:N-acylsphingosine D-glucosyltransferase
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Full Length
  • 来源：
    in vitro E.coli expression system
  • 分子量：
    47.7 kDa
  • 表达区域：
    1-394aa
  • 氨基酸序列
    MALLDLALEGMAVFGFVLFLVLWLMHFMAIIYTRLHLNKKATDKQPYSKLPGVSLLKPLKGVDPNLINNLETFFELDYPKYEVLLCVQDHDDPAIDVCKKLLGKYPNVDARLFIGGKKVGINPKINNLMPGYEVAKYDLIWICDSGIRVIPDTLTDMVNQMTEKVGLVHGLPYVADRQGFAATLEQVYFGTSHPRYYISANVTGFKCVTGMSCLMRKDVLDQAGGLIAFAQYIAEDYFMAKAIADRGWRFAMSTQVAMQNSGSYSISQFQSRMIRWTKLRINMLPATIICEPISECFVASLIIGWAAHHVFRWDIMVFFMCHCLAWFIFDYIQLRGVQGGTLCFSKLDYAVAWFIRESMTIYIFLSALWDPTISWRTGRYRLRCGGTAEEILDV
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal 10xHis-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    3-7 business days
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Catalyzes at the cytosolic surface of the Golgi, the initial step of the glucosylceramide-based glycosphingolipid/GSL synthetic pathway, the transfer of glucose from UDP-glucose to ceramide to produce glucosylceramide/GlcCer. Glucosylceramide is the core component of glycosphingolipids/GSLs, amphipathic molecules consisting of a ceramide lipid moiety embedded in the outer leaflet of the membrane, linked to one of hundreds of different externally oriented oligosaccharide structures. Glycosphingolipids are essential components of membrane microdomains that mediate membrane trafficking and signal transduction. They are implicated in many fundamental cellular processes, including growth, differentiation, migration, morphogenesis, cell-to-cell and cell-to-matrix interactions. They are required for instance in the proper development and functioning of the nervous system. As an example of their role in signal transduction, they regulate the leptin receptor/LEPR in the leptin-mediated signaling pathway. They also play an important role in the establishment of the skin barrier regulating keratinocyte differentiation and the proper assembly of the cornified envelope. The biosynthesis of GSLs is also required for the proper intestinal endocytic uptake of nutritional lipids.
  • 基因功能参考文献：
    1. Studies show a connection between UDP-glucose ceramide glucosyltransferase (UGCG) and multidrug resistance protein 1 (MDR1) overexpression and multidrug resistance development [Review]. PMID: 29409484
    2. Results suggest that the changes of DNA methylation status of the glucosylceramide synthase (GCS) promoter correlates with multidrug resistance in breast cancer. PMID: 27191984
    3. Glucosylceramide synthase upregulation is associated with sorafenib resistance in hepatocellular carcinoma. PMID: 26811497
    4. GCS was upregulated in colorectal carcinoma tissues compared to control tissues. PMID: 25535133
    5. We found upregulation of specific sphingolipid enzymes, namely sphingomyelin synthase 1 (SMS1), sphingomyelinase 3 (SMPD3), and glucosylceramide synthase (GCS) in the endometrium of endometriotic women. PMID: 24960545
    6. Our data demonstrates a correlation between the expression of the GCS protein and ER-positive/HER-2 negative breast cancer PMID: 24456584
    7. our work indicates that some UGCG polymorphisms are modifying factors in the severity of GD. PMID: 23913449
    8. GCS was upregulated in PTCs and might be an independent factor affecting prognosis. PMID: 24342307
    9. Glucosylceramide synthase mRNA were reduced by 62%. PMID: 24510559
    10. The results thus show that ARF6 regulates neuronal differentiation through an effect on glucosylceramide synthase and glucosylceramide levels. PMID: 23555901
    11. DOX could modulate the expression of GCS through the Sp1 site of GCS promoter in ERalpha-positive breast cancer cells PMID: 23133636
    12. Ceramide glycosylation catalyzed by glucosylceramide synthase is important for cancer stem cells in drug resistance and tumorigenesis. PMID: 22936806
    13. Data indicate that a high expression of glucosylceramide synthase (GCS) seemed to be an indicator of poor prognosis. PMID: 20843709
    14. The s conclude that hepatitis C virus proteins, especially NS5A and NS5B, have positive effects on the expression of human GlcT-1. PMID: 22270805
    15. GlcT-1 is up-regulated at the mRNA and protein levels during the course of U937 differentiation, resulting in increased amounts of GlcCer. PMID: 21558327
    16. Data show that nilotinib induces apoptosis through upregulating ceramide synthase genes and downregulating SK-1 in CML cells in addition to inhibition of BCR/ABL. PMID: 21756066
    17. GCS overexpression was highly associated with ER-positive and HER2-positive breast cancer with metastasis. PMID: 21617856
    18. Data show that GCS silencing increased the levels of phosphorylated p53 and p53-responsive genes. PMID: 21278235
    19. inhibition of the GCS gene affects the expression of MDR1 mRNA and P-gp function. PMID: 21380926
    20. GCSshRNA could efficiently suppress GCS and MDR1 expression in vitro and in vivo and these findings may be used as one of the methods to reverse multidrug resistance in breast cancer PMID: 19693666
    21. study demonstrates, for the first time, that GCS upregulates MDR1 expression modulating drug resistance of cancer. GSLs, in particular globo series GSLs mediate gene expression of MDR1 through cSrc and beta-catenin signaling pathway PMID: 20540746
    22. Positive correlation was detected between the expression of GCS and MDR1 mRNA in K562/A02 cells and MDR1 mRNA expression was down regulated after silencing the GCS gene expression. PMID: 20533270
    23. serve as negative regulator for ceramide PMID: 11915344
    24. Glucosylceramide synthase and its functional interaction with RTN-1C regulate chemotherapeutic-induced apoptosis in neuroepithelioma cells. PMID: 12873973
    25. inhibition of glucosylceramide synthase led to increased CER galactosylation and protected U937 and HL-60 cells from DNR-induced apoptosis PMID: 14766899
    26. glucosylceramide synthase is not solely responsible for drug resistance in cancer cells PMID: 15263008
    27. p-gp confers resistance to ceramide-induced apoptosis, with modulation of the ceramide-glucosylceramide pathway GCS making a marked contribution to this resistance PMID: 15661399
    28. 10 genes were down-regulated following treatment of the T-ALL cells with 0.15 and 1.5 microg/mL of metal ores at 72 h. Transferase activity, transferring glycol groups PMID: 15747776
    29. the reticulon family member RTN-1C has been expressed and purified in Escherichia coli and its molecular structure has been analysed by fluorescence and Circular Dichroism spectroscopy PMID: 16500619
    30. it is suggested that a high level of GCS in leukemia is possible contributed to multidrug resistance of leukemia cells. PMID: 17709137
    31. Western analysis for glucosylceramide synthase showed a significant decrease in Alzheimer disease brain consistent with the hypothesis that enzyme dysfunction contributes to neuronal decay. PMID: 18155680
    32. High glucosylceramide synthase is associated with breast cancer. PMID: 18560890

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  • 亚细胞定位：
    Golgi apparatus membrane; Multi-pass membrane protein.
  • 蛋白家族：
    Glycosyltransferase 2 family
  • 组织特异性：
    Found in all tissues examined.
  • 数据库链接：

    HGNC: 12524

    OMIM: 602874

    KEGG: hsa:7357

    STRING: 9606.ENSP00000363397

    UniGene: Hs.304249