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  4. Recombinant Mouse Metalloendopeptidase homolog PEX (Phex)

Recombinant Mouse Metalloendopeptidase homolog PEX (Phex)

  • 中文名称：
    小鼠Phex重组蛋白
  • 货号：
    CSB-CF017896MO
  • 规格：
  • 来源：
    in vitro E.coli expression system
  • 其他：

产品详情

  • 基因名：
    Phex
  • Uniprot No.：
  • 别名：
    Phex; Hyp; Pex; Phosphate-regulating neutral endopeptidase PHEX; Metalloendopeptidase homolog PEX; Phosphate regulating neutral endopeptidase; Vitamin D-resistant hypophosphatemic rickets protein; X-linked hypophosphatemia protein; HYP
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-749
  • 氨基酸序列
    MEAETGSTMETGKGTNRGIRIALALFIGGTLVLGTLLFLVSQGLLSFQAKQEYCLKPECIEAAAAIMSKVNLSVDPCENFFRFACDGWISNNPIPEDMPSYGVYPWLRHNVDLKLKALLEKSVSRRRDTEAVQKAKILYSSCMNEKAIEKADAKPLLHILRHSPFRWPVLEANIGPEGVWSERKFSLLQTLATFRGQYSNSVFIRLYVSPDDKASNEHILKLDQATLSLAVREDFLDNTTEAKSYRDALYKFMVDTAVLLGANSSRAEHDMKSVLRLEIKIAEIMIPHENRTSEAMYNKMNISELSAMIPQFDWLGYIKKVIDTRLYPHLKDIGPSENVVVRVPQYFKDLFRILGAERKKTIANYLVWRMVYSRIPNLSRRFQYRWLEFSRVIQGTTTLLPQWDKCVNFIESALPYVVGKMFVNVHFQEDKKEMMEELIEGVRWAFIDMLEKENEWMDAGTKRKAQEKARAVLAKVGYPEFIMNDTYVNEDLKAIKFSESDYFGNVLQTRKYLAQSDFFWLRKAVPKTEWFTNPTTVNAFYSASTNQIRFPAGELQKPFFWGTEYPRSLSYGAIGVIVGHEFTHGFDNNGRKYDKNGNLDPWWSVESEEKFKEKTKCMINQYSNYYWKKAGLNVKGKRTLGENIADNGGLREAFRAYRKWINDRRQGVEEPLLPGITFTNNQLFFLSYAHVRCNSYRPEAAREQVQIGAHSPPQFRVNGAISNFEEFQKAFNCPRNSTMNRGADSCRLW
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal 10xHis-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    Lyophilized from Tris/PBS-based buffer, 6% Trehalose, pH 8.0
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Peptidase that cleaves SIBLING (small integrin-binding ligand, N-linked glycoprotein)-derived ASARM peptides, thus regulating their biological activity. Cleaves ASARM peptides between Ser and Glu or Asp residues. Regulates osteogenic cell differentiation and bone mineralization through the cleavage of the MEPE-derived ASARM peptide. Promotes dentin mineralization and renal phosphate reabsorption by cleaving DMP1- and MEPE-derived ASARM peptides. Inhibits the cleavage of MEPE by CTSB/cathepsin B thus preventing MEPE degradation.
  • 基因功能参考文献：
    1. the phex mutation may create the lower set point for extracellular phosphate concentrations in a murine model of X-linked hypophosphatemia PMID: 23700148
    2. demonstrates a previously unknown impact of retinol on the cell-autonomous mineralization defect of Phex-deficient osteoblasts PMID: 23266491
    3. results identify full-length OPN as novel, physiologically relevant substrates for PHEX, suggesting accumulation of mineralization-inhibiting OPN fragments may contribute to the mineralization defect seen in the osteomalacic bone characteristic of XLH/HYP PMID: 22991293
    4. Mutation in Phex gene predisposes BALB/c-Phex(Hyp-Duk)/Y mice to otitis media. PMID: 23028440
    5. These data suggest that Phex mutations alter the responsiveness of bone cells to extracellular phosphate concentrations and may create a lower set point for "normal" phosphate levels. PMID: 22006791
    6. PHEX and DMP1 control a common pathway regulating bone mineralization and FGF23 production, the latter involving activation of the FGFR signaling in osteocytes. PMID: 21507898
    7. ASARM peptides likely play a bone PHEX-dependent role in renal phosphate regulation and FGF23 expression. PMID: 21177780
    8. Cooperative role of NF-{kappa}B and poly(ADP-ribose) polymerase 1 (PARP-1) in the TNF-induced inhibition of PHEX expression in osteoblasts. PMID: 20817730
    9. OPN ASARM inhibits mineralization by binding to hydroxyapatite in a phosphorylation-dependent manner and can be cleaved by PHEX. PMID: 19775205
    10. Preproenkephalin deletion in Hyp mice (Phex-deficient) partially rescues their bone mineralization defect. PMID: 20204609
    11. Phex protein is expressed in osteoblasts and osteocytes during the embryonic and postnatal periods PMID: 11811562
    12. Glucocorticoid regulation of the murine PHEX gene. PMID: 12110521
    13. MEPE may be involved in the pathogenesis defective mineralization due to Phex deficiency in X-linked hypophosphatemia (XLH) and the Hyp-mouse. PMID: 12220505
    14. mutagen-induced point mutation in phex causes exon skipping, x-linked hypophosphatemia, and rickets PMID: 12414538
    15. findings suggest that hypophosphatemia bone and teeth abnormalities partially correct after phosphate-regulating gene with homologies to endopeptidases on the X chromosome (PHEX) gene transfer but additional factors of PHEX expression are likely critical PMID: 12456809
    16. rickets and osteomalacia are not the only causes of decreased vertebral growth in Hyp mice and Phex protein may influence bone growth and mineralization by distinct pathways PMID: 14751570
    17. Phex may control mineralization and removal of hypertrophic chondrocytes and cartilage matrix in growth plate by regulating the synthesis and deposition of certain bone matrix proteins and proteases such as matrix metalloproteinase 9 PMID: 15050894
    18. downregulation of Phex expression by PTH in vitro is associated with inhibition of matrix mineralization, consistent with a role for Phex in bone mineralization PMID: 16084134
    19. TNF-alpha-mediated reduction in Phex protein is at least in part responsible for inhibition of osteoblast mineralization, and the described mechanism may contribute to the abnormal bone metabolism associated with IBD. PMID: 16890604
    20. loss of function is related to defect of type II sodium-dependent phosphate co-transporter in [developing] teeth PMID: 17251516
    21. A new mutation was mapped to X-chromosome between 65.4 cM and 66.6 cM, where Phex gene resides. Sequence analysis revealed a unique T-to-C transition mutation resulting in Phe-to-Ser substitution at amino acid 80 of PHEX protein. PMID: 17710565
    22. PHEX functions in bone to coordinate bone mineralization and systemic phosphate homeostasis by directly regulating the mineralization process and producing FGF23. PMID: 17848631
    23. findings suggest that loss of Phex function is related to overexpression of FGF23 in teeth, which is an intrinsic defect of Hyp mouse teeth. PMID: 17942069
    24. Phex Hyp-Duk/Y mice develop endolhympattic hydrops without evidence of endolymphatic duct obstruction PMID: 18289812
    25. PHEX rescued the inhibition of osteoblast culture mineralization by pASARM, and mass spectrometry of cleaved peptides obtained after pASARM-PHEX incubations identified pASARM as a substrate for PHEX PMID: 18597632
    26. The PHEX transgene corrects mineralization defects in 9-month-old hypophosphatemic mice. PMID: 19082853
    27. Our in vivo studies provide genetic evidence for a pathological role of increased FGF23 activities in regulating abnormal phosphate homeostasis in Hyp (PHEX-deficient) mice. PMID: 19584304

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  • 亚细胞定位：
    Cell membrane; Single-pass type II membrane protein.
  • 蛋白家族：
    Peptidase M13 family
  • 组织特异性：
    Expressed in bone, specifically in the osteoid and in osteocytes. Expressed in teeth, specifically in odontoblasts and ameloblasts. Expressed moderately by macrophages in the liver and has minimal expression in brown adipose tissue. Also expressed in supr
  • 数据库链接：

    KEGG: mmu:18675

    STRING: 10090.ENSMUSP00000078863

    UniGene: Mm.2529