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  4. Human activated coagulation factor Ⅻ,FⅫa ELISA Kit

Human activated coagulation factor Ⅻ,FⅫa ELISA Kit

  • 中文名称：
    人活化凝血因子Ⅻ(FⅫa)酶联免疫试剂盒
  • 货号：
    CSB-E09232h
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Human FⅫa ELISA Kit was designed for the quantitative measurement of Human FⅫa protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.312 ng/mL-20 ng/mL and the sensitivity is 0.078 ng/mL .

  • 缩写：
    FⅫa
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    0.312 ng/mL-20 ng/mL
  • 灵敏度：
    0.078 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Others
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human FⅫa in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:1 Average % 111  
    Range % 105-116  
    1:2 Average % 95  
    Range % 88-102  
    1:4 Average % 96  
    Range % 90-100  
    1:8 Average % 93  
    Range % 86-98  
  • 回收率：
    The recovery of human FⅫa spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 92 87-99  
    EDTA plasma (n=4) 108 103-114  
                 
                 
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average Corrected  
    20 2.967 2.999 2.983 2.855  
    10 2.402 2.546 2.474 2.346  
    5 1.661 1.601 1.631 1.503  
    2.5 0.997 0.985 0.991 0.863  
    1.25 0.616 0.575 0.596 0.468  
    0.625 0.411 0.445 0.428 0.300  
    0.312 0.258 0.250 0.254 0.126  
    0 0.132 0.123 0.128 　  
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

问答及客户评论

■ 常见问题解答
Q:

Is it possible to work with citrate-plasma instead of EDTA- or heparin-plasma?
Can you please provide exemplary data from plasma samples?

A:
Thanks for your inquiry. It is recommended to detect the EDTA plasma sample with the kit.
Generally pls detect the normal healthy human sample.