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Rat β2-microglobulin,BMG/β2-MG ELISA Kit

  • 中文名称:
    大鼠β2微球蛋白(BMG/β2-MG)酶联免疫试剂盒
  • 货号:
    CSB-E11298r
  • 规格:
    96T/48T
  • 价格:
    ¥3600/¥2500
  • 其他:

产品详情

  • 产品描述:

    The Rat β2-microglobulin (BMG/β2-MG) ELISA Kit is your go-to solution for accurate and reliable quantification of Beta-2-microglobulin in rat serum, plasma, urine, and tissue homogenates.

    Designed for researchers in the field of immunology、autoimmune diseases and renal function research, this ELISA kit provides a quantitative measurement of B2M levels with a detection range of 0.156 μg/mL-10 μg/mL and a sensitivity of 0.039 μg/mL, ensuring precise and reproducible results.

    The assay principle is based on a sandwich measurement, where the captured rat β2-microglobulin is detected using a specific antibody conjugated to horseradish peroxidase. The kit's assay time is 1-5 hours, and the sample volume required is between 50-100ul.

    The Rat β2-microglobulin (BMG/β2-MG) ELISA Kit is compatible with standard 96-well microplates and requires a detection wavelength of 450 nm. This ELISA kit has been cited in more than 3 scientific publications, demonstrating its robust performance and reliability.

  • 别名:
    B2MBeta-2-microglobulin ELISA kit
  • 缩写:
  • Uniprot No.:
  • 种属:
    Rattus norvegicus (Rat)
  • 样本类型:
    serum, plasma, urine, tissue homogenates
  • 检测范围:
    0.156 μg/mL-10 μg/mL
  • 灵敏度:
    0.039 μg/mL
  • 反应时间:
    1-5h
  • 样本体积:
    50-100ul
  • 检测波长:
    450 nm
  • 研究领域:
    Immunology
  • 测定原理:
    quantitative
  • 测定方法:
    Sandwich
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of rat BMG in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
     SampleSerum(n=4)
    1:1Average %87
    Range %82-92
    1:2Average %96
    Range %92-101
    1:4Average %100
    Range %95-105
    1:8Average %90
    Range %85-95
  • 回收率:
    The recovery of rat BMG spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9790-104
    EDTA plasma (n=4)9389-97
  • 标准曲线:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    μg/mlOD1OD2AverageCorrected
    102.645 2.678 2.662 2.515
    51.855 1.896 1.876 1.729
    2.51.256 1.263 1.260 1.113
    1.250.834 0.869 0.852 0.705
    0.6250.487 0.489 0.488 0.341
    0.3120.325 0.353 0.339 0.192
    0.1560.261 0.284 0.273 0.126
    00.145 0.149 0.147  
  • 数据处理:
  • 货期:
    3-5 working days

产品评价

靶点详情

  • 功能:
    Component of the class I major histocompatibility complex (MHC). Involved in the presentation of peptide antigens to the immune system.
  • 亚细胞定位:
    Secreted.
  • 蛋白家族:
    Beta-2-microglobulin family
  • 数据库链接: