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中文名称:大鼠乙酰胆碱酯酶(AChE)酶联免疫试剂盒
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货号:CSB-E11304r
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规格:96T/48T
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价格:¥3600/¥2500
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其他:
产品详情
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产品描述:
This Rat ACHE ELISA Kit was designed for the quantitative measurement of Rat ACHE protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 7.8 pg/mL-500 pg/mL and the sensitivity is 1.95 pg/mL .
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别名:AcheAcetylcholinesterase ELISA kit; AChE ELISA kit; EC 3.1.1.7 ELISA kit
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缩写:ACHE
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Uniprot No.:
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种属:Rattus norvegicus (Rat)
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样本类型:serum, plasma, tissue homogenates
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检测范围:7.8 pg/mL-500 pg/mL
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灵敏度:1.95 pg/mL
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反应时间:1-5h
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样本体积:50-100ul
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检测波长:450 nm
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研究领域:Neuroscience
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测定原理:quantitative
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测定方法:Sandwich
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精密度:
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. -
线性度:
To assess the linearity of the assay, samples were spiked with high concentrations of rat AChE in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:100 Average % 92 Range % 88-95 1:200 Average % 96 Range % 90-101 1:400 Average % 94 Range % 90-97 1:800 Average % 99 Range % 95-103 -
回收率:
The recovery of rat AChE spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 95 91-99 EDTA plasma (n=4) 92 87-96 -
标准曲线:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. pg/ml OD1 OD2 Average Corrected 500 2.361 2.384 2.373 2.232 250 1.636 1.658 1.647 1.506 125 1.044 1.057 1.051 0.910 62.5 0.610 0.599 0.605 0.464 31.2 0.413 0.404 0.409 0.268 15.6 0.305 0.297 0.301 0.160 7.8 0.237 0.228 0.233 0.092 0 0.142 0.140 0.141 -
数据处理:
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货期:3-5 working days
引用文献
- Investigating the Short-Term Effect of Diabetic Peripheral Neuropathy on Different Neuronal Sub-classes in Circumvallate Papilla in Rats S Mostafa,/,2023
- Natural inhibitors for acetylcholinesterase and autophagy modulators as effective antagonists for tau and β-amyloid in Alzheimer's rat model M Hassan,Biomarkers,2023
- Esculetin and Fucoidan Attenuate Autophagy and Apoptosis Induced by Zinc Oxide Nanoparticles through Modulating Reactive Astrocyte and Proinflammatory Cytokines in the Rat Brain WJ Song,Toxics,2023
- Neuroprotective potential of Erigeron bonariensis ethanolic extract against ovariectomized/D-galactose-induced memory impairments in female rats in relation to its metabolite fingerprint as revealed using UPLC/MS K Himaja,Inflammopharmacology,2024
- Neurobehavioral Responses and Toxic Brain Reactions of Juvenile Rats Exposed to Iprodione and Chlorpyrifos, Alone and in a Mixture YM Abd-Elhakim,Toxics,2023
- Olive Leaf Extract Attenuates Chlorpyrifos-Induced Neuro-and Reproductive Toxicity in Male Albino Rats AA Hassan,Life,2022
- Study on the Potential Mechanism of Semen Strychni against Myasthenia Gravis Based on Network Pharmacology and Molecular Docking with Experimental Verification P Fang,Evidence-Based Complementary and Alternative Medicine,2022
- Anti-aging trait of whey protein against brain damage of senile rats ME El-Beeh,Anti-aging trait of whey protein against brain damage of senile rats,2022
- Deltamethrin and Its Nanoformulations Induce Behavioral Alteration and Toxicity in Rat Brain through Oxidative Stress and JAK2/STAT3 Signaling Pathway AG Khalifa,Toxics,2022
- Mechanism and Changes Associated with Edible Camphor Induced Neurotoxicity AA Njan - Pharmacology and Toxicology of Natural Medicines …,Pharmacol Toxicol Nat Med,2022
- Potential Toxic Effects of Exposure to Titanium Silicon Oxide Nanoparticles in Male Rats S Ghareeb,Toxicology in vitro,2022
- Evaluation of Glucosamine Effect Against Heart and Brain Damage Induced by Y-radiation or Aluminium Chloride in Female Rats AMM Hawas,The Scientific Electronic Library Online,2020
- Di(2-ethylhexyl) adipate plasticizer triggers hepatic, brain, and cardiac injury in rats: Mitigating effect of Peganum harmala oil A Behairy,Ecotoxicology and Environmental Safety,2020
- Amelioration of fenitrothion induced oxidative DNA damage and inactivation of caspase-3 in the brain and spleen tissues of male rats by N-acetylcysteine Rasha T. Alam,Life Sciences,2019
- Neuroprotective Effects of Zafirlukast, Piracetam and Their Combination on L-Methionine-Induced Vascular Dementia in Rats Fayez AM, et al,fundamental & clinical pharmacology,2019
- The subchronic exposure to malathion, an organophosphate pesticide, causes lipid peroxidation, oxidative stress, and tissue damage in rats: the protective role of resveratrol Erten Akbel.et al,Toxicol. Res.,2018
- Qualification and Verification of Serological Biomarker Candidates for Lung Adenocarcinoma by Targeted Mass Spectrometry Wu HY. et al,J Proteome Res.,2015
相关产品
靶点详情
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功能:Terminates signal transduction at the neuromuscular junction by rapid hydrolysis of the acetylcholine released into the synaptic cleft.
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基因功能参考文献:
- sepsis decreased AChE activity by reducing its expression at the neuromuscular junction. PMID: 28849127
- Paraquat-induced death of hippocampal neurons was partially mediated by AChE variants inducing and cholinergic and gultamatergic transmission disruption. PMID: 28916328
- Quercetin improves memory and anxiogenic-like behavior in diabetic rats by preventing the increase in Ache activity seen in streptozocin induced diabetes. PMID: 27694000
- the induction of PRiMA-linked AChE in osteoblast should be independent to classical estrogen signaling pathway. PMID: 27019979
- This study showed that changes in AChE may, at least in part, to lead to memory impairment caused by galactose. Taken together, our results can help understand the etiopathology of classical galactosemia. PMID: 26948151
- Acetylcholinesterase activity was increased in rats subjected to mild hyperhomocysteinemia PMID: 24590316
- Hindlimb unloading (simulation of hypogravity) leads to an increase in functional activity of acetylcholinesterase on the background of reduced postsynaptic membrane area occupied by acetylcholine receptors. PMID: 25711085
- Under all studied experimental conditions, offspring brain acetylcholinesterase (AChE) activity was found to be significantly decreased due to maternal hypothyroidism PMID: 24632022
- AChE has an important role in modulation of early immune responses against Toxoplasma gondii infection. PMID: 24039284
- Spindle-shaped AChE-rich cells in the floccular stalk project to the flocculus, nodulus and uvula, but not to the cerebellar hemispheres. PMID: 24010172
- Tyrosine increased acetylcholinesterase in blood & all tested brain areas. Acute administration decreased its mRNA levels in hippocampus. Chronic administration did the same in the hippocampus. PMID: 23046746
- Acetylcholinesterase inhibitor huperazine A improved, or partly reversed, the Abeta-induced damage of neurite outgrowth. PMID: 23119107
- in the absence of pituitary growth hormone the postnatal proliferation of cholinergic synapses in the rat spinal cord where AChE activity is abundant, is markedly reduced PMID: 22922167
- Hypothyroidism significantly reduced both specific and total AChE activity in spinal cord of rat pups PMID: 22982053
- The results from the present study demonstrate a marked increase in AChE activity in all brain structures following the administration of a branched-chain amino acid pool. PMID: 22328136
- This study demonistrated that AChE positive neurons were stained lightly, then during later developmental periods the staining gradually increased and achieved adult level of intensity on the day P21 in spinal cord. PMID: 22028090
- During induction of the non-alcoholic fatty liver disease with CCl4 AChE activity was significantly increased compared to control in blood, liver and brain tissues. PMID: 21881966
- AChE activity can act as purinergic and cholinergic markers in lymphocytes PMID: 20832780
- effect of melatonin on neuroinflammation and acetylcholinesterase activity induced by LPS in rat brain PMID: 20450904
- These findings suggest that the restricted localization of PRiMA-linked G4 AChE at the nmjs could be contributed by the pre-synaptic motor neuron and/or the post-synaptic muscle fiber. PMID: 19680821
- the importance of post-transcriptional mechanisms in regulating AChE expression in differentiating neurons and implicate HuD as a key trans-acting factor in these events PMID: 12468554
- The activity of this protein is completely suppressed by the administration of LD50 of phosphacol. PMID: 12629810
- AChE is necessary for normal dendrite and axon formation in hippocampal neurones and suggest that it may also play a role in excitatory synapse development, plasticity, and remodelling. PMID: 12799140
- Local increment in neuronal AChE concentration in the mammalian hippocampus, such as in amyloid deposits, may play a role in triggering neuropathological and behavioral changes such as those observed in Alzheimer disease brains PMID: 12969266
- In this study we show an effect of AChE that could contribute to the increased deposition of Amyloid beta PMID: 14678761
- AChE is involved in regulating cell-matrix interactions in bone PMID: 15454088
- Nearly all AChE-immunoreactive neurons in the Lateral hypothalamus express immunoreactivity for either melanin concentrating hormone or for orexin PMID: 15488307
- rats subjected to ovariectomy presented a significant increase in brain Na+, K+-ATPase, AChE and CAT activities, but did not change the oxidative stress parameters PMID: 15869839
- Results suggest that the presence of acetylcholinesterase protein doesn't correlate with catalytic activity, and the diffuse cytoplasmic and plasma membrane localization of AChE is a property of neuronal cell types. PMID: 16052514
- action of P2Y1 receptor could lead Ache gene activation in cultured cortical neurons PMID: 16429571
- Lead acetate decreased brain AChE activity of rats in dose/age dependent manner. PMID: 16678265
- The SH-amino acids seem to affect the hippocampal enzyme indirectly, possibly by lipid(s)-protein modifications(s) and Hci by inducing oxidative stress, since no effect was observed on pure AChE activity PMID: 16773467
- Results suggest that interferon-beta may act through inhibition of acetylcholinesterase, and ethidium bromide could be considered an inhibitor of AChE activity by interfering with cholinergic neurotransmission in the different brain regions. PMID: 16871442
- In the cerebellum, hyperthyroidism provoked a significant decrease in both the AChE and the Na(+), K(+)-ATPase activities PMID: 17165152
- findings provide evidence for intra- and supramodal influences of the neonatal removal of retinal input on neural activity- and use-dependent modifications of cortical AChE activity PMID: 17280650
- In choline-deprived (CD) newborn rats,ANTIOXIDANT STATUS and AChE and Na(+),K(+)-ATPase activities were significantly reduced by 23%, 24% and 50%, respectively, in the brains of both sexes. PMID: 17484629
- Low concentrations of aspartame metabolites had no effect on AChE activity, whereas high or toxic concentrations decreased enzyme activity. PMID: 17580119
- that the 55-kDa AChE may be involved in different biological processes such as neural development, tumor progression, and angiogenesis. PMID: 17606622
- These findings suggest that cell surface AChE, possibly in a novel signalling complex containing APP and perlecan, contributes to a generalised mechanism for polarised membrane protrusion and migration in all adherent cells. PMID: 17948252
- Repeated exposure to low dose malathion increased brain AChE activity and altered antioxidant levels. PMID: 18341513
- Data show that acetylcholine esterase enzyme activity shows a significant decrease in cerebral cortex and a increase in the muscle of experimental hypoxic neonatal rats. PMID: 18406032
- Although the mechanisms of action of GAA on AChE activity and on memory are unclear, these findings suggest that the accumulation of GAA found in patients with GAMT-deficiency may be one of the mechanisms involved in neural dysfunction. PMID: 18437545
- Tanshinone modulates AChE and NOS proteins concentrations in the hippocampus of AD rats. This may have therapeutic potential in AD rats. PMID: 18980714
- Protein CutA undergoes an unusual transfer into the secretory pathway and affects the folding, oligomerization, and secretion of acetylcholinesterase. PMID: 19049969
- cell death due to paraoxon not AChE dependent PMID: 19378919
- Data show that after denervation, the expression of PRiMA, AChE(T) and G(4) AChE decreased markedly in the spinal cord, and in fast- and slow-twitch muscles. PMID: 19490106
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亚细胞定位:Cell junction, synapse. Secreted. Cell membrane; Peripheral membrane protein.; [Isoform H]: Cell membrane; Lipid-anchor, GPI-anchor; Extracellular side.
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蛋白家族:Type-B carboxylesterase/lipase family
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组织特异性:Has been found in central nervous system and muscle. Found in embryonic liver and spleen but not in adult liver.
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数据库链接:
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