Your Good Partner in Biology Research

  1. 首页
  2. 试剂盒
  3. 酶联免疫试剂盒
  4. Human Integrin αM,ITG/CD11b ELISA Kit

Human Integrin αM,ITG/CD11b ELISA Kit

  • 中文名称：
    人整合素αM型(ITG αM/CD11b)酶联免疫试剂盒
  • 货号：
    CSB-E11638h
  • 规格：
    96T/48T
  • 价格：
    ￥3200/￥2500
  • 其他：

产品详情

  • 产品描述：

    This human ITGAM ELISA kit employs the quantitative sandwich enzyme immunoassay technique to measure the levels of human ITGAM in multiple samples, including serum, plasma, or tissue homogenates. It also uses the enzyme-substrate chromogenic reaction to visualize and analyze the analyte levels through the color intensity. The intensity of the colored product is in direct proportion to the ITGAM levels in the sample and is measured at 450 nm through a microplate reader.

    ITGAM is the encoding gene for CD11b, highly expressed on the surface of innate immune cells, including macrophages and neutrophils, which together with CD18, form Mac-1 or CR3, a protein that mediates leukocyte adhesion, migration, and phagocytosis in different cells including neutrophils, monocytes, macrophages, and dendritic cells. CD11b also contributes to the phagocytosis of opsonized particles, including apoptotic cells and the immune complex. It has consistently been associated with susceptibility to systemic lupus erythematosus (SLE).

  • 别名：
    antigen CD11b (p170) ELISA Kit; Antigen CD11b p170 ELISA Kit; CD11 antigen like family member B ELISA Kit; CD11 antigen-like family member B ELISA Kit; CD11b ELISA Kit; CD11b/CD18 ELISA Kit; CD49d ELISA Kit; Cell surface glycoprotein MAC-1 subunit alpha ELISA Kit; Complement component 3 receptor 3 subunit ELISA Kit; Complement Component Receptor 3 Alpha ELISA Kit; Complement receptor type 3 ELISA Kit; Complement receptor type 3, alpha subunit ELISA Kit; CR 3 alpha chain (CR3A) ELISA Kit; CR 3 alpha chain ELISA Kit; CR-3 alpha chain ELISA Kit; CR3 ELISA Kit; CR3A ELISA Kit; F730045J24Rik ELISA Kit; Integrin Alpha M ELISA Kit; Integrin alpha M chain ELISA Kit; Integrin alpha-M ELISA Kit; Integrin beta 2 alpha subunit ELISA Kit; Integrin subunit alpha M ELISA Kit; integrin, alpha M (complement component 3 receptor 3 subunit) ELISA Kit; ITAM_HUMAN ELISA Kit; ITGAM ELISA Kit; Leukocyte adhesion receptor MO1 ELISA Kit; Ly-40 ELISA Kit; MAC 1 ELISA Kit; Mac-1a ELISA Kit; MAC1 ELISA Kit; Mac1, alpha subunit ELISA Kit; MAC1A ELISA Kit; Macrophage antigen alpha polypeptide ELISA Kit; MGC117044 ELISA Kit; Mo1 ELISA Kit; Mo1, alpha subunit ELISA Kit; MO1A ELISA Kit; Neutrophil adherence receptor alpha M subunit ELISA Kit; Neutrophil adherence receptor ELISA Kit; SLEB6 ELISA Kit
  • 缩写：
  • Uniprot No.：
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    0.78 ng/mL-50 ng/mL
  • 灵敏度：
    0.195 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Signal Transduction
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human ITG/CD11b in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %100
    Range %95-104
    1:2Average %98
    Range %94-102
    1:4Average %92
    Range %87-98
    1:8Average %89
    Range %85-96
  • 回收率：
    The recovery of human ITG/CD11b spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9289-94
    EDTA plasma (n=4)9692-102
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    502.439 2.569 2.504 2.320
    251.933 1.987 1.960 1.776
    12.51.485 1.498 1.492 1.308
    6.250.999 1.009 1.004 0.820
    3.120.575 0.597 0.586 0.402
    1.560.394 0.374 0.384 0.200
    0.780.308 0.316 0.312 0.128
    00.183 0.185 0.184 　
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Integrin ITGAM/ITGB2 is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles and pathogens. It is identical with CR-3, the receptor for the iC3b fragment of the third complement component. It probably recognizes the R-G-D peptide in C3b. Integrin ITGAM/ITGB2 is also a receptor for fibrinogen, factor X and ICAM1. It recognizes P1 and P2 peptides of fibrinogen gamma chain. Regulates neutrophil migration. In association with beta subunit ITGB2/CD18, required for CD177-PRTN3-mediated activation of TNF primed neutrophils. May regulate phagocytosis-induced apoptosis in extravasated neutrophils. May play a role in mast cell development. Required with TYROBP/DAP12 in microglia to control production of microglial superoxide ions which promote the neuronal apoptosis that occurs during brain development.
  • 基因功能参考文献：
    1. A significant reduction of CD11b expression on monocytes and granulocytes, as a sign of reduced cellular inflammation, was achieved by treatment of atrial fibrillation using pulmonary vein isolation. PMID: 28073885
    2. Expression of Mac-1 on the surface of human embryonic kidney (HEK) 293 cells induced their adhesion and migration to PTN. Accordingly, PTN promoted Mac-1-dependent cell spreading and initiated intracellular signaling manifested in phosphorylation of Erk1/2. PMID: 28939773
    3. CD11b rs1143679 polymorphism appears to be associated with risk for SLE in the Han Chinese population, and may play an important role in the development of lupus nephritis. PMID: 29207897
    4. alphaMbeta2 uses its alphaI domain to bind iC3b at the thioester domain and simultaneously interacts through a region near the alphaM beta-propeller and beta2 betaI domain with a region of the C3c moiety near the C345C domain. PMID: 28292891
    5. Up-regulation of granulocyte and monocyte CD11b during plasma separation was C5-dependent. PMID: 29575196
    6. This study shown the ITGAM expression in Microglia and Astrocytes in stroke, Alzheimer's disease, and Lewy body dementia. PMID: 28398520
    7. CD11b expression on polymorphonuclear leukocytes from patients with ankylosing spondylitis stimulated with lipopolysaccharide is not different to that of healthy controls. PMID: 24373176
    8. The use of CD33(+)CD11b(+)HLA-DR(-) cells as a predictive and prognostic biomarker. PMID: 27178742
    9. Bone marrow mesenchymal stromal cells induce the rapid differentiation of CD11b+ myeloid cells from bone marrow progenitors, and such an activity requires the expression of nitric oxide synthase-2. PMID: 28183849
    10. demonstrate for the first time a dependency of monocyte-platelet aggregates and CD11b expression on monocytes in the presence of atrial fibrillation (AF) and support the notion of a close relationship between AF, thrombogenesis and inflammation PMID: 26763077
    11. Increased CD11b+ and CD200+ monocytes in those with a strong presence of BVN indicate that BVN development may be associated with retinal injury and a VEGF-mediated process that is either reflected or propelled by systemic changes in monocytes. PMID: 29049725
    12. This study, we provide evidence supporting a crucial pathogenic role for alphaM-integrin (CD11b) in acute inflammatory demyelinating polyradiculoneuropathy mononuclear leukocyte trafficking. PMID: 27460017
    13. TLR-stimulated macrophages from CD11B SNP carriers showed increased basal expression of IFN regulatory factor 7 (IRF7) and IFN-beta, as well as increased nuclear exclusion of FOXO3, which was suppressed by LA1-dependent activation of CD11b. PMID: 28263189
    14. FRbeta as a novel CD11b/CD18 regulator for trafficking and homing of a subset of macrophages on collagen. PMID: 27534550
    15. this study shows that upregulation of CD11b after stimulation with IL-8 is more pronounced in adults than in neonates, whereas fMLP induces changes in receptor expression that are of the same magnitude in neutrophils from neonates as from adults PMID: 27606963
    16. Ingestion of amino acid supplement did not influence CD11b expression. PMID: 27384580
    17. This metaanalysis indicates that polymorphisms in the pathways of immune complex clearance, such as the FcgammaRIIIa, FcgammaRIIIb, and ITGAM genotypes, are potential susceptibility genes for neuropsychiatric systemic lupus erythematosus. PMID: 26773105
    18. Monocyte-platelet aggregates and CD11b expression on monocytes and granulocytes are increased in atrial fibrillation patients with proven thrombus formation. They seem to be appropriate biomarkers for stratification of thromboembolic risk in patients with AF. PMID: 26411420
    19. CD11b immunophenotyping identifies inflammatory profiles in the mouse and human lungs. PMID: 26422753
    20. There is direct interaction between C3(H2O) and CD11b. Contact-activated C3(H2O) is a novel ligand for CD11b/CD18 that mediates platelet-PMN complex formation and the binding of platelet-derived microparticles to PMNs. PMID: 26293614
    21. increased in neonates with abnormal neuroimaging and/or severe neonatal encephalopathy PMID: 25655529
    22. Data show that secreted aspartic protease 2 (Sap2) of Candida albicans inactivates human macrophage complement factor H (factor H), factor H-receptors CD11b/CD18 and CD11c/CD18. PMID: 26306739
    23. we confirmed the similarities between epithelial ovarian cancer and fallopian tube, normal and adenocarcinoma using FOLR1, FOLR2, CD68 and CD11b markers PMID: 25971554
    24. While total surface expression of CD11b and L-selectin on neutrophils was largely unaffected PMID: 26361072
    25. CD11b expression level might be considered a prognostic biomarker for Acute Myeloid Leukemia patients. [meta-analysis] PMID: 26309131
    26. analysis of neutrophil and monocyte expression of toll-like receptor 4, CD11b and reactive oxygen intermediates, and correlation with neuroimaging outcomes in preterm infants PMID: 25826119
    27. The rs1143679 polymorphism is associated with systemic lupus erythematosus susceptibility in different ethnic groups and with lupus nephritis in Europeans. No association was found with rheumatoid arthritis. [Meta-Analysis] PMID: 25315704
    28. the results identify dynorphins A and B as novel ligands for Mac-1. PMID: 26036990
    29. A novel expanding CD11b+ conventional dendritic cell subpopulation dominates the infiltrating renal inflammatory milieu, localizing to the glomeruli, in lupus nephritis. PMID: 26512111
    30. Modification of Levels of Adhesion Molecule Expression of Human Innate Immune Cells by Glycopolymers of Marine Bacteria PMID: 26852488
    31. study supports the crucial role of GABP in myeloid cell differentiation and identified ITGAM/CD11b as a novel GABP target gene PMID: 26170143
    32. Data indicate that Candida albicans beta-Glucan induces high IL-1 receptor antagonist (IL-1Ra) levels, independently from Dectin-1/complement receptor 3 (CR3). PMID: 25461401
    33. A novel association of common variable immunodeficiency have been found with rare variants at the FUS/ITGAM (CD11b) locus on 16p11.2. PMID: 25678086
    34. role of CD11b/CD18 in priming of human leukocytes by endotoxin glycoforms from Escherichia coli PMID: 25365491
    35. Il-6-induced recruitment of CD11b+ CD14+ HLA-DR- myeloid-derived suppressor cells is associated with progression and poor prognosis in squamous cell carcinoma of the esophagus. PMID: 25238263
    36. Results show the functional importance of integrin alpha M (ITGAM) in systemic lupus erythematosus (SLE) pathogenesis through impaired phagocytosis. PMID: 24886912
    37. Examination of CR3 ( CD11b/CD18)mutant variants and mass spectrometry analysis of the N-glycosylation pattern of CR3 revealed that N-glycans located in the C-terminal part of the CD11b subunit are involved in binding and cytotoxic activity of CyaA. PMID: 25554420
    38. newly revealed recognition specificity of alpha(M)beta2 toward unfolded protein segments and cationic proteins and peptides suggests that alpha(M)beta2 may serve as a previously proposed "alarmin" receptor with important roles in innate host defense PMID: 25613106
    39. The HNA-1b allotype influences the Fc gamma RIIIb cooperation with Fc gamma RIIa, but not with CR3. PMID: 24945596
    40. The altered expression levels of ITGAM and FCgammaRIIIA mRNA in systemic lupus erythematosus patients and their correlations with clinical data suggest that ITGAM and FCgammaRIIIA may play a role in this disease. PMID: 23708937
    41. The transcript levels of ITGAM significantly decreased for the systemic lupus erythematosus risk allele (A) relative to the non-risk allele (G), in a dose-dependent fashion. PMID: 24608226
    42. A new genome-wide significant association between ITGAM-ITGAX and IgA nephropathy. PMID: 25305756
    43. A negative correlation was found between CD11b percentage and postinfarct left ventricular ejection fraction. PMID: 25136078
    44. hnRNP K in PU.1-containing complexes recruited at the CD11b promoter: a distinct role in modulating granulocytic and monocytic differentiation of AML-derived cells. PMID: 25005557
    45. This suggests that FcgammaRIIIb signals in association with macrophage-1 Ag. PMID: 25024378
    46. Although CD11b was more frequently expressed in blast cells of patients with intermediate and unfavorable cytogenetic risk groups, this feature did not translate into different clinical outcome. PMID: 24782118
    47. circulating trisomy 12 CLL cells also have increased expression of the integrins CD11b, CD18, CD29, and ITGB7, and the adhesion molecule CD323. PMID: 24829201
    48. integrin alpha9beta1/CD11B levels in circulating neutrophils are significantly elevated in pneumonia patients PMID: 23257917
    49. genetic polymorphism is associated with systemic lupus erythematosus in Brazilian patients PMID: 24269694
    50. Complement receptor 3 (CR3) inhibition impairs phagocytosis of serum-opsonized zymosan but has little impact on phagocytosis of unopsonized zymosan in the absence or presence of lipopolysaccharide. PMID: 24726062

    显示更多

    收起更多

  • 相关疾病：
    Systemic lupus erythematosus 6 (SLEB6)
  • 亚细胞定位：
    Cell membrane; Single-pass type I membrane protein. Membrane raft; Single-pass type I membrane protein.
  • 蛋白家族：
    Integrin alpha chain family
  • 组织特异性：
    Predominantly expressed in monocytes and granulocytes. Expressed in neutrophils (at protein level).
  • 数据库链接：

    HGNC: 6149

    OMIM: 120980

    KEGG: hsa:3684

    STRING: 9606.ENSP00000441691

    UniGene: Hs.172631