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Bovine Parathyroid hormone(PTH) ELISA kit

  • 中文名称：
    牛甲状旁腺素(PTH)酶联免疫试剂盒
  • 货号：
    CSB-EL018987BO
  • 规格：
    96T/48T
  • 价格：
    ￥4200/￥3000
  • 其他：

产品详情

  • 产品描述：

    This Bovine PTH ELISA Kit was designed for the quantitative measurement of Bovine PTH protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 31.25 pg/mL-2000 pg/mL and the sensitivity is 7.8 pg/mL.

  • 别名：
    PTH ELISA Kit; Parathyroid hormone ELISA Kit; PTH ELISA Kit; Parathyrin ELISA Kit
  • 缩写：
  • Uniprot No.：
  • 种属：
    Bos taurus (Bovine)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    31.25 pg/mL-2000 pg/mL
  • 灵敏度：
    7.8 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Signal Transduction
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of bovine PTH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)
    1:1 Average % 91
    Range % 86-99
    1:2 Average % 96
    Range % 90-110
    1:4 Average % 90
    Range % 83-99
    1:8 Average % 95
    Range % 85-105
  • 回收率：
    The recovery of bovine PTH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 92 88-98
    EDTA plasma (n=4) 96 90-110
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected
    2000 2.626 2.603 2.615 2.498
    1000 2.365 2.325 2.345 2.228
    500 1.986 1.992 1.989 1.872
    250 1.502 1.486 1.494 1.377
    125 0.841 0.835 0.838 0.721
    62.5 0.486 0.474 0.480 0.363
    31.25 0.371 0.375 0.373 0.256
    0 0.115 0.119 0.117 　
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    PTH elevates calcium level by dissolving the salts in bone and preventing their renal excretion. Stimulates [1-14C]-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblastic cells.
  • 基因功能参考文献：
    1. These data highlight the ability of PTH to phosphorylate beta-catenin directly via PKA. PMID: 26851123
    2. These data suggest that prostaglandin E2 acting via EP4R on bone marrow macrophages committed to the osteoblast cell lineage, stimulated secretion of a factor or factors that acted to suppress PTH-stimulated osteoblast differentiation. PMID: 23639875
    3. These results suggest that in vivo PTH treatment increased in vitro osteoclastogenesis and resorption without altering the number of osteoclast precursors. PMID: 21611963
    4. Osteoblast 2-deoxyglucose uptake and glycogen synthesis were increased after exposure to low concentrations (0.1 nmol/l and above) of PTH. PMID: 21076856
    5. Parathyroid hormone stimulated growth and decreased Col-X deposition via phosphotidylinositol-3,4,5 triphosphate kinase and mitogen activating protein kinase pathways in avian sterna. PMID: 19957341
    6. likely involvement of the Sp family in regulating PTH gene expression through interactions with an Sp1 DNA element in the hormone's promoter. PMID: 12810570
    7. alternative cis-acting protein-binding elements may determine the regulation of PTH mRNA stability in response to changes in serum calcium and phosphate PMID: 15824859
    8. a possible role for the Wnt signaling pathway in PTH actions in bone PMID: 15962290
    9. Data suggest that calcium-mediated destabilization of parathyroid hormone mRNA requires gene transcription and involves increases in cytosolic Ca. PMID: 18096993

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  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    Parathyroid hormone family
  • 数据库链接：

    KEGG: bta:280903

    STRING: 9913.ENSBTAP00000025399

    UniGene: Bt.95