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Canine Retinol binding protein 4(RBP-4) ELISA Kit

  • 中文名称:
    犬视黄醇结合蛋白4(RBP-4)酶联免疫试剂盒
  • 货号:
    CSB-E16500c
  • 规格:
    96T/48T
  • 价格:
    ¥3600/¥2500
  • 其他:

产品详情

  • 缩写:
    RBP-4
  • Uniprot No.:
  • 种属:
    Canis lupus familiaris (Dog) (Canis familiaris)
  • 检测范围:
    Request Information
  • 灵敏度:
    Request Information
  • 反应时间:
    1-5h
  • 样本体积:
    50-100ul
  • 检测波长:
    450 nm
  • 研究领域:
    Signal Transduction
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of canine RBP-4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:1 Average % 91  
    Range % 86-96  
    1:2 Average % 97  
    Range % 92-103  
    1:4 Average % 92  
    Range % 87-99  
    1:8 Average % 92  
    Range % 85-97  
  • 回收率:
    The recovery of canine RBP-4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 93 89-96  
    EDTA plasma (n=4) 97 95-100  
                 
                 
  • 标准曲线:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    μg/ml OD1 OD2 Average Corrected  
    80 1.944 2.111 2.028 1.867  
    40 1.326 1.240 1.283 1.122  
    20 0.777 0.787 0.782 0.621  
    10 0.445 0.430 0.438 0.277  
    5 0.299 0.286 0.293 0.132  
    2.5 0.231 0.242 0.237 0.076  
    1.25 0.201 0.206 0.204 0.043  
    0 0.158 0.164 0.161    
  • 货期:
    3-5 working days

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