Your Good Partner in Biology Research

  1. 首页
  2. 试剂盒
  3. 酶联免疫试剂盒
  4. Chicken Interleukin 10,IL-10 ELISA Kit

Chicken Interleukin 10,IL-10 ELISA Kit

  • 中文名称：
    鸡白介素10(IL-10)酶联免疫试剂盒
  • 货号：
    CSB-E12835C
  • 规格：
    96T/48T
  • 价格：
    ￥3800/￥2500
  • 其他：

产品详情

  • 产品描述：
        IL10（Interleukin-10，白细胞介素10）是一种由多种免疫细胞产生的细胞因子，具有抗炎和免疫调节的作用。IL-10主要由T细胞、B细胞、单核细胞和巨噬细胞等细胞合成，并对免疫细胞和炎症反应起调节作用。IL-10能够抑制炎症细胞产生炎症介质，减轻炎症反应，对免疫系统的平衡和调节具有重要作用。IL-10还参与了一系列免疫相关疾病的发生和发展，如自身免疫病、炎症性肠病和过敏性疾病等。
        j9九游会登录入口首页生物所提供的Chicken Interleukin 10,IL-10 ELISA Kit属于ELISA检测试剂盒，采用竞争法定量检测鸡血清、血浆、组织匀浆样本中的IL10，其灵敏度为0.5 pg/mL，检测范围为1 pg/mL-200 pg/mL。
     
  • 别名：
    IL10Interleukin-10 ELISA Kit; IL-10 ELISA Kit
  • 缩写：
  • Uniprot No.：
  • 种属：
    Gallus gallus (Chicken)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    1 pg/mL-200 pg/mL
  • 灵敏度：
    0.5 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Immunology
  • 测定原理：
    quantitative
  • 测定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<15%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<15%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of chicken IL-10 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:1 Average % 96  
    Range % 90-101  
    1:2 Average % 97  
    Range % 94-105  
    1:4 Average % 95  
    Range % 89-100  
    1:8 Average % 92  
    Range % 87-96  
  • 回收率：
    The recovery of chicken IL-10 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 94 90-98  
    EDTA plasma (n=4) 97 93-101  
                 
                 
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average 　  
    200 0.155 0.152 0.154 　  
    50 0.334 0.348 0.341 　  
    16 0.515 0.502 0.509 　  
    4 0.969 0.975 0.972 　  
    1 1.436 1.463 1.450 　  
    0 2.658 2.667 2.663 　  
  • 数据处理：
  • 货期：
    3-5 working days

引用文献

产品评价

靶点详情

  • 最新研究进展：
    IL-10是一种抗炎因子，对调节炎症反应和维持免疫平衡具有重要作用。最新研究发现，IL-10参与许多自身免疫性疾病的发病机制，并且IL-10的水平可能作为这些疾病的生物标记物。此外，IL-10在肿瘤微环境中也发挥作用，可以通过抑制炎症反应和免疫应答来促进肿瘤生长。
  • 功能：
    Major immune regulatory cytokine that acts on many cells of the immune system where it has profound anti-inflammatory functions, limiting excessive tissue disruption caused by inflammation. Mechanistically, IL10 binds to its heterotetrameric receptor comprising IL10RA and IL10RB leading to JAK1 and STAT2-mediated phosphorylation of STAT3. In turn, STAT3 translocates to the nucleus where it drives expression of anti-inflammatory mediators. Targets antigen-presenting cells (APCs) such as macrophages and monocytes and inhibits their release of pro-inflammatory cytokines including granulocyte-macrophage colony-stimulating factor /GM-CSF, granulocyte colony-stimulating factor/G-CSF, IL-1 alpha, IL-1 beta, IL-6, IL-8 and TNF-alpha. Interferes also with antigen presentation by reducing the expression of MHC-class II and co-stimulatory molecules, thereby inhibiting their ability to induce T cell activation. In addition, controls the inflammatory response of macrophages by reprogramming essential metabolic pathways including mTOR signaling.
  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    IL-10 family
  • 组织特异性：
    Expressed predominantly in bursa of Fabricius and cecal tonsils with low levels in thymus, liver and lung.
  • 数据库链接：