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Chicken Interleukin 17,IL-17 ELISA Kit

  • 中文名称:
    鸡白介素17(IL-17)酶联免疫试剂盒
  • 货号:
    CSB-E04607Ch
  • 规格:
    96T/48T
  • 价格:
    ¥3200/¥2500
  • 其他:

产品详情

  • 产品描述:

    This Chicken IL17 ELISA Kit was designed for the quantitative measurement of Chicken IL17 protein in serum, plasma, tissue homogenates. It is a Competitive ELISA kit, its detection range is 150 pg/mL-6000 pg/mL and the sensitivity is 150 pg/mL.

  • 缩写:
    IL-17
  • Uniprot No.:
  • 种属:
    Gallus gallus (Chicken)
  • 样本类型:
    serum, plasma, tissue homogenates
  • 检测范围:
    150 pg/mL-6000 pg/mL
  • 灵敏度:
    150 pg/mL
  • 反应时间:
    1-5h
  • 样本体积:
    50-100ul
  • 检测波长:
    450 nm
  • 研究领域:
    Immunology
  • 测定原理:
    quantitative
  • 测定方法:
    Competitive
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<15%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<15%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of chicken IL-17 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:1 Average % 90  
    Range % 82-100  
    1:2 Average % 95  
    Range % 90-100  
    1:4 Average % 96  
    Range % 90-101  
    1:8 Average % 91  
    Range % 85-98  
  • 回收率:
    The recovery of chicken IL-17 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 91 85-96  
    EDTA plasma (n=4) 92 86-98  
                 
                 
  • 标准曲线:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average    
    0 1.914 2.027 1.971    
    150 1.306 1.329 1.318    
    450 1.039 1.102 1.071    
    1380 0.755 0.702 0.729    
    3000 0.484 0.462 0.473    
    6000 0.351 0.348 0.350    
  • 数据处理:
  • 货期:
    3-5 working days

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