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  4. Chicken Interleukin 4,IL-4 ELISA Kit

Chicken Interleukin 4,IL-4 ELISA Kit

  • 中文名称：
    鸡白介素4(IL-4)酶联免疫试剂盒
  • 货号：
    CSB-E06756Ch
  • 规格：
    96T/48T
  • 价格：
    ￥3800/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Chicken IL-4 ELISA Kit was designed for the quantitative measurement of Chicken IL-4 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 12.5 pg/mL-800 pg/mL and the sensitivity is 3.12 pg/mL.

  • 缩写：
    IL-4
  • Uniprot No.：
  • 种属：
    Gallus gallus (Chicken)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    12.5 pg/mL-800 pg/mL
  • 灵敏度：
    3.12 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Immunology
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of chicken IL-4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %89
    Range %80-95
    1:2Average %94
    Range %88-101
    1:4Average %93
    Range %86-106
    1:8Average %95
    Range %82-100
  • 回收率：
    The recovery of chicken IL-4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9590-97
    EDTA plasma (n=4)9285-96
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    8001.726 1.825 1.776 1.665
    4001.336 1.383 1.360 1.249
    2001.060 0.981 1.021 0.910
    1000.714 0.706 0.710 0.599
    500.481 0.475 0.478 0.367
    250.307 0.292 0.300 0.189
    12.50.227 0.214 0.221 0.110
    00.108 0.113 0.111 　
  • 数据处理：
  • 货期：
    3-5 working days

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