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  4. Dog Osteocalcin/bone gla protein,OT/BGP ELISA Kit

Dog Osteocalcin/bone gla protein,OT/BGP ELISA Kit

  • 中文名称：
    犬骨钙素/骨谷氨酸蛋白(OT/BGP)酶联免疫试剂盒
  • 货号：
    CSB-E13345c
  • 规格：
    96T/48T
  • 价格：
    ￥4200/￥3000
  • 其他：

产品详情

  • 产品描述：

    This Dog BGLAP ELISA Kit was designed for the quantitative measurement of Dog BGLAP protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.312 ng/mL-20 ng/mL and the sensitivity is 0.078 ng/mL .

  • 别名：
    BGLAP ELISA Kit; Osteocalcin ELISA Kit; Bone Gla protein ELISA Kit; BGP ELISA Kit; Gamma-carboxyglutamic acid-containing protein ELISA Kit
  • 缩写：
    BGLAP
  • Uniprot No.：
  • 种属：
    Canis lupus familiaris (Dog) (Canis familiaris)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    0.312 ng/mL-20 ng/mL
  • 灵敏度：
    0.078 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Metabolism
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of dog OT/BGP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %96
    Range %87-102
    1:2Average %92
    Range %89-98
    1:4Average %89
    Range %84-96
    1:8Average %90
    Range %86-99
  • 回收率：
    The recovery of dog OT/BGP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 8984-96
    EDTA plasma (n=4)10295-109
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    203.105 3.004 3.055 2.953
    102.654 2.674 2.664 2.562
    51.878 1.889 1.884 1.782
    2.51.036 1.105 1.071 0.969
    1.250.468 0.500 0.484 0.382
    0.6250.261 0.273 0.267 0.165
    0.3120.158 0.164 0.161 0.059
    00.103 0.100 0.102 　
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Constitutes 1-2% of the total bone protein. It binds strongly to apatite and calcium.
  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    Osteocalcin/matrix Gla protein family
  • 数据库链接：