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  4. Human Kidney injury molecule 1,Kim-1 ELISA Kit

Human Kidney injury molecule 1,Kim-1 ELISA Kit

  • 中文名称：
    人肾损伤分子1(Kim-1)酶联免疫试剂盒
  • 货号：
    CSB-E08807h
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：
        Kim-1，肾上皮细胞跨膜蛋白1（Kidney Injury Molecule-1），属于肾小管上皮细胞表面分子，是急性肾损伤的重要标志物。Kim-1在正常情况下表达较低，但在肾小管上皮细胞受到损伤后，其表达会显著上调。因此，Kim-1可作为早期诊断急性肾损伤的敏感指标，对于肾脏疾病的诊断和监测具有重要意义。
        j9九游会登录入口首页生物所提供的Human Kidney injury molecule 1,Kim-1 ELISA Kit属于ELISA检测试剂盒，采用双抗夹心法定量检测人血清、尿液、组织匀浆样本中的Kim-1，其灵敏度为0.043 ng/mL，检测范围为0.312 ng/mL-20 ng/mL。
     
  • 别名：
    CD365 ELISA Kit; HAVCR 1 ELISA Kit; HAVCR ELISA Kit; HAVcr-1 ELISA Kit; Havcr1 ELISA Kit; Hepatitis A virus cellular receptor 1 ELISA Kit; Kidney injury molecule 1 ELISA Kit; KIM 1 ELISA Kit; KIM-1 ELISA Kit; T cell immunoglobin domain and mucin domain protein 1 ELISA Kit; T cell immunoglobulin mucin family member 1 ELISA Kit; T cell immunoglobulin mucin receptor 1 ELISA Kit; T-cell immunoglobulin and mucin domain-containing protein 1 ELISA Kit; T-cell membrane protein 1 ELISA Kit; TIM ELISA Kit; TIM-1 ELISA Kit; TIM1 ELISA Kit; TIMD 1 ELISA Kit; TIMD-1 ELISA Kit; TIMD1 ELISA Kit; TIMD1_HUMAN ELISA Kit
  • 缩写：
    Kim-1
  • Uniprot No.：
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, urine, tissue homogenates
  • 检测范围：
    0.312 ng/mL-20 ng/mL
  • 灵敏度：
    0.043 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Immunology
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human Kim-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:1 Average % 89  
    Range % 82-96  
    1:2 Average % 104  
    Range % 99-109  
    1:4 Average % 100  
    Range % 94-106  
    1:8 Average % 92  
    Range % 85-98  
  • 回收率：
    The recovery of human Kim-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 102 96-108  
    EDTA plasma (n=4) 90 84-96  
                 
                 
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average Corrected  
    20 2.409 2.565 2.487 2.317  
    10 1.599 1.518 1.559 1.389  
    5 1.012 1.003 1.008 0.838  
    2.5 0.587 0.578 0.583 0.413  
    1.25 0.433 0.463 0.448 0.278  
    0.625 0.302 0.304 0.303 0.133  
    0.312 0.231 0.235 0.233 0.063  
    0 0.174 0.165 0.170 　  
  • 本试剂盒所含材料：
      • A micro ELISA plate ---The 96-well plate has been pre-coated with an anti-human Kim-1 antibody.
      • Six vials standard (5 ml/bottle) ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
      • One vial HRP-conjugated Kim-1 antibody (6 ml/bottle) ---Bind to the MSTN in the samples or standards and react with the substrate to make the solution chromogenic.
      • Substrate A (1 x 7 ml)
      • Substrate B (1 x 7 ml)
      • One vial Stop Solution (7ml/bottle) ---Stop the color reaction. The solution color immediately turns from blue to yellow.
      • Four Adhesive Strips (For 96 wells) ---Cover the microplate when incubation.
      • An instruction manual

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  • 本试剂盒不含材料：
      • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 600 nm or 630 nm.
      • An incubator can provide stable incubation conditions up to 37°C±5°C.
      • Centrifuge
      • Vortex
      • Squirt bottle, manifold dispenser, or automated microplate washer
      • Absorbent paper for blotting the microtiter plate
      • 50-300ul multi-channel micropipette
      • Pipette tips
      • Single-channel micropipette with different ranges
      • 100ml and 500ml graduated cylinders
      • Deionized or distilled water
      • Timer
      • Test tubes for dilution

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  • 数据处理：
  • 货期：
    3-5 working days

引用文献

产品评价

靶点详情

  • 最新研究进展：
    Kim-1是一种跨膜蛋白，也称为T-cell immunoglobulin mucin-1（TIM-1），其在人体中广泛分布于不同的组织和细胞类型中。最近的研究表明，Kim-1在肾脏疾病的发展和进展中起着重要作用，可以作为一种生物标志物用于肾脏疾病的早期诊断和监测。此外，Kim-1在其他一些疾病中也发挥作用，例如肝病、肺部疾病等。
  • 功能：
    May play a role in T-helper cell development and the regulation of asthma and allergic diseases. Receptor for TIMD4. May play a role in kidney injury and repair.; (Microbial infection) Acts as a receptor for Hepatitis A virus.; (Microbial infection) Acts as a receptor for Ebolavirus and Marburg virus by binding exposed phosphatidyl-serine at the surface of virion membrane. Serves as a dual receptor for Ebolavirus by also interacting with envelope glycoprotein GP.; (Microbial infection) Acts as a receptor for Dengue virus by binding exposed phosphatidyl-serine at the surface of virion membrane.; (Microbial infection) Acts as a receptor for Zika virus by binding to envelope protein E.
  • 基因功能参考文献：
    1. neither the inflammatory biomarker calprotectin, nor the tubular biomarkers NGAL and KIM-1, provide robust prognostic information on the loss of renal function in a heterogeneous CKD population. All of them, however, are candidate prognostic biomarkers in primarily inflammatory renal diseases. PMID: 30078006
    2. High KIM1 expression is associated with Clear Cell Renal Cell Carcinoma. PMID: 29295730
    3. Human oligodendrocytes undergo apoptosis when exposed to Sema4A and take up H-ferritin for meeting iron requirements and that these functions are mediated via the Tim-1 receptor. H-ferritin can block Sema4A-mediated cytotoxicity. Sema4A is detectable in the CSF of multiple sclerosis patients and HIV-seropositive persons and can induce oligodendrocyte cell death. PMID: 29457657
    4. Elevated plasma NT-proBNP and KIM-1 in patients with stage 5 chronic kidney disease (CKD5) undergoing maintenance hemodialysis (HD) and HF (+) (heart failure)CKD5-HD patients suggest that natriuretic peptides and KIM-1 may contribute to the pathogenesis of HF in CKD5-HD patients. PMID: 28990414
    5. These findings suggest that the TIMD4-HAVCR1 variants may be the genetic risk factors for coronary heart disease and ischemic strok PMID: 29208769
    6. These studies provide evidence that, in the absence of a functional alpha-dystroglycan, TIM-1 mediates the entry of Lassa virus pseudoviral particles through interactions of virions with the IgV PtdSer-binding pocket of TIM-1. PMID: 29875238
    7. total flavone of Desmodium styracifolium inhibited HK-2 cell apoptosis and autophagy by regulating KIM-1 via p38/MAPK pathway. PMID: 29071538
    8. Polymorphism +4259A>C in exon 3 of the TIM-3 gene is associated with susceptibility to multiple sclerosis but polymorphism -1637C>T in the promoter region of TIM-1 is not. PMID: 29141799
    9. High KIM1 expression is associated with decreased proximal tubular epithelial cell function. PMID: 29045706
    10. findings show that TIM1 is not an essential cellular receptor for hepatitis A virus entry into cultured cells PMID: 28874468
    11. Urinary excretion of KIM-1 correlates with blood lead levels better than other traditional renal injury biomarkers, including N-acetyl-beta-(D)-glucosaminidase, alpha1-microglobulin, and beta2-microglobulin. PMID: 27966578
    12. promising results were obtained for circulating KIM-1 prediction of renal outcomes in type 1 diabetes--{REVIEW} PMID: 27771693
    13. data show that in addition to the phospholipid-binding function of HAVCR1, the enhancement of HCV infection involves other determinants in the IgV domain of HAVCR1. These findings expand the repertoire of molecules that HCV uses for cell entry, adding to the already complex mechanism of HCV infection and pathogenesis. PMID: 29321304
    14. Review/Meta-analysis: suggest that TIM-1-416G[C single nucleotide polymorphism is associated with the risk of asthma for the Asian ethnicity in the codominant (G/G vs. G/C) and dominant (G/G vs. G/C + C/C) genetic models. PMID: 28434117
    15. Urinary NGAL is a good predictor of mortality in established pediatric acute kidney injury (AKI) of heterogeneous etiology and outperforms calprotectin and KIM-1. Urinary calprotectin and KIM-1 predict renal replacement therapy requirement in pediatric AKI. PMID: 28409285
    16. A panel consisting of IGFBP1, KIM1, GCLC and GSTM1 genes could be used in combination for early screening of CKDu, whereas these genes in addition with FN1, IGFBP3 and KLK1 could be used to monitor progression of CKDu. The regulation of these genes has to be studied on larger populations to validate their efficiency for further clinical use. PMID: 28103909
    17. Quantification of TIM-3 and KIM-1 mRNA expressions, along with KIM-1 protein measurements in urine and blood could be employed as promising tools for noninvasive diagnosis of allograft dysfunction. PMID: 28757398
    18. Report detection of drug-induced acute kidney injury in humans by combining the sensitivity of urinary KIM-1 along with urinary miR-21, -200c, and -423. PMID: 27122240
    19. measurement of urinary and renal KIM-1 level may be helpful to evaluate severity of renal pathological damage and prognosis in adult Henoch-Schonlein purpura patients with nephritis PMID: 29122357
    20. Urine KIM-1 level in acute kidney injury patients was significantly higher than that in the healthy controls. PMID: 28212617
    21. PCNSL is characterized by frequent Tim-1 expression, and its soluble form in CSF may become a useful biomarker for PCNSL. PMID: 27709813
    22. TIM-1 is a unique marker for the identification of a human IL-10(+) Breg subpopulation which is partially superimposed with transitional B cells PMID: 28103916
    23. in the current meta-analysis, based on ten prospective studies involving 29366 participants, we evaluated the role of urinary tubular injury markers (NGAL, KIM-1 and NAG) in predicting clinical outcomes including CKD stage 3, end stage renal disease and mortality. PMID: 27907168
    24. Urinary KIM-1 levels in the acute kidney injury (AKI) preterm infant group were not significantly higher than in no-AKI group on day of life 1, 3 and 7. PMID: 27020372
    25. We conclude that KIM-1 might serve as a sensitive biomarker to screen children for kidney damage induced by environmental toxic agents, such as chromium and arsenic PMID: 27431456
    26. These results suggest that a 6-amino acid deletion polymorphism in the mucin domain of TIM-1 protects from HIV-1 infection with a recessive effect. PMID: 27652980
    27. The present study shows that serum and urine levels of NGAL and KIM-1 are higher in patients with acute kidney injury than in those without acute kidney injury, and that serum NGAL and the presence of chronic kidney disease are significant predictors of acute kidney injury in scrub typhus. PMID: 28419138
    28. KIM-1 increases albumin endocytosis in renal tubule epithelial cells. PMID: 26332568
    29. preliminary data indicates that KIM-1 expression may be associated with stage in Wilms Tumor PMID: 27562389
    30. Urinary KIM-1 and NAG levels were positively correlated. PMID: 27272162
    31. TLR2, Tim1, Tim3, TNF-alpha, IgE and EOS play a role in Mycoplasma pneumoniae Pneumonia-related wheezing in children. PMID: 27185658
    32. The results of our study showed that there is no significant association between TIM-1 5383-5397ins/del and Multiple sclerosis (MS) (P = 0.38); however, the frequency of CT genotype of TIM-3 -1541C>T in patient group was significantly higher than the control group, and there was a significant association between CT genotype and MS PMID: 27091308
    33. Study emphasizes a strong association of the tubular biomarker KIM-1 with chronic kidney disease progression in chronic heart failure and suggests usefulness as cardiorenal marker. PMID: 26422793
    34. KIM-1 expression also enhances ERK MAPK activation, and the modulatory effect of KIM-1 on cellular repair process is likely mediated via ERK MAPK signaling pathway. PMID: 27084535
    35. KIM-1 may mediate high glucose-induced autophagy and apoptosis in renal tubular epithelial cells. PMID: 27310004
    36. Study suggests that TIM1 -416G>C and -1454G>A single nucleotide polymorphisms are associated with dilated cardiomyopathy susceptibility and prognosis in this Han Chinese population. PMID: 27818477
    37. elevated baseline plasma KIM-1 strongly associated with risk of early progressive renal decline in type 1 diabetes PMID: 26509588
    38. Urinary KIM-1 levels increased significantly in patients with contrast-induced nephropathy. PMID: 26474236
    39. Data show that kidney injury molecule (KIM)-1 and neutrophil gelatinase-associated lipocalin (NGAL), were significantly elevated in welding workers post-exposure. PMID: 26673824
    40. High NGAL and KIM-1 levels may indicate early diabetic kidney injury; however, we did not observe any relationship between these markers and diabetic indices PMID: 26393328
    41. Kidney injury molecule-1 and neutrophil gelatinase-associated lipocalin excretion rates correlated with excretion rates of other tubular damage markers and predicted outcome in patients with idiopathic membranous nephropathy PMID: 25762211
    42. The aim of this review article is to summarize the literature data concerning KIM-1 as a potential novel marker in the early diagnosis and prediction of clinical outcome of certain cardiovascular diseases. PMID: 26697493
    43. Activation of TIM1 by exposing the cells to TIM4 significantly increased the frequency of apoptotic colon cancer cells. The expression of FasL was increased in the cancer cells after treating by TIM4. PMID: 26921445
    44. Urine KIM1 level was higher in kidney stone patients before lithotripsy than in healthy controls. PMID: 26407192
    45. Urinary levels of NGAL are more sensitive than uKIM-1 and uL-FABP levels in predicting renal scarring in vesicoureteral reflux. PMID: 26324091
    46. TIM-1 ligands increase the amount of cell surface protein, preventing its traffic to the immune synapse PMID: 26332704
    47. Urinary KIM-1 measured 24 h after the start of drug infusion has the potential to detect early AKI in pediatric patients treated with MTX or platinum-class drugs PMID: 26248472
    48. Urinary KIM-1 is a significant predictor of death in extremely low-birth-weight neonates. PMID: 26107391
    49. Urinary KIM1 is significantly higher in patients with clear cell renal carcinoma than in controls. Urinary KIM1 decreases after nephrectomy in clear cell renal carcinoma patients. PMID: 27295912
    50. decreased serum TIM-1 and TIM-3 levels and association of TIM-3 with nephritis suggest their possible role in the development and pathogenesis of systemic lupus erythematosus. PMID: 27049082

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  • 亚细胞定位：
    Membrane; Single-pass type I membrane protein.
  • 蛋白家族：
    Immunoglobulin superfamily, TIM family
  • 组织特异性：
    Widely expressed, with highest levels in kidney and testis. Expressed by activated CD4+ T-cells during the development of helper T-cells responses.
  • 数据库链接：

    HGNC: 17866

    OMIM: 606518

    KEGG: hsa:26762

    STRING: 9606.ENSP00000344844

    UniGene: Hs.129711