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  4. Human indoleamine 2,3-dioxygenase,IDO ELISA Kit

Human indoleamine 2,3-dioxygenase,IDO ELISA Kit

  • 中文名称：
    人吲哚胺2,3-双加氧酶(IDO)酶联免疫试剂盒
  • 货号：
    CSB-E09966h
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This human IDO1 ELISA Kit is suitable for qualitatively determining human concentrations in multiple biological fluids, including human serum, plasma, and tissue homogenates in vitro. IDO1 a cytosolic enzyme with a heme (Fe2+) prosthetic group that catalyzes catalyze the oxidative catabolism of the least-abundant essential amino acid, L-Trp (L-tryptophan), along the kynurenine pathway. IDO1 converts the essential amino acid Trp to kynurenine (Kyn) by cleaving the 2,3-double bond of the indole ring while molecular oxygen merges into the unsealed molecule. It has been identified as a prominent immune regulatory enzyme that can regulate immune cell activation status and phenotype through enzyme-dependent deprivation of L-Trp and its conversion into the aryl hydrocarbon receptor ligand kynurenine or IDO1-mediated signaling. IDO1 also has pro-cancer effects. Overexpression of IDO1 has been detected in more than half of tumors using IDO1-related immunosuppressive mechanisms to promote their metastasis and survival.

    This kit uses the quantitative sandwich-based enzyme immunoassay technique to measure the amount of human IDO1 in the sample. Standards and samples are respectively added to the microplate wells pre-coated with an anti-human IDO1 antibody. Biotin-labeled IDO1 antibody, HRP-avidin, and TMB substrate are pipped into the microplate in turn. The capture antibody pre-coated on the plate captures the IDO1 in the human samples. IDO1 binds to the biotinylated anti-IDO1 human monoclonal antibody. And the biotin on the biotinylated anti-IDO1 human monoclonal antibody binds to the avidin on the enzyme label, forming immune complexes. The color renders blue after the addition of the TMB substrate. The addition of the stop solution into the wells immediately turns the blue into yellow. The concentration of IDO1 in the samples is directly proportional to OD (450nm). Each manufactured lot of this ELISA kit was quality tested for criteria such as sensitivity, specificity, precision, linearity, and lot-to-lot consistency.

  • 别名：
    3-dioxygenase ELISA Kit; I23O1_HUMAN ELISA Kit; IDO 1 ELISA Kit; IDO ELISA Kit; IDO-1 ELISA Kit; IDO1 ELISA Kit; INDO ELISA Kit; indolamine 2,3 dioxygenase ELISA Kit; Indole 2 3 dioxygenase ELISA Kit; indoleamine 2 3 dioxygenase 1 ELISA Kit; indoleamine 2 3 dioxygenase ELISA Kit; Indoleamine 2,3-dioxygenase 1 ELISA Kit; Indoleamine pyrrole 2 3 dioxygenase ELISA Kit; Indoleamine-pyrrole 2 ELISA Kit
  • 缩写：
  • Uniprot No.：
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, tissue homogenates, cell lysates
  • 检测范围：
    0.78 ng/mL-50 ng/mL
  • 灵敏度：
    0.195 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Metabolism
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human IDO in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %90
    Range %85-99
    1:2Average %95
    Range %91-102
    1:4Average %89
    Range %84-94
    1:8Average %93
    Range %89-98
  • 回收率：
    The recovery of human IDO spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9791-104
    EDTA plasma (n=4)8680-90
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    502.574 2.552 2.563 2.461
    252.078 1.949 2.014 1.912
    12.51.228 1.278 1.253 1.151
    6.250.537 0.560 0.549 0.447
    3.120.326 0.340 0.333 0.231
    1.560.204 0.197 0.201 0.099
    0.780.159 0.152 0.156 0.054
    00.103 0.100 0.102 　
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Catalyzes the first and rate limiting step of the catabolism of the essential amino acid tryptophan along the kynurenine pathway. Involved in the peripheral immune tolerance, contributing to maintain homeostasis by preventing autoimmunity or immunopathology that would result from uncontrolled and overreacting immune responses. Tryptophan shortage inhibits T lymphocytes division and accumulation of tryptophan catabolites induces T-cell apoptosis and differentiation of regulatory T-cells. Acts as a suppressor of anti-tumor immunity. Limits the growth of intracellular pathogens by depriving tryptophan. Protects the fetus from maternal immune rejection.
  • 基因功能参考文献：
    1. Interference SNHG1 could inhibit the differentiation of Treg cells by promoting miR-448 expression and reducing IDO level, thereby impeding the immune escape of breast cancer . PMID: 29886172
    2. We discuss how small-molecule inhibitors of the tryptophan (Trp) catabolic enzyme indoleamine 2,3-dioxygenase (IDO) represent a vanguard of new immunometabolic adjuvants to safely enhance the efficacy of cancer immunotherapy, radiotherapy, or 'immunogenic' chemotherapy by leveraging responses to tumor neoantigens PMID: 29413421
    3. The role of IDO1-IDO2-AHR pathway in the TLR4-induced tolerogenic phenotype in human dendritic cells has been reported. PMID: 28256612
    4. High coexpression of cytoplasmic IDO1/COX2 was found to be an independent predictor of poor outcome in colorectal cancer patients. PMID: 29853736
    5. Report the crystal structures of IDO1 in complex with its substrate, Trp, an inhibitor, epacadostat, and/or an effector, indole ethanol (IDE). The data reveal structural features of the active site (Sa) critical for substrate activation; in addition, they disclose a new inhibitor-binding mode and a distinct small molecule binding site (Si). PMID: 29167421
    6. IDO decreased glycolysis and glutaminolysis by activating GCN2K, resulting in activation of AMPactivated protein kinase. PMID: 29693118
    7. this review highlights the role of indoleamine-2,3-dioxygenase in normal and pathological pregnancies PMID: 29154462
    8. Data suggest that IDO1 is constitutively expressed in insulin-secreting cells from donors without diabetes; IDO1 appears to be down-regulated in insulin-containing beta-cells from double autoantibody-positive donors and donors with recent-onset type 1 diabetes; this study was conducted on donor tissues obtained from cadavers. PMID: 29945890
    9. Twenty-nine percent (n = 2/7) of the PD-L1 positive poorly differentiated thyroid carcinomas also co-expressed IDO1 PMID: 29372535
    10. In non-ST segment elevation myocardial infarction, the tolerogenic mechanism of the immune response related to IDO production by activated monocytes derived dendritic cells is altered, supporting their role in T-cell dysregulation. PMID: 29278387
    11. These data suggest that the expression of immunosuppressive molecules, including PD-1 ligands and IDO1, by macrophage/microglia may be involved in immune evasion of lymphoma cells. PMID: 29998979
    12. Our data demonstrate that high intratumoral IDO1 mRNA levels correlate with a poor glioblastoma (GBM) patient prognosis. It also confirms the positive correlation between increased GBM IDO1 levels and human-infiltrating T cells PMID: 28751450
    13. Among the 89 patients, CD274, LAG3, and IDO1 expressions in TIICs were observed in 68.6% (61 cases), 13.5% (12), and 28.1% (25) of patients, respectively. CD274, CTLA4, and IDO1 were expressed in tumor cells of 24.7% (22 cases), 4.5% (4), and 72.0% (64) of patients, respectively. PMID: 29520442
    14. Multivariate analysis indicated indoleamine 2,3-dioxygenase (IDO) expression as independent prognostic factors in overall survival (OS). PMID: 29848687
    15. these findings suggest that IDO1 promoter methylation regulates anti-immune responses in breast cancer subtypes and could be used as a predictive biomarker for IDO1 inhibitor-based immunotherapy. PMID: 28264810
    16. main biological and actionable difference between OSCC from NSND and SD lies in the immune microenvironment, suggesting a higher clinical benefit of PD-L1 and IDO1 inhibition in OSCC from NSND PMID: 28460011
    17. An IDO1 inhibitor, epacadostat also demonstrated promising activity in combination with the PD-1 checkpoint inhibitors in other solid tumors, including melanoma, urothelial carcinoma, renal cell carcinoma, and non-small cell lung cancer PMID: 28760910
    18. This first-in-human phase I study investigated the maximum tolerated dose, safety, pharmacokinetics, pharmacodynamics, and antitumor activity of epacadostat (INCB024360), a potent and selective inhibitor of IDO1 PMID: 28053021
    19. Fumaric acid esters inhibit both IDO expression and enzymatic activity leading to a modulation of tryptophan degradation. PMID: 27376248
    20. Our results highlight the role of COX-2 in constitutive IDO1 expression by human tumors and substantiate the use of COX-2 inhibitors to improve the efficacy of cancer immunotherapy, by reducing constitutive IDO1 expression, which contributes to the lack of T-cell infiltration in "cold" tumors, which fail to respond to immunotherapy PMID: 28765120
    21. this data we have identified the structure of two possible compounds that may be even more potent pharmacological repressors of IDO-1. PMID: 28735627
    22. Prognostic value of IDO in acute myeloid leukemia. PMID: 26762931
    23. These findings indicate that IDO1 has the potential to participate in or contribute to the formation of new capillaries, supporting the applicability of IDO1-targeting molecular therapy in lung cancer. PMID: 28498425
    24. induces PD-L1 expression by melanoma cells PMID: 27121174
    25. PD-L1, IDO-1, and B7-H4 are differentially expressed in human lung carcinomas and show limited co-expression. While PD-L1 and IDO-1 are associated with increased tumor-infiltrating lymphoycte and IFN-gamma stimulation, B7-H4 is not. PMID: 27440266
    26. Data show that indole 23-dioxygenase (IDO) is variably expressed by tumor-infiltrating immune cells or reactive cells rather than lymphoma cells in diffuse large-cell lymphoma (DLBCL). PMID: 26727587
    27. High IDO1 expression is associated with hepatocellular carcinoma. PMID: 26895379
    28. Epacadostat significantly decreases Treg proliferation induced by IDO production from IFN-gamma plus LPS matured human DCs, although the Treg phenotype does not change PMID: 27192116
    29. Inhibition of TOR serine-threonine kinases (mTOR) strongly induced indoleamine 23-dioxygenase 1 (IDO1) expression and activity, corroborating its ability to recruit Treg cells in the tumor microenvironment. PMID: 27174915
    30. up-regulation induces immunosuppression and may enhance the risk of hepatitis C virus and hepatitis B virus induced hepatocellular carcinoma [review] PMID: 28428708
    31. MALAT1-overexpressed MSCs promoted M2 macrophage polarization and this effect was mediated by MALAT1-induced IDO expression, suggesting that MALAT1 may enhance the immunosuppressive properties of MSCs in vivo. PMID: 28176360
    32. this study demonstrated that the downregulation of IDO expression on the endothelial cells of the villous stroma was associated with preeclampsia PMID: 28131097
    33. The data suggest that in Puumala infection, the mechanism responsible for the suppressive effect of IDO is not metabolic control of effector cells but rather the signaling mediated by tryptophan breakdown products, such as kynurenine. PMID: 28057727
    34. These results show IDO is upregulated with RSV infection and this upregulation likely participates with IFN-gamma in inhibition of virus replication and suppression of some host cell responses to infection. PMID: 28963880
    35. IDO role in cancer immune responses [review] PMID: 26517538
    36. This study demonstrated that IDO-1 was elevated in the Solid Component and Cyst Fluid of Human Adamantinomatous Craniopharyngioma. PMID: 28859336
    37. The Binding Mode of N-Hydroxyamidines to Indoleamine 2,3-Dioxygenase 1 PMID: 28731684
    38. Fourier transform infrared (FTIR) and nanosecond time-resolved optical spectroscopy to hIDO1 variants with modified heme pocket structures to identify important amino acid residues that stabilize the substrate in the active site. PMID: 28189796
    39. Thus, our study provides novel insights into the interaction between a small molecule inhibitor INCB14943 and IDO1 protein. The structural information will facilitate future IDO1 inhibitor design. PMID: 28412361
    40. The aim of the present study was to clarify the effect of IDO1induced macrophages on the growth of endometrial stromal cells in endometriosis. IDO1 educated-macrophages may facilitate the survival of retrograde endometrial tissues, and be involved in the pathogenesis of endometriosis. PMID: 28260094
    41. Our results identified FGL2, GAL, SEMA4D, SEMA7A, and IDO1 as new candidate genes that could be involved in MSCs-mediated immunomodulation. FGL2, GAL, SEMA4D, SEMA7A, and IDO1 genes appeared to be differentially transcribed in the different MSC populations. Moreover, these genes were not similarly modulated following MSCs-exposure to inflammatory signals PMID: 28336906
    42. IDO mediated conversion of FOXP3 -T cells to Tregs predominantly occurs in children with inflammatory bowel disease. PMID: 28337881
    43. The study confirmed that high IDO expression in pancreatic adenocarcinoma was related to poor prognosis of patients. These findings provided evidence that IDO was involved in pancreatic adenocarcinoma progression and might serve as a relevant therapeutic target. PMID: 28303855
    44. ver-expression of CTLA4 and IDO1 was significantly associated with biochemical recurrence. Our results provide clues on the mechanisms of tumor development and point to potential biomarkers for early detection and treatment for prostate cancer in young men. PMID: 28027300
    45. IDO1 mRNA expression in cervical cancer PMID: 27761872
    46. Structural Study of a Flexible Active Site Loop in Human Indoleamine 2,3-Dioxygenase and Its Functional Implications. PMID: 27112409
    47. IDO gene expression is a feature of aggressive non-muscle-invasive urothelial cell bladder carcinoma, suggesting a potential immunosuppressive role of IDO PMID: 28314306
    48. Data show that in the absence of indoleamine 2,3-dioxygenase (IDO) inhibition, fatty acid oxidation increased along with increased activity of carnitine palmitoyltransferase I (CPT1). PMID: 27667153
    49. High IDO1 expression is associated with cervical cancer. PMID: 27106797
    50. PSG stimulated IDO activity under the conditions of induction of the monocytes by interferon-gamma. PMID: 27595833

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  • 亚细胞定位：
    Cytoplasm, cytosol.
  • 蛋白家族：
    Indoleamine 2,3-dioxygenase family
  • 组织特异性：
    Expressed in mature dendritic cells located in lymphoid organs (including lymph nodes, spleen, tonsils, Peyers's patches, the gut lamina propria, and the thymic medulla), in some epithelial cells of the female genital tract, as well as in endothelial cell
  • 数据库链接：

    HGNC: 6059

    OMIM: 147435

    KEGG: hsa:3620

    STRING: 9606.ENSP00000430505

    UniGene: Hs.840