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  4. Mouse Albumin (Alb)ELISA Kit

Mouse Albumin (Alb)ELISA Kit

  • 中文名称：
    小鼠白蛋白酶联免疫试剂盒
  • 货号：
    CSB-E13878m
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：
        白蛋白（Albumin）是一种由肝脏合成的血浆蛋白，在血液中具有极高的丰度和稳定性。它的分子量约为66 kDa，主要功能是维持血浆渗透压和血容量，以及参与药物、激素和其他生物分子的运输。此外，白蛋白还具有抗氧化、免疫调节、组织修复和再生等功能。由于其多样的生物学功能和广泛的应用价值，白蛋白已经成为医学和生物科学领域的重要研究对象。
        j9九游会登录入口首页生物所提供的小鼠白蛋白(Alb)ELISA Kit属于ELISA检测试剂盒，采用竞争法定量检测鼠血清、血浆、组织匀浆样本中的ALB，其灵敏度为0.110 μg/mL，检测范围为0.781 μg/mL-200 μg/mL。
     
  • 别名：
    Alb 1 ELISA Kit; alb ELISA Kit; ALBU_MOUSE ELISA Kit; Albumin 1 ELISA Kit; Albumin ELISA Kit; Serum albumin ELISA Kit
  • 缩写：
  • Uniprot No.：
  • 种属：
    Mus musculus (Mouse)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    0.781 μg/mL-200 μg/mL
  • 灵敏度：
    0.110 μg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Metabolism
  • 测定原理：
    quantitative
  • 测定方法：
    Competitive
  • 精密度：

     Intra-assay Precision (Precision within an assay): CV%<8%

    Three samples of known concentration were tested twenty times on one plate to assess.

    Inter-assay Precision (Precision between assays):CV%<10%

    Three samples of known concentration were tested in twenty assays to assess. 

  • 线性度：

     To assess the linearity of the assay, samples were spiked with high concentrations of mouse albumin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

  • 标准曲线：

     These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

  • 本试剂盒所含材料：
      • A micro ELISA plate --- The 96-well plate has been pre-coated with the anti-mouse Alb antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
      • Two vials lyophilized standard --- Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
      • One vial Biotin-labeled Alb (100 x concentrate) (60 μl/bottle)
      • One vial HRP-avidin (100 x concentrate) (120 μl/bottle) --- Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
      • One vial Biotin-antibody Diluent (20 ml/bottle) ---Dilute the Biotin-antibody.
      • One vial HRP-avidin Diluent (20 ml/bottle) ---Dilute the HRP-avidin solution.
      • Two vial Sample Diluent (20 ml/bottle)---Dilute the sample to an appropriate concentration.
      • One vial Wash Buffer (25 x concentrate) (20 ml/bottle) --- Wash away unbound or free substances.
      • One vial TMB Substrate (10 ml/bottle) --- Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
      • One vial Stop Solution (10 ml/bottle) --- Stop the color reaction. The solution color immediately turns from blue to yellow.
      • Four Adhesive Strips (For 96 wells) --- Cover the microplate when incubation.
      • An instruction manual

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  • 本试剂盒不含材料：
      • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
      • An incubator that can provide stable incubation conditions up to 37°C±5°C.
      • Centrifuge
      • Vortex
      • Squirt bottle, manifold dispenser, or automated microplate washer
      • Absorbent paper for blotting the microtiter plate
      • 50-300ul multi-channel micropipette
      • Pipette tips
      • Single-channel micropipette with different ranges
      • 100ml and 500ml graduated cylinders
      • Deionized or distilled water
      • Timer
      • Test tubes for dilution

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  • 数据处理：
  • 货期：
    3-5 working days

引用文献

产品评价

平均分:
5.0分 - 1 个评价

样品类型：组织匀浆液 大鼠视网膜组织匀浆中的TNF-α

样品信息：小鼠

稀释比：其他 1:1000

产品评价： 我是实验小白，打算做模式动物的研究，当时筛选了好几个指标，对于这种情况，不太清楚该用什么盒子。因此咨询了他们技术人员，他们工作人员从反应机理、作用途径、反馈情况帮我查阅相关资料，相互交流，最终确定了合适的产品。我使用了CSB-E11987r检测了处理前后大鼠视网膜组织匀浆中TNF-α的水平含量，标曲拟合度良好，操作简单快捷灵敏度高，能助力我顺利完成相关的实验数据的收集。

By 杨老师

靶点详情

  • 最新研究进展：
        白蛋白（ALB）是一种常见的血浆蛋白，具有多种生理功能，包括维持血容量、调节血液渗透压和输送物质等。以下是关于ALB最新的研究进展：
        COVID-19：研究人员发现COVID-19患者血清中ALB水平下降，与严重程度呈正相关。低ALB水平与患者预后不良相关，提示ALB作为COVID-19预后的生物标志物具有潜在价值。
        肾病：ALB是肾病患者中最常见的蛋白质丢失之一。研究表明，ALB水平的下降可能导致肾脏损伤和慢性肾脏病的进展。因此，ALB已成为评估肾脏健康的重要指标之一。
        癌症：一些研究表明，ALB水平下降与肿瘤发生和恶化有关。低ALB水平与预后不良相关，因此ALB可能是肿瘤预后评估的潜在生物标志物之一。
        营养：研究表明，ALB水平可以用作营养状况的指标。ALB水平下降通常与营养不良相关，因此，ALB水平可能有助于评估患者的营养状况。
        总之，最新的研究表明，ALB在许多不同疾病中都发挥着重要的生物学作用，并可能成为评估患者预后和健康状况的重要指标之一。
  • 功能：
    Binds water, Ca(2+), Na(+), K(+), fatty acids, hormones, bilirubin and drugs. Its main function is the regulation of the colloidal osmotic pressure of blood. Major zinc transporter in plasma, typically binds about 80% of all plasma zinc. Major calcium and magnesium transporter in plasma, binds approximately 45% of circulating calcium and magnesium in plasma. Potentially has more than two calcium-binding sites and might additionally bind calcium in a non-specific manner. The shared binding site between zinc and calcium at residue Asp-273 suggests a crosstalk between zinc and calcium transport in the blood. The rank order of affinity is zinc > calcium > magnesium. Binds to the bacterial siderophore enterobactin and inhibits enterobactin-mediated iron uptake of E.coli from ferric transferrin, and may thereby limit the utilization of iron and growth of enteric bacteria such as E.coli. Does not prevent iron uptake by the bacterial siderophore aerobactin.
  • 基因功能参考文献：
    1. findings suggest that HMGB1 induces the transcytosis of albumin via RAGE-dependent Src phosphorylation and Cav-1 phosphorylation. These studies revealed a new mechanism of HMGB1-induced endothelial hyperpermeability. PMID: 27572515
    2. Urinary L-FABP, NGAL, Kim-1 and albumin levels increased during the acute phase of kidney injury and were significantly correlated with the degree of tubulointerstitial fibrosis during the chronic phase. These markers could detect higher risk of progression to CKD. PMID: 27028054
    3. Extending serum half-life of albumin by engineering neonatal Fc receptor (FcRn) binding. PMID: 24652290
    4. We provide evidence of a transcytosis within the kidney tubular system that protects albumin and IgG from lysosomal degradation, allowing these proteins to be recycled intact. PMID: 23970123
    5. albumin may play a distinct role in adipocyte differentiation by promoting lipid accumulation. PMID: 20529675
    6. No binding of albumin was observed at physiological pH to neonatal Fc receptor. At acidic pH, a 100-fold difference in binding affinity was observed. PMID: 20018855
    7. Cellular oxidant stress and advanced glycation endproducts of albumin: caveats of the dichlorofluorescein assay PMID: 11913966
    8. albumin enhancer/promoter was succesfully used for trasient transfection of fluorescent gene reporters (for cell sorting) in ES spontaneously differentiated into hepatocytes. PMID: 18942772

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  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    ALB/AFP/VDB family
  • 组织特异性：
    Plasma. Expressed in the granular cells within the cerebellum.
  • 数据库链接：