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  4. Mouse N-acetyl-β-D-glucosaminidase,NAG ELISA Kit

Mouse N-acetyl-β-D-glucosaminidase,NAG ELISA Kit

  • 中文名称：
    小鼠N-乙酰-β-D-氨基葡萄糖苷酶(NAG)酶联免疫试剂盒
  • 货号：
    CSB-E07444m
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Mouse NAG ELISA Kit was designed for the quantitative measurement of Mouse NAG protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 15.6 μIU/mL-1000 μIU/mL and the sensitivity is 3.9 μIU/mL.

  • 别名：
    Hexb ELISA Kit; Beta-hexosaminidase subunit beta ELISA Kit; EC 3.2.1.52 ELISA Kit; Beta-N-acetylhexosaminidase subunit beta ELISA Kit; Hexosaminidase subunit B ELISA Kit; N-acetyl-beta-glucosaminidase subunit beta ELISA Kit
  • 缩写：
    NAG
  • Uniprot No.：
  • 种属：
    Mus musculus (Mouse)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    15.6 μIU/mL-1000 μIU/mL
  • 灵敏度：
    3.9 μIU/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Others
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse NAG in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:10 Average % 93  
    Range % 85-99  
    1:20 Average % 95  
    Range % 92-98  
    1:40 Average % 90  
    Range % 87-100  
    1:80 Average % 88  
    Range % 86-98  
  • 回收率：
    The recovery of mouse NAG spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 97 86-101  
    EDTA plasma (n=4) 95 90-98  
                 
                 
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    μIU/ml OD1 OD2 Average Corrected  
    1000 2.555 2.529 2.542 2.398  
    500 2.084 2.105 2.095 1.951  
    250 1.442 1.467 1.455 1.311  
    125 0.946 0.955 0.951 0.807  
    62.5 0.413 0.408 0.411 0.267  
    31.2 0.321 0.326 0.324 0.180  
    15.6 0.244 0.239 0.242 0.098  
    0 0.142 0.145 0.144    
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Hydrolyzes the non-reducing end N-acetyl-D-hexosamine and/or sulfated N-acetyl-D-hexosamine of glycoconjugates, such as the oligosaccharide moieties from proteins and neutral glycolipids, or from certain mucopolysaccharides. The isozyme B does not hydrolyze each of these substrates, however hydrolyzes efficiently neutral oligosaccharide. Only the isozyme A is responsible for the degradation of GM2 gangliosides in the presence of GM2A. During fertilization is responsible, at least in part, for the zona block to polyspermy. Present in the cortical granules of non-activated oocytes, is exocytosed during the cortical reaction in response to oocyte activation and inactivates the sperm galactosyltransferase-binding site, accounting for the block in sperm binding to the zona pellucida.
  • 基因功能参考文献：
    1. mast cell granule beta-hexosaminidase is crucial for defense against bacterial invasion, but is not involved in the allergic response. the bactericidal mechanism of beta-hexosaminidase involves degradation of bacterial cell wall peptidoglycan. PMID: 25015817
    2. transgenic inducible strains of Sandhoff disease mice provide a dynamic platform with which to explore the pathophysiological sequelae immediately after loss of neuronal lysosomal beta-hexosaminidase activity. PMID: 23028353
    3. Thymic involution and corticosterone level in Sandhoff disease model mice. PMID: 21598013
    4. These data suggest that the restricted ketogenic diet and N-butyldeoxynojirimycin may be a potential combinatorial therapy for Sandhoff disease. PMID: 20374428
    5. Data suggest that pathogenesis of Sandhoff disease (heritable beta-hexosaminidase deficiency) involves an increase in macrophage-inflammatory protein 1alpha that induces monocytes to infiltrate the CNS and trigger neuronal apoptosis. PMID: 15155903
    6. The bicistronic beta-hexosaminidase vector can reverse the biochemical defects and down-stream consequences in Sandhoff neurons, reinforcing its potential for Sandhoff disease in vivo gene therapy. PMID: 16441513
    7. Beta-hexosaminidase is a peptidoglycan hydrolase that surprisingly exerts its mycobactericidal effect at the macrophage plasma membrane during mycobacteria-induced secretion of lysosomes PMID: 18180457
    8. There was no change in the level of GM2 storage and pro-apoptotic activity or astrocyte activation in HexB-/- knockout mice PMID: 18657867

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  • 亚细胞定位：
    Lysosome. Cytoplasmic vesicle, secretory vesicle, Cortical granule.
  • 蛋白家族：
    Glycosyl hydrolase 20 family
  • 数据库链接：