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Mouse Retinoic Acid ELISA kit

  • 中文名称：
    小鼠视黄酸酶联免疫试剂盒
  • 货号：
    CSB-EQ028019MO
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

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  • 产品描述：

    This Mouse Retinoic Acid ELISA Kit was designed for the quantitative measurement of Mouse Retinoic Acid protein in serum, plasma. It is a Competitive ELISA kit, its detection range is 25 pg/mL-400 pg/mL and the sensitivity is 12.5 pg/mL.

  • 缩写：
    Retinoic Acid
  • 种属：
    Mus musculus (Mouse)
  • 样本类型：
    serum, plasma
  • 检测范围：
    25 pg/mL-400 pg/mL
  • 灵敏度：
    12.5 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Epigenetics and Nuclear Signaling
  • 测定原理：
    quantitative
  • 测定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse retinoic acid in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:20 Average % 92  
    Range % 87-97  
    1:40 Average % 90  
    Range % 85-94  
    1:80 Average % 84  
    Range % 80-88  
    1:160 Average % 89  
    Range % 84-93  
  • 回收率：
    The recovery of mouse retinoic acid spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 98 92-103  
    EDTA plasma (n=4) 94 90-99  
                 
                 
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average    
    400 0.113 0.110 0.112    
    200 0.257 0.246 0.252    
    100 0.451 0.437 0.444    
    50 0.709 0.726 0.718    
    25 1.103 1.081 1.092    
    0 1.672 1.629 1.651    
  • 本试剂盒所含材料：
    • An assay plate --- The 96-well plate has been pre-coated with the anti-mouse retinoic acid antibody.
    • Lyophilized standard (1 x 200 μl) --- Reconstitute the standard with sample diluent at dilution series and draw the standard curve.
    • HRP-conjugate retinoic acid (100 x concentrate) (1 x 60 μl)--- HRP catalyzes the TMB substrate for color development and needs to be diluted before use.
    • HRP-avidin Diluent (1 x 10 ml) --- Dilute the HRP-avidin.
    • Sample Diluent (2 x 20 ml) --- Reconstitute the standard and dilute the sample to an appropriate concentration.
    • Wash Buffer (25 x concentrate) (1 x 20 ml) --- Wash away the unbound solution and non-specific substances.
    • TMB Substrate --- TMB interacts with HRP, eliciting the solution turns blue.
    • Stop Solution (1 x 10 ml) --- Add stop solution and stop the color development. The solution color immediately turns from blue to yellow.
    • Four Adhesive Strips (For 96 wells) --- Cover the microtiter plate when incubation.
    • An instruction manual

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  • 本试剂盒不含材料：
    • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
    • An incubator that can provide stable incubation conditions up to 37°C±5°C.
    • Centrifuge
    • Vortex
    • Squirt bottle, manifold dispenser, or automated microplate washer
    • Absorbent paper for blotting the microtiter plate
    • 50-300ul multi-channel micropipette
    • Pipette tips
    • Single-channel micropipette with different ranges
    • 100ml and 500ml graduated cylinders
    • Deionized or distilled water
    • Timer
    • Test tubes for dilution

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  • 数据处理：
  • 货期：
    3-5 working days

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