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Rabbit Interleukin 6,IL-6 ELISA Kit

  • 中文名称：
    兔子白介素6(IL-6)酶联免疫试剂盒
  • 货号：
    CSB-E06903Rb
  • 规格：
    96T/48T
  • 价格：
    ￥3800/￥2500
  • 其他：

产品详情

  • 产品描述：
        IL6（Interleukin-6），是一种细胞因子，能够影响多种免疫反应、代谢调节以及细胞增殖等生物学过程。IL6在炎症反应中发挥着重要作用，能够吸引中性粒细胞、单核细胞等炎性细胞浸润到炎症部位，同时还能刺激肝脏合成急性反应蛋白等。人体内IL6的含量与炎症反应、肿瘤等疾病的发生密切相关。
        j9九游会登录入口首页生物所提供的Rabbit Interleukin 6,IL-6 ELISA Kit属于ELISA检测试剂盒，采用双抗夹心法定量检测兔血清、血浆、组织匀浆样本中的IL6，其灵敏度为3.9 pg/mL，检测范围为15.6 pg/mL-1000 pg/mL。
     
  • 别名：
    IL6Interleukin-6 ELISA Kit; IL-6 ELISA Kit
  • 缩写：
  • Uniprot No.：
  • 种属：
    Oryctolagus cuniculus (Rabbit)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    15.6 pg/mL-1000 pg/mL
  • 灵敏度：
    3.9 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Immunology
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rabbit IL-6 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:1 Average % 88  
    Range % 80-92  
    1:2 Average % 99  
    Range % 91-105  
    1:4 Average % 101  
    Range % 92-110  
    1:8 Average % 93  
    Range % 86-98  
  • 回收率：
    The recovery of rabbit IL-6 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 93 89-98  
    EDTA plasma (n=4) 96 90-100  
                 
                 
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected  
    1000 2.840 2.785 2.813 2.710  
    500 2.316 2.223 2.270 2.167  
    250 1.580 1.567 1.574 1.471  
    125 0.935 0.874 0.905 0.802  
    62.5 0.479 0.462 0.471 0.368  
    31.2 0.266 0.254 0.260 0.157  
    15.6 0.170 0.158 0.164 0.061  
    0 0.107 0.099 0.103 　  
  • 数据处理：
  • 货期：
    3-5 working days

引用文献

产品评价

问答及客户评论

■ 客户评论
  • Tested application: ELISA
    Test sample: Serum, Tissue homogenate (Liver)
    Species: Rabbit
    Review: I tried liver and serum samples treated and untreated rabbits, the idea was to see with concentration was the best to perform real experiments. The kit work better using liver samples.

靶点详情

  • 最新研究进展：
    IL6（白细胞介素-6）是一种由多种细胞分泌的蛋白质，参与调节免疫、发炎反应和代谢等生理过程。近年来的研究发现，IL6除了作为免疫炎症标志物外，还参与调节体重、代谢疾病、神经系统等多种疾病的发生发展。例如，有研究发现IL6在肥胖、糖尿病、帕金森病、阿尔茨海默病等疾病的发生中发挥了重要作用。此外，IL6还可以作为一种治疗性药物，被用于治疗某些炎症性疾病和肿瘤。
  • 功能：
    Cytokine with a wide variety of biological functions in immunity, tissue regeneration, and metabolism. Binds to IL6R, then the complex associates to the signaling subunit IL6ST/gp130 to trigger the intracellular IL6-signaling pathway. The interaction with the membrane-bound IL6R and IL6ST stimulates 'classic signaling', whereas the binding of IL6 and soluble IL6R to IL6ST stimulates 'trans-signaling'. Alternatively, 'cluster signaling' occurs when membrane-bound IL6:IL6R complexes on transmitter cells activate IL6ST receptors on neighboring receiver cells.; IL6 is a potent inducer of the acute phase response. Rapid production of IL6 contributes to host defense during infection and tissue injury, but excessive IL6 synthesis is involved in disease pathology. In the innate immune response, is synthesized by myeloid cells, such as macrophages and dendritic cells, upon recognition of pathogens through toll-like receptors (TLRs) at the site of infection or tissue injury. In the adaptive immune response, is required for the differentiation of B cells into immunoglobulin-secreting cells. Plays a major role in the differentiation of CD4(+) T cell subsets. Essential factor for the development of T follicular helper (Tfh) cells that are required for the induction of germinal-center formation. Required to drive naive CD4(+) T cells to the Th17 lineage. Also required for proliferation of myeloma cells and the survival of plasmablast cells.; Acts as an essential factor in bone homeostasis and on vessels directly or indirectly by induction of VEGF, resulting in increased angiogenesis activity and vascular permeability. Induces, through 'trans-signaling' and synergistically with IL1B and TNF, the production of VEGF. Involved in metabolic controls, is discharged into the bloodstream after muscle contraction increasing lipolysis and improving insulin resistance. 'Trans-signaling' in central nervous system also regulates energy and glucose homeostasis. Mediates, through GLP-1, crosstalk between insulin-sensitive tissues, intestinal L cells and pancreatic islets to adapt to changes in insulin demand. Also acts as a myokine. Plays a protective role during liver injury, being required for maintenance of tissue regeneration. Also has a pivotal role in iron metabolism by regulating HAMP/hepcidin expression upon inflammation or bacterial infection. Through activation of IL6ST-YAP-NOTCH pathway, induces inflammation-induced epithelial regeneration.
  • 基因功能参考文献：
    1. Mild heat shock increased the production of inflammatory cytokines, IL-1beta and IL-6 in rabbit cornea cells. PMID: 23330884
  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    IL-6 superfamily
  • 数据库链接：