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Rat C-Reactive Protein,CRP ELISA Kit

  • 中文名称：
    大鼠C反应蛋白(CRP)酶联免疫试剂盒
  • 货号：
    CSB-E07922r
  • 规格：
    96T/48T
  • 价格：
    ￥3900/￥2500
  • 其他：

产品详情

  • 产品描述：
        CRP，即C-反应蛋白（C-reactive protein），是一种急性相蛋白，主要由肝脏合成，可作为炎症的非特异性生物标志物。当机体遇到感染、损伤、炎症等刺激时，肝脏合成和分泌CRP，其浓度会在数小时内显著升高。因此，CRP常被用于评估感染、炎症和自身免疫性疾病等疾病的活动程度和预后，也可用于评估心血管疾病和癌症等疾病的风险。正常情况下，血浆中CRP的浓度很低，但在炎症反应期可升高数千倍。
        j9九游会登录入口首页生物所提供的Rat C-Reactive Protein,CRP ELISA Kit属于ELISA检测试剂盒，采用双抗夹心法定量检测大鼠血清、血浆、细胞培养上清、组织匀浆、细胞裂解液样本中的CRP，其灵敏度为7.81 ng/mL，检测范围为31.25 ng/mL-2000 ng/mL。
     
  • 别名：
    Crp ELISA kit; Ptx1C-reactive protein ELISA kit
  • 缩写：
  • Uniprot No.：
  • 种属：
    Rattus norvegicus (Rat)
  • 样本类型：
    serum, plasma, cell culture supernates, tissue homogenates, cell lysates
  • 检测范围：
    31.25 ng/mL-2000 ng/mL
  • 灵敏度：
    7.81 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Immunology
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat CRP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:1 Average % 92  
    Range % 88-96  
    1:2 Average % 89  
    Range % 85-96  
    1:4 Average % 93  
    Range % 89-97  
    1:8 Average % 99  
    Range % 92-105  
  • 回收率：
    The recovery of rat CRP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 93 88-97  
    EDTA plasma (n=4) 96 91-99  
                 
                 
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average Corrected  
    2000 2.664 2.701 2.683 2.541  
    1000 1.701 1.716 1.709 1.567  
    500 1.173 1.182 1.178 1.036  
    250 0.604 0.589 0.597 0.455  
    125 0.398 0.372 0.385 0.243  
    62.5 0.302 0.288 0.295 0.153  
    31.25 0.234 0.227 0.231 0.089  
    0 0.144 0.140 0.142 　  
  • 数据处理：
  • 货期：
    3-5 working days

引用文献

产品评价

问答及客户评论

■ 客户评论
  • Sample type: Supernatant
    Sample species: Rat
    Review: Serum concentrations of biomarkers, including TGF-β1, TNFα, IL-1β, IL-6, IL-18, CRP, and BNP were measured by Elisa kits following manufacture instructions. As shown in figure, the levels of TGF-β1, CRP, IL-6 and BNP were significantly increased in MI rats than Sham. Salvianolate treatment dose-dependently reduced these levels compared with MI rats.
    PMID: 30466624
  • Sample type: Serum
    Sample species: Rat
    Review: Renin, ANGII, C-reactive protein (CRP) and interleukin-6 (IL-6) levels were determined by ELISA. Our results advocate a positive correlation between CRP serum levels and CSA, which is indicative for vascular hypertrophy . Irrespective of the induction of hypertension, CRP may be implicated as mediator of vascular remodeling after injury
    PMID: 30021383
  • Sample type: Serum
    Sample species: Rat
    Review: Frozen aliquots of serum samples were thawed firstly on ice. Subsequently, commercial ELISA kits were used to detect the levels of interleukin-6 (IL-6), tumor necrosis factor alfa (TNF-α), C-reactive protein (CRP), adrenocorticotropic hormone (ACTH), corticotropin-releasing hormone (CRH), and corticosterone (CORT) in serum, following the instructions provided by the manufacturer. Chronic PSD for 7 days resulted in higher levels of serum pro-inflammatory cytokines including IL-6, TNF-α, and CRP with P values < 0.001, 0.01 and 0.001, respectively
    PMID: 30953642
  • Sample type: Serum
    Sample species: Rat
    Review: The levels of serum C-reactive protein (CRP) were determined with ELISA kits. Effect of HuoxueAnshen Recipe on serum CRP before and 2 h after I/R.
    PMID: 28479928
  • Sample type: Plasma
    Sample species: Rat
    Review: The levels of serum C-reactive protein (CRP) were determined with ELISA kits. The contents of plasma MDA, TNF-α, and CRP were significantly higher in the moderate and high- dose PM2.5 exposure groups than in the controls (P < .05).
    PMID: 27592525

靶点详情

  • 最新研究进展：
    CRP是C反应蛋白的简称，是一种急性反应蛋白。最新研究表明，CRP不仅可以作为心血管疾病等疾病的生物标志物，还与COVID-19感染的严重程度相关。此外，研究还发现CRP参与多种疾病的发生发展，包括癌症、自身免疫疾病和神经退行性疾病等。
  • 功能：
    Displays several functions associated with host defense: it promotes agglutination, bacterial capsular swelling, phagocytosis and complement fixation through its calcium-dependent binding to phosphorylcholine. Can interact with DNA and histones and may scavenge nuclear material released from damaged circulating cells.
  • 基因功能参考文献：
    1. coordinated upregulation of genes encoding CRP, interleukin-6 (IL-6), HNF1alpha, and HNF4alpha in the livers and white adipose tissue (WAT) of chronic renal failure rats, as compared to the pair-fed and control animals. PMID: 29330688
    2. CRP: varied between DJOS and SHAM groups maintained on HF/HF, CD/CD, and CD/HF. PMID: 29849871
    3. Fibrinopeptide A induces CRP expression in Vascular Smooth Muscle Cells via Reactive Oxygen Species-ERK1/2/p38-NF-kappaB signal pathway. PMID: 29768263
    4. Knockdown of ZFAS1 or miR-150 overexpression effectively relieved AMI-induced myocardial infarction in AMI-1 week rats. The ZFAS1/miR-150 axis was involved in the molecular mechanism of AMI induced cardiomyocytes apoptosis via regulating CRP PMID: 28295592
    5. Epigallocatechin-3-gallate inhibits Ang II-induced CRP generation by interfering with AT1-ROS-ERK1/2 signal pathway in hepatocytes, preventing inflammation. PMID: 27492718
    6. Salvianolic acid B exerted anti-steatotic and anti-inflammatory effects against alcoholic liver injury by inducing SIRT1-mediated inhibition of CRP and ChREBP expression. PMID: 27989594
    7. Rosiglitazone inhibits angiotensin II-induced C-reactive protein generation by interfering with AT1-ROS-ERK1/2/JNK signal pathway and up-regulating PPARgamma expression in hepatocytes PMID: 26741265
    8. These findings provide evidence that, in the presence of high CRP levels, rosuvastatin exhibits significant anti-inflammatory, anti-oxidative, and renoprotective effects. PMID: 25536316
    9. Data show that palmitic acid significantly stimulated mRNA and protein expression of C-reactive protein, inducible nitric oxide synthase and tumor necrosis factor-alpha in vascular smooth muscle cells in time- and concentration-dependent manners. PMID: 25242580
    10. No correlation was found between CRP levels and microvascular or macrovascular dysfunction in adjuvant-induced arthritis. PMID: 25708190
    11. Homocysteine initiates an inflammatory response in VSMCs by stimulating CRP production via the NMDAr-ROS-ERK1/2/p38-NF-kappaB signal pathway. PMID: 25016361
    12. CRP and IgM depositions demonstrate a parallel time course throughout the reperfusion to hepatocellular damage, inflammatory response and activated complement deposition in an ischemia-reperfusion model. PMID: 24642533
    13. These demonstrate that aldosterone is able to induce CRP expression via MR-ROS-ERK1/2-NF-kappaB signal pathway in VSMCs, which provides a new evidence for the proinflammatory and proatherosclerotic effects of aldosterone. PMID: 25109280
    14. Monomeric CRP localized with inflammatory cells in infarcted myocardium. Intravenous pCRP administration significantly enhanced leukocyte rolling, adhesion, and transmigration via localized dissociation to mCRP in inflamed cremaster muscle. PMID: 24982116
    15. In older animals, there is depressed PAP expression related to a blunted inflammatory response in acute pancreatitis which is associated with worsened bacterial infiltration and higher C-reactive protein level PMID: 22807607
    16. PDIA3 might be potentially involve into the occurence and development of acute rejection response in renal transplantation and increased plasma IL-2, IL-4, IL-6, and CRP levels play an important role to prevent acute kidney allograft rejection in rats. PMID: 22201020
    17. C-reactive protein down-regulates endothelial nitric oxide synthase expression and promotes apoptosis in endothelial progenitor cells through receptor for advanced glycation end-products PMID: 22301267
    18. After attachment of polymeric CRP to the surface of necrotic cardiomyocytes, generation of monomeric CRP was demonstrated over a period of 18 h. PMID: 21856781
    19. This finding could indicate a possible role for CRP in the progression of small vessel disease in the brain and provide a therapeutic target PMID: 21840509
    20. C-reactive protein and tumour necrosis factor-alpha are elevated in collagen-induced arthritis in Dark Agouti rats PMID: 21633874
    21. Increased levels of CRP are an early sign of alcoholic fatty liver in rats. PMID: 21806828
    22. C-reactive protein exerts a proinflammatory action via Toll-like receptor 4-dependent signaling pathway (AT(1)R-p38 MAPK-TLR4-PKCalpha) in rat vascular smooth muscle cells. PMID: 20332628
    23. CRP may cause insulin resistance by increasing IRS-1 phosphorylation at Ser(307) and Ser(612) via JNK and ERK1/2, respectively, leading to impaired insulin-stimulated glucose uptake, GLUT4 translocation, and glycogen synthesis PMID: 17279354
    24. the Ala12 allele of PPARG2 may be an independent factor associated with low serum hs-CRP levels in Japanese females PMID: 17574226
    25. levels of C-reactive protein and adiponectin are improved after exercise training in diabetic rats PMID: 17660720
    26. CRP and IgM depositions correlated well with local complement activation, which suggests a role of these molecules in complement activation PMID: 17981193
    27. CRP levels and cardiovascular disease risk and the long term clinical outcome after acute coronary syndrome but such correlation has yet to be proven regarding CRP and atherosclerosis. PMID: 18254451
    28. C-reactive protein stimulates superoxide anion release and tissue factor activity in vivo PMID: 18621373
    29. CRP at clinically relevant concentrations (10 to 20 microg/mL) causes a disruption of the blood-brain barrier PMID: 19246692
    30. The results suggest that CRP contributes to memory loss and early phase of pathogenesis of Alzheimer disease PMID: 19263040
    31. The results demonstrate that ET-1 induces CPR production in VSMCs via ET(A) receptor followed by ROS and MAPK signal pathway. PMID: 19748488

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  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    Pentraxin family
  • 组织特异性：
    Found in plasma.
  • 数据库链接：