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  4. Rat Podocalyxin,PCX ELISA Kit

Rat Podocalyxin,PCX ELISA Kit

  • 中文名称：
    大鼠足细胞标记蛋白/足盂蛋白(PCX)酶联免疫试剂盒
  • 货号：
    CSB-E13946r
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    CUSABIO's rat podocalyxin (PCX) ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of rat PCX in serum, urine, or cell culture supernates. This assay uses the sandwich enzyme immunoassay technique in combination with the enzyme-substrate chromogenic reaction to quantify the analyte in the sample. The color develops positively to the amount of PCX in samples. The color intensity is measured at 450 nm via a microplate reader.

    PCX, also called PODXL, is a type I membrane mucoprotein abundantly presented in the podocytes of kidney glomeruli where it plays an important role in maintaining the structural organization of the kidney glomeruli and normal plasma filtration. Dominant and recessive mutations in PODXL are linked to human kidney disease. Abnormal expression of PODXL has been implicated in a range of cancers. PODXL is upregulated on tumors with high metastatic index. The presence of PODXL is associated with poor outcomes, thus emerging as an important prognostic and theragnostic marker in several human cancers.

  • 别名：
    Podxl ELISA Kit; Pclp1 ELISA Kit; Podocalyxin ELISA Kit; Podocalyxin-like protein 1 ELISA Kit; PC ELISA Kit; PCLP-1 ELISA Kit
  • 缩写：
  • Uniprot No.：
  • 种属：
    Rattus norvegicus (Rat)
  • 样本类型：
    serum, urine, cell culture supernates
  • 检测范围：
    1.56 ng/mL-100 ng/mL
  • 灵敏度：
    0.39 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Cancer
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat PCX in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:1Average %87
    Range %81-93
    1:2Average %97
    Range %93-101
    1:4Average %89
    Range %85-93
    1:8Average %98
    Range %92-104
  • 回收率：
    The recovery of rat PCX spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9185-97
    EDTA plasma (n=4)8983-94
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    1002.201 2.312 2.257 2.112
    501.574 1.456 1.515 1.370
    251.009 1.056 1.033 0.888
    12.50.633 0.654 0.644 0.499
    6.250.401 0.412 0.407 0.262
    3.120.272 0.286 0.279 0.134
    1.560.210 0.215 0.213 0.068
    00.144 0.146 0.145
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Involved in the regulation of both adhesion and cell morphology and cancer progression. Functions as an anti-adhesive molecule that maintains an open filtration pathway between neighboring foot processes in the podocyte by charge repulsion. Acts as a pro-adhesive molecule, enhancing the adherence of cells to immobilized ligands, increasing the rate of migration and cell-cell contacts in an integrin-dependent manner. Induces the formation of apical actin-dependent microvilli. Involved in the formation of a preapical plasma membrane subdomain to set up initial epithelial polarization and the apical lumen formation during renal tubulogenesis. Plays a role in cancer development and aggressiveness by inducing cell migration and invasion through its interaction with the actin-binding protein EZR. Affects EZR-dependent signaling events, leading to increased activities of the MAPK and PI3K pathways in cancer cells.
  • 基因功能参考文献：
    1. podocalyxin has a role in defining the structure of the podocyte basal surface PMID: 15226400
    2. Podocalyxin might be associated with the malignant progression of astrocytic tumors, and be a useful prognostic marker for astrocytic tumors. PMID: 18639524
    3. Report ubiquitous yet distinct expression of podocalyxin on vascular surfaces in normal and tumor tissues in the rat. PMID: 19142011
  • 亚细胞定位：
    Apical cell membrane. Cell projection, microvillus. Membrane raft. Cell projection, lamellipodium. Cell projection, filopodium. Cell projection, ruffle. Membrane; Single-pass type I membrane protein.
  • 蛋白家族：
    Podocalyxin family
  • 组织特异性：
    Glomerular epithelium cell (podocyte) (at protein level).
  • 数据库链接：