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  4. Rat Troponin I, slow skeletal muscle(TNNI1) ELISA kit

Rat Troponin I, slow skeletal muscle(TNNI1) ELISA kit

  • 中文名称：
    大鼠肌钙蛋白I，骨骼肌慢肌(TNNI1)酶联免疫试剂盒
  • 货号：
    CSB-EL024011RA
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Rat TNNI1 ELISA Kit was designed for the quantitative measurement of Rat TNNI1 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 15.6 pg/mL-1000 pg/mL and the sensitivity is 3.9 pg/mL.

  • 别名：
    Tnni1 ELISA Kit; Troponin I ELISA Kit; slow skeletal muscle ELISA Kit; Troponin I ELISA Kit; slow-twitch isoform ELISA Kit
  • 缩写：
    TNNI1
  • Uniprot No.：
  • 种属：
    Rattus norvegicus (Rat)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    15.6 pg/mL-1000 pg/mL
  • 灵敏度：
    3.9 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Signal Transduction
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%        
    Three samples of known concentration were tested twenty times on one plate to assess.    
    Inter-assay Precision (Precision between assays): CV%<10%        
    Three samples of known concentration were tested in twenty assays to assess.      
                   
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat TNNI1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
    　 Sample Serum(n=4)    
    1:1 Average % 84    
    Range % 80-88    
    1:2 Average % 90    
    Range % 86-94    
    1:4 Average % 95    
    Range % 90-100    
    1:8 Average % 92    
    Range % 88-96    
  • 回收率：
    The recovery of rat TNNI1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
     
    Sample Type Average % Recovery Range    
    Serum (n=5) 88 84-92    
    EDTA plasma (n=4) 103 99-108    
                   
                   
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
     
    pg/ml OD1 OD2 Average Corrected    
    1000 2.526 2.423 2.475 2.289    
    500 1.875 1.756 1.816 1.630    
    250 1.212 1.159 1.186 1.000    
    125 0.784 0.811 0.798 0.612    
    62.5 0.500 0.490 0.495 0.309    
    31.2 0.347 0.362 0.355 0.169    
    15.6 0.291 0.274 0.283 0.097    
    0 0.183 0.188 0.186 　    
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Troponin I is the inhibitory subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.
  • 基因功能参考文献：
    1. these results suggest that cTnI(R146G) and cTnI(R21C) blunt PKA modulation of activation and relaxation kinetics by prohibiting cardiac-specific N-terminal interaction with the cTnI inhibitory peptide. PMID: 26391394
    2. troponin I phosphorylation has a role in protein kinase C-mediated enhanced contractile performance of myocytes PMID: 12815045
    3. A novel role is described for cardiac TnI PKA phosphorylation in the rate-dependent enhancement of systolic & diastolic function in vivo & afterload sensitivity of relaxation. This has implications for cardiac failure. PMID: 14726477
    4. MuRF1 functions as a ubiquitin ligase to catalyze ubiquitylation of troponin I through a RING finger-dependent mechanism PMID: 15601779
    5. TnI-isoform and titin-isoform switching and corresponding functional changes during heart development are not initiated by birth but are genetically programmed PMID: 16679402
    6. The goal here was to identify domain clusters and residues that confer troponin I isoform-specific myofilament Ca2+ and pH sensitivities of contraction. PMID: 17602701
    7. This study highlights cellular contractile defects by cardiomyopathy mutant cTnIs that are allele and species dependent. PMID: 18423659
    8. Study identifies tyrosine phosphorylation as a novel protein kinase Cdelta (PKCdelta) activation mechanism that modifies PKCdelta-dependent phosphorylation of cardiac troponin I. PMID: 18550549
    9. The negative inotropic effect of HIV gp120 is mediated by a novel signaling pathway that begins with binding to a cardiac myocyte CXCR4 receptor, followed by phosphorylation of both p38 MAP kinase and Troponin I. PMID: 18850076
    10. observed higher relative level of natural antisense RNA in neonatal cardiac fibroblasts compared to neonatal cardiomyocytes, pointing to a cell type-specific expression of sense and antisense RNA PMID: 19184367
    11. These findings suggest that altered TnI function, due to changes in the distribution of phosphorylated sites, is an early contributor to reduced contractility of the heart. PMID: 19507043

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  • 蛋白家族：
    Troponin I family
  • 数据库链接：

    KEGG: rno:29388

    STRING: 10116.ENSRNOP00000012096

    UniGene: Rn.4035