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VPS39 Antibody

  • 中文名称：
    VPS39兔多克隆抗体
  • 货号：
    CSB-PA836235LA01HU
  • 规格：
    ￥440
  • 促销：
    小规格抗体限时一口价
  • 图片：
    • Western Blot
      Positive WB detected in: Mouse heart tissue, Mouse kidney tissue
      All lanes: VPS39 antibody at 3μg/ml
      Secondary
      Goat polyclonal to rabbit IgG at 1/50000 dilution
      Predicted band size: 102, 101 kDa
      Observed band size: 102 kDa
    • Immunohistochemistry of paraffin-embedded human liver tissue using CSB-PA836235LA01HU at dilution of 1:100
    • Immunofluorescent analysis of A549 cells using CSB-PA836235LA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
    • Western Blot
      Positive WB detected in: SH-SY5Y whole cell lysate
      All lanes: VPS39 antibody at 1:2000
      Secondary
      Goat polyclonal to rabbit IgG at 1/50000 dilution
      Predicted band size: 102, 101 kDa
      Observed band size: 102 kDa
  • 其他：

产品详情

  • 产品描述：

    The recombinant peptide corresponding to amino acids 263-490 of the human VPS39 is used as the immunogen to immunize the rabbit to yield the anti-VPS39 antibody. The VPS39 polyclonal antibody exists as an unconjugated IgG isoform. Its purity is 95%+ using protein G purified. It reacts with human and mouse VPS39. This VPS39 antibody has been validated for use in ELISA, WB, IF, and IHC analyses.

    VPS39 mainly regulates endosomal membrane trafficking and fusion. It is a subunit of the homotypic fusion and protein sorting (HOPS) complex, which is involved in the tethering and fusion of endosomes and lysosomes. It is also involved in the regulation of lysosomal biogenesis, autophagy, and signaling pathways. Mutations in the VPS39 gene have been linked to various diseases such as Hermansky-Pudlak syndrome.

  • 产品名称：
    Rabbit anti-Homo sapiens (Human) VPS39 Polyclonal antibody
  • Uniprot No.：
    Q96JC1
  • 基因名：
  • 别名：
    A230065P22Rik antibody; AW743070 antibody; FLJ21681 antibody; FLJ46546 antibody; hVam6p antibody; KIAA0770 antibody; RP23-348L16.12 antibody; TLP antibody; TRAP1 like protein antibody; Vacuolar protein sorting 39 antibody; Vacuolar protein sorting 39 homolog antibody; VAM6 antibody; Vam6/Vps39 like antibody; Vam6/Vps39 like protein antibody; Vam6/Vps39-like protein antibody; VPS 39 antibody; Vps39 antibody; VPS39_HUMAN antibody
  • 宿主：
    Rabbit
  • 反应种属：
    Human, Mouse
  • 免疫原：
    Recombinant Human Vam6/Vps39-like protein (263-490AA)
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated

    本页面中的产品，VPS39 Antibody (CSB-PA836235LA01HU)，的标记方式是Non-conjugated。对于VPS39 Antibody，我们还提供其他标记。见下表：

    可提供标记
    标记方式 货号 产品名称 应用
    HRP CSB-PA836235LB01HU VPS39 Antibody, HRP conjugated ELISA
    FITC CSB-PA836235LC01HU VPS39 Antibody, FITC conjugated
    Biotin CSB-PA836235LD01HU VPS39 Antibody, Biotin conjugated ELISA
  • 克隆类型：
    Polyclonal
  • 抗体亚型：
    IgG
  • 纯化方式：
    >95%, Protein G purified
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, WB, IHC, IF
  • 推荐稀释比：
    Application Recommended Dilution
    WB 1:500-1:5000
    IHC 1:20-1:200
    IF 1:50-1:200
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Regulator of TGF-beta/activin signaling, inhibiting SMAD3- and activating SMAD2-dependent transcription. Acts by interfering with SMAD3/SMAD4 complex formation, this would lead to inhibition of SMAD3-dependent transcription and relieve SMAD3 inhibition of SMAD2-dependent promoters, thus increasing SMAD2-dependent transcription. Does not affect TGF-beta-induced SMAD2 or SMAD3 phosphorylation, nor SMAD2/SMAD4 complex formation.; Plays a role in vesicle-mediated protein trafficking to lysosomal compartments including the endocytic membrane transport and autophagic pathways. Acts as a component of the putative HOPS endosomal tethering complex which is proposed to be involved in the Rab5-to-Rab7 endosome conversion probably implicating MON1A/B, and via binding SNAREs and SNARE complexes to mediate tethering and docking events during SNARE-mediated membrane fusion. The HOPS complex is proposed to be recruited to Rab7 on the late endosomal membrane and to regulate late endocytic, phagocytic and autophagic traffic towards lysosomes. Involved in homotypic vesicle fusions between late endosomes and in heterotypic fusions between late endosomes and lysosomes. Required for fusion of endosomes and autophagosomes with lysosomes.
  • 基因功能参考文献：
    1. TLP expression contributes to hypertrophic scar formation and contraction. PMID: 25655281
    2. TLP likely acts as a molecular modulator capable of altering the balance of Smad3- and Smad2-dependent signaling through regulation of phosphorylation, thus facilitating collagen synthesis in fibroblasts. PMID: 23418473
    3. Vps39 knockdown impairs late endosome fusion and fusion between late endosomes and lysosomes. PMID: 23167963
    4. Merkel cell polyomavirus large T antigen disrupts lysosome clustering by translocating human Vam6p from the cytoplasm to the nucleus PMID: 21454559
    5. although only TBC1D15/Rab7-GAP altered Rab7-GTP levels, both Rab7-GAP and mVps39 regulate lysosomal morphology and play a role in maintaining growth factor dependence PMID: 20363736
    6. propose that TLP might regulate the balance of Smad2 and Smad3 signaling by localizing Smad4 intracellularly, thus contributing to cellular specificity of TGF-beta transcriptional responses in both normal and pathophysiology PMID: 12941698

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  • 亚细胞定位：
    Cytoplasm. Lysosome membrane; Peripheral membrane protein. Late endosome membrane; Peripheral membrane protein.;
  • 蛋白家族：
    VAM6/VPS39 family
  • 组织特异性：
    Widely expressed, with highest levels in heart, skeletal muscle, kidney, pancreas, brain, placenta and spleen.
  • 数据库链接：

    HGNC: 20593

    OMIM: 612188

    KEGG: hsa:23339

    STRING: 9606.ENSP00000326534

    UniGene: Hs.88025