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Human Collagen Type Ⅰ,Col Ⅰ ELISA Kit

  • 中文名称:
    人Ⅰ型胶原(Col Ⅰ)酶联免疫试剂盒
  • 货号:
    CSB-E08082h
  • 规格:
    96T/48T
  • 价格:
    ¥3600/¥2500
  • 其他:

产品详情

  • 产品描述:
        Col Ⅰ(Ⅰ型胶原蛋白,Type I Collagen)是一种主要存在于动物体内结缔组织中的胶原蛋白。胶原蛋白是组成细胞外基质的重要结构蛋白,对维持组织的稳定性、弹性和机械强度起着关键作用。Col Ⅰ在人体中含量丰富,主要分布于皮肤、骨骼、肌腱、血管壁等组织中。Col Ⅰ的合成和降解过程在组织修复、生长发育和疾病发生中具有重要生物学意义,对于维持正常组织结构和功能具有重要调控作用。
        j9九游会登录入口首页生物所提供的Human Collagen Type Ⅰ,Col Ⅰ ELISA Kit属于ELISA检测试剂盒,采用双抗夹心法定量检测人血清、尿液、细胞培养上清、组织匀浆样本中的Col Ⅰ,其灵敏度为0.39 ng/mL,检测范围为1.56 ng/mL-100 ng/mL。
     
  • 别名:
    Collagen Type 1,Col 1,_x000D_Collagen Type I,Col I
  • 缩写:
    Col Ⅰ
  • 种属:
    Homo sapiens (Human)
  • 样本类型:
    serum, urine, cell culture supernates, tissue homogenates
  • 检测范围:
    1.56 ng/mL-100 ng/mL
  • 灵敏度:
    0.39 ng/mL
  • 反应时间:
    1-5h
  • 样本体积:
    50-100ul
  • 检测波长:
    450 nm
  • 研究领域:
    Signal Transduction
  • 测定原理:
    quantitative
  • 测定方法:
    Sandwich
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of human Col Ⅰ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:1 Average % 87  
    Range % 80-92  
    1:2 Average % 100  
    Range % 91-110  
    1:4 Average % 105  
    Range % 92-110  
    1:8 Average % 93  
    Range % 86-100  
  • 回收率:
    The recovery of human Col Ⅰ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 94 89-99  
                 
                 
  • 标准曲线:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average Corrected  
    100 2.921 2.985 2.953 2.767  
    50 2.356 2.378 2.367 2.181  
    25 1.826 1.875 1.851 1.665  
    12.5 1.221 1.256 1.239 1.053  
    6.25 0.796 0.800 0.798 0.612  
    3.12 0.512 0.546 0.529 0.343  
    1.56 0.302 0.326 0.314 0.128  
    0 0.188 0.184 0.186    
  • 数据处理:
  • 货期:
    3-5 working days

引用文献

产品评价

平均分:
4.3分 - 3 个评价

样品类型:血清 ,细胞培养上清液

样品信息:人

稀释比:其他 1:10

产品评价: human collagen type1 COL1 ELISA试剂盒检测人血清/血浆,正常、模型和给药组样本数据很稳定,复孔差异较小。血清/血浆结果相近,COL1灵敏度比COL3高,试剂盒稳定方便。

By D老师

样品类型:细胞培养上清液

样品信息:人

稀释比:没有稀释

产品评价: 使用CSB-E08082h检测外泌体促进成纤维细胞一型胶原蛋白的产生,其OD值如附件图。使用过程中说明书很详细,按照步骤做实验很顺利。

By 邢老师

样品类型:细胞培养上清液

样品信息:人

稀释比:其他 1:5

产品评价: 使用CSB-E08082h试剂盒检测细胞中collagen 1含量,标曲趋势正确,数据完好。

By 张老师

靶点详情

  • 最新研究进展:
    Collagen Ⅰ(Ⅰ型胶原)是一种主要存在于结缔组织中的蛋白质,最新研究表明它在疾病发生和治疗中也起着重要作用。例如,肝纤维化和骨质疏松等疾病都与胶原Ⅰ的异常表达有关。此外,一些药物和生物制剂已经用于调节胶原Ⅰ的合成和代谢,以治疗相关疾病。