Mouse Collagen Type Ⅲ,Col Ⅲ ELISA KIT

产品详情

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

This Mouse Col Ⅲ ELISA Kit was designed for the quantitative measurement of Mouse Col Ⅲ protein in serum, plasma. It is a Sandwich ELISA kit, its detection range is 3.12 pg/mL-200 pg/mL and the sensitivity is 0.78 pg/mL.
别名：

Collagen Type 3,Col 3,_x000D_ Collagen Type III,Col III
缩写：

Col Ⅲ
种属：

Mus musculus (Mouse)
样本类型：

serum, plasma
检测范围：

3.12 pg/mL-200 pg/mL
灵敏度：

0.78 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Signal Transduction
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse Col Ⅲ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:20	Average %	89
	Range %	82-96
1:40	Average %	98
	Range %	94-102
1:80	Average %	97
	Range %	90-104
1:160	Average %	86
	Range %	82-90

回收率：

The recovery of mouse Col Ⅲ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	93	87-99
EDTA plasma (n=4)	100	96-104

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.