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Rabbit Complement 3,C3 ELISA Kit

  • 中文名称:
    兔补体蛋白3(C3)酶联免疫试剂盒
  • 货号:
    CSB-E08668Rb
  • 规格:
    96T/48T
  • 价格:
    ¥4200/¥3000
  • 其他:

产品详情

  • 产品描述:

    This Rabbit Complement 3 (C3) ELISA Kit was designed for the quantitative measurement of Rabbit Complement 3 (C3) protein in serum, plasma. It is a Competitive ELISA kit, its detection range is 27.3 ng/mL-7000 ng/mL and the sensitivity is 27.3 ng/mL.

  • 别名:
    AHUS5, ARMD9, ASP, CPAMD1, acylation-stimulating protein cleavage product|complement component C3
  • 缩写:
  • 种属:
    Oryctolagus cuniculus (Rabbit)
  • 样本类型:
    serum, plasma
  • 检测范围:
    27.3 ng/mL-7000 ng/mL
  • 灵敏度:
    27.3 ng/mL
  • 反应时间:
    1-5h
  • 样本体积:
    50-100ul
  • 检测波长:
    450 nm
  • 研究领域:
    Immunology
  • 测定原理:
    quantitative
  • 测定方法:
    Competitive
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of rabbit C3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:1000Average %97
    Range %91-110
    1:2000Average %89
    Range %82-99
    1:4000Average %95
    Range %85-99
    1:8000Average %91
    Range %87-98
  • 回收率:
    The recovery of rabbit C3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9382-97
    EDTA plasma (n=4)9787-100
  • 标准曲线:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2Average
    70000.196 0.189 0.193
    17500.378 0.372 0.375
    437.50.812 0.805 0.809
    109.41.492 1.501 1.497
    27.32.012 2.003 2.008
    02.913 2.897 2.905
  • 数据处理:
  • 货期:
    3-5 working days

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问答及客户评论

 常见问题解答
Q:

What is the standard in the Rabbit Complement 3 ELISA kit?
code CSB-E08668Rb. I want to know if it is actually rabbit complement C3. If you could get me a molecular weight of the standard, that will be helpful. I need to be able to convert between mg/ml and molarity for my assay and to do that I need the molecular weight of the protein. NO sequence needed, just a weight in kD.

A:
Thanks for your inquiry.
CSB-E08668Rb
Standard is purified crude extract from native samples.
We don't suggest you convert the unit. Pls kindly follow the unit in the manual.
If you want to convert it, you can refer to: https://www.uniprot.org/uniprot/P12247.
Pls let me know if you have any further questions. Thank you.