Your Good Partner in Biology Research

  1. Home
  2. 抗体
  3. 多克隆抗体
  4. ANO2 Antibody

ANO2 Antibody

  • 中文名称：
    ANO2兔多克隆抗体
  • 货号：
    CSB-PA885689LA01HU
  • 规格：
    ￥440
  • 促销：
    小规格抗体限时一口价
  • 图片：
    • Western Blot
      Positive WB detected in: Hela whole cell lysate, U87 whole cell lysate, U251 whole cell lysate, MCF-7 whole cell lysate, Rat heart tissue
      All lanes: ANO2 antibody at 3.9µg/ml
      Secondary
      Goat polyclonal to rabbit IgG at 1/50000 dilution
      Predicted band size: 114 kDa
      Observed band size: 114 kDa
    • IHC image of CSB-PA885689LA01HU diluted at 1:600 and staining in paraffin-embedded human small intestine tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
    • Immunofluorescence staining of HepG2 cells with CSB-PA885689LA01HU at 1:200, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
  • 其他：

产品详情

  • 产品名称：
    Rabbit anti-Homo sapiens (Human) ANO2 Polyclonal antibody
  • Uniprot No.：
    Q9NQ90
  • 基因名：
    ANO2
  • 别名：
    Ano2 antibody; ANO2_HUMAN antibody; Anoctamin 2 antibody; Anoctamin-2 antibody; C12orf3 antibody; Chromosome 12 open reading frame 3 antibody; DKFZp434P102 antibody; Transmembrane protein 16B (eight membrane-spanning domains) antibody; Transmembrane protein 16B antibody
  • 宿主：
    Rabbit
  • 反应种属：
    Human, Rat
  • 免疫原：
    Recombinant Human Anoctamin-2 protein (1-83AA)
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated

    本页面中的产品，ANO2 Antibody (CSB-PA885689LA01HU)，的标记方式是Non-conjugated。对于ANO2 Antibody，我们还提供其他标记。见下表：

    可提供标记
    标记方式 货号 产品名称 应用
    HRP CSB-PA885689LB01HU ANO2 Antibody, HRP conjugated ELISA
    FITC CSB-PA885689LC01HU ANO2 Antibody, FITC conjugated
    Biotin CSB-PA885689LD01HU ANO2 Antibody, Biotin conjugated ELISA
  • 克隆类型：
    Polyclonal
  • 抗体亚型：
    IgG
  • 纯化方式：
    >95%, Protein G purified
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, WB, IHC, IF
  • 推荐稀释比：
    Application Recommended Dilution
    WB 1:500-1:5000
    IHC 1:500-1:1000
    IF 1:200-1:500
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Calcium-activated chloride channel (CaCC) which may play a role in olfactory signal transduction. Odorant molecules bind to odor-sensing receptors (OSRs), leading to an increase in calcium entry that activates CaCC current which amplifies the depolarization of the OSR cells, ANO2 seems to be the underlying chloride channel involved in this process. May mediate light perception amplification in retina.
  • 基因功能参考文献：
    1. residues facing the putative channel pore are responsible both for controlling the ion selectivity and the gating of the channel, providing an initial understanding of molecular mechanism of ion permeation in TMEM16B PMID: 28046119
    2. data revealed prominently increased autoantibody reactivity against the chloride-channel protein anoctamin 2 (ANO2) in multiple sclerosis cases compared with controls PMID: 26862169
    3. Olfactory function impairment in Italian patients with type 3 von Willebrand disease with partial deletion of TMEM16B. PMID: 25635880
    4. ANO2 is present in human myometrium. PMID: 24928056
    5. The third intracellular loop of TMEM16B is the site involved in calcium ion sensitivity, whereas the C-terminal part affects the rate of transition between the open and the closed state of the channel. PMID: 23570556
    6. VWF and TMEM16B deletions may have a role in severe von Willebrand disease type 3 PMID: 17371490
    7. This study suggested that TMEM16B to be a strong candidate for the long sought-after Ca(2+)-dependent chloride channel in the photoreceptor synapse. PMID: 19474308
    8. Ano2 likely amplifies the odor-induced generator potential in olfactory sensory neuron cilia by sensing elevated calcium levels, permitting the outward flow of chloride ions from the cell. PMID: 19561302
    9. C12orf3, FLJ10261 (ORAOV2), C11orf25 and FLJ34272 constitute a family of eight-transmembrane proteins with N- and C-terminal tails facing the cytoplasm. PMID: 12739008

    显示更多

    收起更多

  • 亚细胞定位：
    Cell membrane; Multi-pass membrane protein.
  • 蛋白家族：
    Anoctamin family
  • 组织特异性：
    Retina, especially in the photoreceptor synaptic terminals.
  • 数据库链接：

    HGNC: 1183

    OMIM: 610109

    KEGG: hsa:57101

    STRING: 9606.ENSP00000314048

    UniGene: Hs.148970