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  4. MRC1 Antibody

MRC1 Antibody

  • 中文名称：
    MRC1兔多克隆抗体
  • 货号：
    CSB-PA338680XA01SVG
  • 规格：
    ￥440
  • 促销：
    小规格抗体限时一口价
  • 图片：
    • Western Blot
      Positive WB detected in: recombinant protein
      All lanes: MRC1 Antibody at 1:1000
      Secondary
      Goat polyclonal to rabbit IgG at 1/50000 dilution
      Predicted band size: 51 kDa
      Observed band size: 51 kDa
  • 其他：

产品详情

  • Uniprot No.：
    P25588
  • 基因名：
  • 别名：
    Mediator of replication checkpoint protein 1 (DNA replication checkpoint mediator MRC1) MRC1 YCL061C YCL61C/YCL60C
  • 反应种属：
    Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
  • 免疫原：
    Recombinant Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) MRC1 protein (711-851aa)
  • 免疫原种属：
    Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
  • 标记方式：
    Non-conjugated
  • 克隆类型：
    Polyclonal
  • 抗体亚型：
    IgG
  • 纯化方式：
    Affinity-chromatography
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, WB
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Required for normal DNA replication. Phosphorylated in response to DNA replication stress. Phosphorylation allows it to mediate the activation of RAD53.
  • 基因功能参考文献：
    1. An unbiased kinome screening identified several kinases that phosphorylate Mrc1 at the N terminus upon different environmental stresses. PMID: 29371596
    2. Dia2-dependent degradation of Mrc1 is required for replication fork restart in a pathway that includes Sgs1 and Mph1. PMID: 27956499
    3. Disruption of MRC1 leads to consistently elevated expansion and contraction rates of CAG.CTG repeat tracts. PMID: 18321795
    4. s show that minisatellite length expansions are controlled by the products of the CSM3 and TOF1 genes, while contractions are controlled by MRC1. PMID: 23165348
    5. Whereas Ddc1 and Dpb11 aid in replication-checkpoint activation, colocalization of Mec1 and Mrc1 at stalled replication forks promotes Rad53 activation sufficient to stabilize the replisome during transient replication stress. PMID: 22298423
    6. Data show that that ISC1 controls cell morphology in combination with checkpoint regulators Swe1, Cdk1(CDC28), MRC1, TOF1, CSM3, RAD9 and Rad53. PMID: 21840863
    7. cells deleted of MRC1, a gene implicated in replication fork stabilization and in the replication checkpoint pathway, maintained wild-type firing times despite over twofold lengthening of S phase PMID: 20219942
    8. Data show that the Tof1/Csm3/Mrc1 checkpoint complex interacts directly with the MCM helicase during both replication fork progression and when the replication fork is stalled. PMID: 15755447
    9. Mrc1 functions constitutively to promote normal replication fork progression. PMID: 16137624
    10. Tof1p-Csm3p function differently from MRC1 and their complex counteracts the Rrm3p helicase to control replication termination of Saccharomyces cerevisiae PMID: 16418273
    11. Mrc1 plays a specific role in DNA replication, promoting the Srs2 recruitment to proliferating cell nuclear antigen (PCNA) independently of checkpoint signaling. PMID: 16724109
    12. Mrc1 may negatively regulate Cdc45 and MCM helicase to render stalled forks capable of resuming replication. PMID: 16849602
    13. Study reports that in the absence of Mrc1, a component of the replication forks, telomeres of cdc13 or yku70 mutants exhibited increased degradation, while telomerase-negative cells displayed accelerated senescence. PMID: 17323081
    14. analyzed sister-chromatid cohesion in double mutants of mrc1Delta, tof1Delta, and csm3Delta and identified additive cohesion defects that indicated the existence of at least two pathways that contribute to sister-chromatid cohesion PMID: 17483413
    15. Mrc1 and Tof1 control the normal progression of DNA replication forks in distinct ways. PMID: 17652453
    16. In unperturbed cells, Mrc1 interacts independently with both the N-terminal and C-terminal halves of Pol2 (Pol2N and Pol2C). PMID: 18851837
    17. Data show that the replication checkpoint suppresses the formation of Rad52 foci in an Mrc1-dependent manner and prevents homologous recombination (HR) at chromosome breaks induced by the HO endonuclease. PMID: 19322196
    18. Rad53 is activated by Mec1 through adaptor protein Mrc1 PMID: 19457865
    19. Mec1-dependent, Rad53-independent phosphorylation of Mrc1 is required to establish a positive feedback loop that stabilizes Mec1 and the replisome at stalled forks. PMID: 19515819
    20. An Mcm helicase acts as a checkpoint sensor for methyl methanesulfonate-induced DNA damage through direct binding to the replication checkpoint mediator Mrc1. PMID: 19620285
    21. Csm3, Tof1, and Mrc1 form a heterotrimeric mediator complex that associates with DNA replication forks PMID: 19819872

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  • 亚细胞定位：
    Nucleus. Note=Associated with chromatin during S phase.
  • 数据库链接：

    KEGG: sce:YCL061C

    STRING: 4932.YCL061C