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SPT16 Antibody

  • 中文名称：
    SPT16兔多克隆抗体
  • 货号：
    CSB-PA022955XA01SVG
  • 规格：
    ￥440
  • 促销：
    小规格抗体限时一口价
  • 图片：
    • Western Blot
      Positive WB detected in: recombinant protein
      All lanes: SPT16 Antibody at 1:1000
      Secondary
      Goat polyclonal to rabbit IgG at 1/50000 dilution
      Predicted band size: 65 kDa
      Observed band size: 65 kDa
  • 其他：

产品详情

  • Uniprot No.：
    P32558
  • 基因名：
    SPT16
  • 别名：
    FACT complex subunit SPT16 (Cell division control protein 68) (Facilitates chromatin transcription complex subunit SPT16) (Suppressor of Ty protein 16) SPT16 CDC68 SSF1 YGL207W
  • 反应种属：
    Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
  • 免疫原：
    Recombinant Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SPT16 protein (647-964aa)
  • 免疫原种属：
    Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
  • 标记方式：
    Non-conjugated
  • 克隆类型：
    Polyclonal
  • 抗体亚型：
    IgG
  • 纯化方式：
    Affinity-chromatography
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, WB
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Component of the FACT complex, a general chromatin factor that acts to reorganize nucleosomes. The FACT complex is involved in multiple processes that require DNA as a template such as mRNA elongation, DNA replication and DNA repair. During transcription elongation the FACT complex acts as a histone chaperone that both destabilizes and restores nucleosomal structure. It facilitates the passage of RNA polymerase II and transcription by promoting the dissociation of one histone H2A-H2B dimer from the nucleosome, then subsequently promotes the reestablishment of the nucleosome following the passage of RNA polymerase II. Transcription elongation is promoted by the repression of transcription initiation from cryptic sites. Also acts in establishing transcription initiation complexes and promotes SPT15/TBP-binding to a TATA box. Together with replication factor-A protein (RPA), FACT may play a role in nucleosome deposition during DNA replication.
  • 基因功能参考文献：
    1. SPT16 interactions with nucleosome. PMID: 29271283
    2. Results show that Spt16 depletion results in global changes in nucleosome fuzziness and position shifts and that the majority of these changes are suppressed by inhibiting RNA polymerase II supporting a model in which Spt16 has a role in maintaining local nucleosome stability. PMID: 27141053
    3. These results suggest that the large scale overlap in Mot1 and Spt16 function arises from a combination of both their unique and shared functions in transcription complex assembly and chromatin structure regulation. PMID: 27226635
    4. Spt6 and FACT have both overlapping and distinct functional interactions with histones and nucleosomes and that binding to each of the components of a nucleosome is not sufficient to produce nucleosome reorganization PMID: 26416482
    5. results suggest that the discrimination of Spt16 to Htz1-bound genes is due to the priority of Chz1 over Spt16 in binding to the Htz1-bound genomic regions; Chz1-escorted Htz1 therefore impairs Spt16 binding at chromatin PMID: 24707933
    6. The Spt16 subunit of FACT is specifically required for H2B deposition onto rDNA genes. PMID: 23689130
    7. Structure of the Spt16 middle domain reveals functional features of the histone chaperone FACT. PMID: 23417676
    8. Mutation that encodes the Spt16 subunit of FACT causes phenotypes associated with defects in transcription and replication. PMID: 21625001
    9. These findings identify Spt16 as an Rtt101 substrate, and suggest that Spt16 ubiquitylation is important for FACT to function during DNA replication. PMID: 20634314
    10. The FACT subunit Spt16 and Pkc1 signaling have an overlapping essential function, with an unexpected role for FACT in the maintenance of cell integrity. PMID: 19727824
    11. N-terminal region of Polalpha (Pol1p) that is evolutionarily conserved among different species interacts with Spt16p-Pob3p and Ctf4p in vivo PMID: 15485923
    12. The Spt16 N-terminal domain does become important under conditions of replication stress, where it mediates adaptation to stress conditions. PMID: 15520471
    13. Chd1 and SPT16 and POB3 act in opposition in regulating transcription. PMID: 17620414
    14. neither the Spt16 NTD nor a key residue in the putative Pob3-M-binding site was required for interactions with histone N termini or for yFACT-mediated nucleosome reorganization PMID: 18089575
    15. Spt16 restores normal nucleosome structure by redepositing the displaced H3-H4 histones, thereby preventing incorporation of new histones. PMID: 19683500

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  • 亚细胞定位：
    Nucleus. Chromosome. Note=Colocalizes with RNA polymerase II on chromatin. Recruited to actively transcribed loci.
  • 蛋白家族：
    Peptidase M24 family, SPT16 subfamily
  • 数据库链接：

    KEGG: sce:YGL207W

    STRING: 4932.YGL207W