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  4. Recombinant Enterobacteria phage T5 Exodeoxyribonuclease (D15)

Recombinant Enterobacteria phage T5 Exodeoxyribonuclease (D15)

  • 中文名称：
    Recombinant Enterobacteria phage T5 Exodeoxyribonuclease(D15),Yeast
  • 货号：
    CSB-YP356750ELS
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    Recombinant Enterobacteria phage T5 Exodeoxyribonuclease(D15),Yeast
  • 货号：
    CSB-EP356750ELS
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    Recombinant Enterobacteria phage T5 Exodeoxyribonuclease(D15),Yeast
  • 货号：
    CSB-EP356750ELS-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    Recombinant Enterobacteria phage T5 Exodeoxyribonuclease(D15),Yeast
  • 货号：
    CSB-BP356750ELS
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    Recombinant Enterobacteria phage T5 Exodeoxyribonuclease(D15),Yeast
  • 货号：
    CSB-MP356750ELS
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    D15
  • Uniprot No.：
  • 别名：
    D15; exo5; T5.130; T5p128Flap endonuclease; FEN; EC 3.1.11.-; 5'-3' exonuclease; Exodeoxyribonuclease; EC 3.1.11.3
  • 种属：
    Enterobacteria phage T5 (Bacteriophage T5)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    2-291
  • 氨基酸序列
    SKSWGKFIE EEEAEMASRR NLMIVDGTNL GFRFKHNNSK KPFASSYVST IQSLAKSYSA RTTIVLGDKG KSVFRLEHLP EYKGNRDEKY AQRTEEEKAL DEQFFEYLKD AFELCKTTFP TFTIRGVEAD DMAAYIVKLI GHLYDHVWLI STDGDWDTLL TDKVSRFSFT TRREYHLRDM YEHHNVDDVE QFISLKAIMG DLGDNIRGVE GIGAKRGYNI IREFGNVLDI IDQLPLPGKQ KYIQNLNASE ELLFRNLILV DLPTYCVDAI AAVGQDVLDK FTKDILEIAE Q
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Catalyzes both the 5'-exonucleolytic and structure-specific endonucleolytic hydrolysis of DNA branched nucleic acid molecules and probably plays a role in viral genome replication. Active on flap (branched duplex DNA containing a free single-stranded 5'-end), 5'overhangs and pseudo-Y structures. The substrates require a free, single-stranded 5' end, with endonucleolytic hydrolysis occurring at the junction of double- and single-stranded DNA. This function may be used for example to trim such branched molecules generated by Okazaki fragments synthesis during replication.
  • 基因功能参考文献：
    1. Direct observation of DNA threading in flap endonuclease complexes with DNA has been reported. PMID: 27273516
  • 数据库链接：

    KEGG: vg:2777611