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  4. Recombinant Human 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 4 (PFKFB4)

Recombinant Human 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 4 (PFKFB4)

  • 中文名称：
    人PFKFB4重组蛋白
  • 货号：
    CSB-YP613704HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    人PFKFB4重组蛋白
  • 货号：
    CSB-EP613704HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    人PFKFB4重组蛋白
  • 货号：
    CSB-EP613704HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    人PFKFB4重组蛋白
  • 货号：
    CSB-BP613704HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    人PFKFB4重组蛋白
  • 货号：
    CSB-MP613704HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    6-bisphosphatase; 6-P2ase 4; 6-P2ASE testis-type isozyme; 6-phosphofructo-2-kinase; 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 4; 6PF 2 K/Fru 2,6 P2ase 4; 6PF 2 K/Fru 2,6 P2ase testis type isozyme; 6PF-2-K/Fru-2; 6PF-2-K/Fru-2,6-P2ase ; Bifunctional enzyme with kinase and biphosphatase activities; F264_HUMAN; Fructose-2; Fructose-2,6-bisphosphatase; PFK/FBPase 4; PFKFB4
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-469
  • 氨基酸序列
    MASPRELTQN PLKKIWMPYS NGRPALHACQ RGVCMTNCPT LIVMVGLPAR GKTYISKKLT RYLNWIGVPT REFNVGQYRR DVVKTYKSFE FFLPDNEEGL KIRKQCALAA LRDVRRFLSE EGGHVAVFDA TNTTRERRAT IFNFGEQNGY KTFFVESICV DPEVIAANIV QVKLGSPDYV NRDSDEATED FMRRIECYEN SYESLDEDLD RDLSYIKIMD VGQSYVVNRV ADHIQSRIVY YLMNIHVTPR SIYLCRHGES ELNLKGRIGG DPGLSPRGRE FAKSLAQFIS DQNIKDLKVW TSQMKRTIQT AEALGVPYEQ WKVLNEIDAG VCEEMTYEEI QDNYPLEFAL RDQDKYRYRY PKGESYEDLV QRLEPVIMEL ERQENVLVIC HQAVMRCLLA YFLDKAAEQL PYLKCPLHTV LKLTPVAYGC KVESIFLNVA AVNTHRDRPQ NVDISRPPEE ALVTVPAHQ
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Synthesis and degradation of fructose 2,6-bisphosphate.
  • 基因功能参考文献：
    1. PFKFB4 enhances the invasiveness of breast cancer cells in vitro and in vivo by inducing HA production and PFKFB4-induced expression of HAS2 depends upon the activation of p38 signaling. PMID: 30415245
    2. findings suggest that the Warburg pathway enzyme PFKFB4 acts as a molecular fulcrum that couples sugar metabolism to transcriptional activation by stimulating SRC-3 to promote aggressive metastatic tumours PMID: 29615789
    3. Data show that PFKFB4 is the target gene of PPARgamma which modulates the transcriptional activity of its promoter. PMID: 27769068
    4. Our FD models could facilitate a better mechanistic understanding of FD and help develop effective therapeutics for FD and other fibrosis diseases. PMID: 27614159
    5. High PFKFB4 expression is associated with colon and lung cancer. PMID: 28092678
    6. HIF-1alpha transactivates hypoxia-responsive elements (HRE)-D of the promoter region of PFKFB4 in hypoxia condition. PMID: 27181362
    7. Inhibition of PFKFB4 suppresses glycolysis and proliferation of multiple human cancer cell lines. PMID: 26221874
    8. PFKFB4 suppresses oxidative stress and p62 accumulation, without which autophagy is stimulated likely as a ROS detoxification response. PMID: 25772235
    9. Data indicate that the PFKFB4 expressed in multiple transformed cells and tumors functions to synthesize F2,6BP. PMID: 25115398
    10. we found that the glycolytic enzyme PFKFB4 is essential for prostate cancer cell survival by maintaining the balance between the use of glucose for energy generation and the synthesis of antioxidants. PMID: 22576210
    11. PFKFB4 has an important role in the progression of non-muscle-invasive bladder cancer ( PMID: 21396842
    12. PFKFB4 and HO-2 are expressed in a coordinated manner to maintain glucose homeostasis. PMID: 22892400
    13. Data suggest that molecular mechanism by which dihydrotestosterone induces Pfkfb4 (and thus glycolysis) during spermatogenesis involves stimulation of Sertoli cells to secrete FGF-2 (fibroblast growth factor 2); study uses recombinant human Pfkfb4. PMID: 22811469
    14. an essential role of PFKFB4 in the maintenance of brain cancer stem-like cells PMID: 22056879
    15. Sulforaphane is a potent inducer of apoptosis in hepatocellular carcinoma cells via PFKFB4-inhibition pathways. PMID: 21640852
    16. Mechanisms of hypoxic regulation of PFKFB4 gene expression were studied in several cancer cell lines. PMID: 15474002
    17. A detailed analysis of the 5'-flanking region of human gene pfkfb4 using different 5'-deletion promoter constructs was carried out; this gene is activated by serum and chemical hypoxia whereas beta-estradiol decreases its expression. PMID: 15642344
    18. Overexpression of PFKFB-4 transcript levels in breast & colon malignant tumors correlates with enhanced expression of PFKFB-3, hypoxia-inducible factor(HIF)-1alpha & known HIF-1 dependent genes Glut1 & VEGF PMID: 15925437

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  • 蛋白家族：
    Phosphoglycerate mutase family
  • 数据库链接：

    HGNC: 8875

    OMIM: 605320

    KEGG: hsa:5210

    STRING: 9606.ENSP00000232375

    UniGene: Hs.476217