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  4. Recombinant Human Z-DNA-binding protein 1 (ZBP1)

Recombinant Human Z-DNA-binding protein 1 (ZBP1)

  • 中文名称：
    人ZBP1重组蛋白
  • 货号：
    CSB-YP861990HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    人ZBP1重组蛋白
  • 货号：
    CSB-BP861990HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    人ZBP1重组蛋白
  • 货号：
    CSB-MP861990HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    C20orf183; DAI; DLM 1; DLM1; DNA-dependent activator of interferon regulatory factors; DNA-dependent activator of IRFs; OTTHUMP00000031373; OTTHUMP00000174290; OTTHUMP00000237854; Tumor stroma and activated macrophage protein DLM 1; Tumor stroma and activated macrophage protein DLM-1; Z-DNA-binding protein 1; ZBP 1; Zbp1; ZBP1_HUMAN; ZDBP1
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-429
  • 氨基酸序列
    MAQAPADPGR EGHLEQRILQ VLTEAGSPVK LAQLVKECQA PKRELNQVLY RMKKELKVSL TSPATWCLGG TDPEGEGPAE LALSSPAERP QQHAATIPET PGPQFSQQRE EDIYRFLKDN GPQRALVIAQ ALGMRTAKDV NRDLYRMKSR HLLDMDEQSK AWTIYRPEDS GRRAKSASII YQHNPINMIC QNGPNSWISI ANSEAIQIGH GNIITRQTVS REDGSAGPRH LPSMAPGDSS TWGTLVDPWG PQDIHMEQSI LRRVQLGHSN EMRLHGVPSE GPAHIPPGSP PVSATAAGPE ASFEARIPSP GTHPEGEAAQ RIHMKSCFLE DATIGNSNKM SISPGVAGPG GVAGSGEGEP GEDAGRRPAD TQSRSHFPRD IGQPITPSHS KLTPKLETMT LGNRSHKAAE GSHYVDEASH EGSWWGGGI
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Key innate sensor that recognizes and binds Z-RNA structures, which are produced by a number of viruses, such as herpesvirus, orthomyxovirus or flavivirus, and triggers different forms of cell death. Once activated upon Z-RNA-binding, ZBP1 interacts with RIPK3, inducing the complementary pathways of apoptosis, necroptosis and pyroptosis. Acts as a key activator of necroptosis, a programmed cell death process in response to death-inducing TNF-alpha family members: ZBP1-dependent necroptosis involves RIPK3 stimulation, which phosphorylates and activates MLKL, triggering execution of programmed necrosis. In addition to TNF-induced necroptosis, necroptosis can also take place in the nucleus in response to orthomyxoviruses infection: ZBP1 recognizes and binds Z-RNA structures that are produced in infected nuclei by orthomyxoviruses, such as the influenza A virus (IAV), leading to ZBP1 activation, RIPK3 stimulation and subsequent MLKL phosphorylation, triggering disruption of the nuclear envelope and leakage of cellular DNA into the cytosol. ZBP1-dependent cell death in response to IAV infection promotes interleukin-1 alpha (IL1A) induction in an NLRP3-inflammasome-independent manner: IL1A expression is required for the optimal interleukin-1 beta (IL1B) production, and together, these cytokines promote infiltration of inflammatory neutrophils to the lung, leading to the formation of neutrophil extracellular traps. In some cell types, also able to restrict viral replication by promoting cell death-independent responses. In response to Zika virus infection in neurons, promotes a cell death-independent pathway that restricts viral replication: together with RIPK3, promotes a death-independent transcriptional program that modifies the cellular metabolism via up-regulation expression of the enzyme ACOD1/IRG1 and production of the metabolite itaconate. Itaconate inhibits the activity of succinate dehydrogenase, generating a metabolic state in neurons that suppresses replication of viral genomes.; (Microbial infection) In case of herpes simplex virus 1/HHV-1 infection, forms hetero-amyloid structures with HHV-1 protein RIR1/ICP6 which may inhibit ZBP1-mediated necroptosis, thereby preventing host cell death pathway and allowing viral evasion.
  • 基因功能参考文献：
    1. this paper reviews the history and emergence of ZBP1 as a pathogen sensor and a central regulator of cell death and inflammatory responses PMID: 29236673
    2. PUMA promotes the cytosolic release of mitochondrial DNA and activation of the DNA sensors DAI/Zbp1 and STING, leading to enhanced RIP3 and MLKL phosphorylation in a positive feedback loop. PMID: 29581256
    3. There is a correlation between the decrease in DAI-1 receptor expression and the severity of disease progression in preeclampsia. PMID: 29127557
    4. Our results show that HSV2 is detected by a plethora of PRRs including DAI protein which trigger cytokine secretion to protect the host. PMID: 24080302
    5. DAI could function as a DNA sensor and a regulator in DNA-induced macrophage M2b polarization and lupus nephritis. PMID: 23553627
    6. Collectively, these results demonstrate that DAI can suppress HSV-1 growth independent of DNA sensing through mechanisms involving suppression of viral genomes and regulation of ICP0. PMID: 23283962
    7. The data of this study suggest that RNA-binding proteins can be used as a tool to identify the post-transcriptional regulation of gene expression in the establishment and function of neural circuits involved in addiction behaviors. PMID: 22240322
    8. these data indicate that ZBP1 may function as an adapter to export the Ro/Y3 RNA complex from nuclei. PMID: 22114317
    9. Solution structure of the Zbeta domain of human DNA-dependent activator of IFN-regulatory factors and its binding modes to B- and Z-DNAs. PMID: 21471454
    10. hZbeta(DAI) binds to Z-DNA via an active-di B-Z transition mechanism. PMID: 21296080
    11. These results suggest that activation of DAI might contribute to augment HIV-1 replication through DAI- NF-kappaB pathway. PMID: 20599623
    12. identify ZBP1 as being essential for IRF3 activation and interferon beta expression triggered by HCMV, as well as being sufficient to enhance HCMV-stimulated beta interferon transcription and secretion. PMID: 19846511
    13. molecular cloning and structure analysis PMID: 11842111
    14. Z-DNA binding activities of two Zalpha domains in the human ZBP1, hZalpha(ZBP1) and hZbeta(ZBP1)were characterized. PMID: 16448869
    15. In conclusion, intracellular bacteria and cytosolic poly(dA-dT) activate IFNbeta responses in different human cells without requiring human ZBP1. PMID: 18771559
    16. DAI binds to and colocalizes with endogenous adaptor receptor-interacting protein kinase (RIP)1 at characteristic cytoplasmic granules. PMID: 18941233
    17. binding 2 DAIs to 1 dsDNA brings about dimerization of DAI that might facilitate DNA-mediated innate immune activation. PMID: 19095800
    18. These data suggest that repression of ZBP1 by blocking beta-catenin binding at the ZBP1 promoter deregulates its associated mRNAs, leading to the phenotypic changes of breast cancers. PMID: 19461076

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  • 亚细胞定位：
    Cytoplasm. Nucleus.; [Isoform 7]: Cytoplasm. Nucleus.
  • 组织特异性：
    Highly expressed in lymphatic tissues including lymph node, leukocytes, tonsil, bone marrow and spleen. Expressed to a lesser extent in thymus, lung and liver.
  • 数据库链接：

    HGNC: 16176

    OMIM: 606750

    KEGG: hsa:81030

    STRING: 9606.ENSP00000360215

    UniGene: Hs.302123