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  4. Recombinant Rat CCAAT/enhancer-binding protein alpha (Cebpa)

Recombinant Rat CCAAT/enhancer-binding protein alpha (Cebpa)

  • 中文名称：
    Recombinant Rat CCAAT/enhancer-binding protein alpha(Cebpa),Yeast
  • 货号：
    CSB-YP005180RA
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    Recombinant Rat CCAAT/enhancer-binding protein alpha(Cebpa),Yeast
  • 货号：
    CSB-EP005180RA
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    Recombinant Rat CCAAT/enhancer-binding protein alpha(Cebpa),Yeast
  • 货号：
    CSB-EP005180RA-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    Recombinant Rat CCAAT/enhancer-binding protein alpha(Cebpa),Yeast
  • 货号：
    CSB-BP005180RA
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    Recombinant Rat CCAAT/enhancer-binding protein alpha(Cebpa),Yeast
  • 货号：
    CSB-MP005180RA
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Cebpa
  • Uniprot No.：
  • 别名：
    CebpaCCAAT/enhancer-binding protein alpha; C/EBP alpha
  • 种属：
    Rattus norvegicus (Rat)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-358
  • 氨基酸序列
    MESADFYEAE PRPPMSSHLQ SPPHAPSNAA FGFPRGAGPA PPPAPPAAPE PLGGICEHET SIDISAYIDP AAFNDEFLAD LFQHSRQQEK AKAAAGPAGG GGDFDYPGAP AGPGGAVMSA GAHGPPPGYG CAAAGYLDGR LEPLYERVGA PALRPLVIKQ EPREEDEAKQ LALAGLFPYQ PPPPPPPPHP HASPAHLAAP HLQFQIAHCG QTTMHLQPGH PTPPPTPVPS PHPAPAMGAA GLPGPGGSLK GLAGPHPDLR TGGGGGGGAG AGKAKKSVDK NSNEYRVRRE RNNIAVRKSR DKAKQRNVET QQKVLELTSD NDRLRKRVEQ LSRELDTLRG IFRQLPESSL VKAMGNCA
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Transcription factor that coordinates proliferation arrest and the differentiation of myeloid progenitors, adipocytes, hepatocytes, and cells of the lung and the placenta. Binds directly to the consensus DNA sequence 5'-TCan act as dominant-negative. Binds DNA and have transctivation activity, even if much less efficiently than isoform 2. Does not inhibit cell proliferation.; Directly and specifically enhances ribosomal DNA transcription interacting with RNA polymerase I-specific cofactors and inducing histone acetylation.
  • 基因功能参考文献：
    1. C/EBPalpha played a role in lung development and provided the insight into the mechanism underlying SUMO-modification. PMID: 28902364
    2. The differential regulation of hepcidin between young and old rats after hyperthermia may be due to the enhanced expression of C/EBPalpha protein in old rats. PMID: 23993269
    3. IL-13-enhanced PLA2 activity regulates COX-2/PGE2 expression through C/EBP-alpha activation. PMID: 23881867
    4. HDAC3 interacts with sumoylated CEBPalpha to negatively regulate the LXRalpha expression. PMID: 23639777
    5. This study indicated, for the first time, that diabetes mellitus altered the immunoreactivity of C/EBPs in the nervous system. PMID: 23508841
    6. the basic region and leucine zipper (BZip) domain of C/EBPalpha is sufficient for the interaction with HP1alpha in regions of heterochromatin PMID: 23392382
    7. Angelica sinensis polysaccharide downregulated hepcidin by repressing CCAAT/enhancer-binding protein alpha and the phosphorylation of signal transducer and activator of transcription 3/5. PMID: 22985399
    8. In rats subjected to intermittent fasting, PPARgamma2 and C/EBPalpha mRNA expression in white adipose tissue was increased, and there was a positive correlation of Fsp27 gene expression with PPARgamma2 and C/EBPalpha mRNA levels. PMID: 21996045
    9. in pleural mesothelioma, p38 delta and C/EBP-alpha mediate proliferation and thus may represent new targets in mesothelioma therapy PMID: 21227534
    10. iNOS promoter was the direct transcriptional target of the C/EBPalpha, C/EBPbeta, and NF-kappaB binding proteins in hepatocytes PMID: 21539866
    11. C/EBPalpha down-regulation may be one mechanism of reduced hepatocyte viability in cultured hypoxic rat primary hepatocytes PMID: 20219337
    12. Protein level of C/EBPalpha in lung of the STZ-diabetic rats was similar as the normal rats. PMID: 21249617
    13. C/EBPalpha, in cooperation with NFAT, directly regulates Il4 gene transcription. PMID: 21454593
    14. role in regulating hepatic transcription of hepcidin PMID: 12183449
    15. Transiently expressed C/EBPalpha induced PRL gene transcription in pituitary GHFT1-5 cells, whereas the coexpression of Pit-1 and C/EBPalpha in HeLa cells demonstrated their cooperativity at the PRL promoter PMID: 12554749
    16. Results of x-ray structure analysis of a C/EBPalpha bZIP polypeptide bound to its cognate DNA site and engineered amino acid substitutions provide the first detailed description of DNA recognition by a C/EBP family member. PMID: 12578822
    17. CEBPa has a role in regulating lipogenic enzymes and transport proteins stimulated by keratinocyte growth factor in type II alveolar cells PMID: 12865412
    18. Results describe the temporal relationships and role of CCAAT/enhancing-binding protein alpha, sterol regulatory element binding protein-1c, and stearoyl-CoA desaturase 1 in lipogenesis in fetal rat lung PMID: 12896875
    19. the anti-proliferation activity of C/EBPalpha critically depends on components of the SWI/SNF core complex PMID: 14660596
    20. C-EBP alpha has a role in the activation phenotype of hepatic stellate cells. PMID: 15504357
    21. Results show that in addition to its previously recognized function in suppressing PPARgamma transcriptional activity, interaction of GATA-2 and -3 with C/EBP is necessary for their ability to negatively regulate adipogenesis. PMID: 15632071
    22. Haptoglobin gene transcription is regulated by C/EBP alpha during normal liver development. PMID: 16172914
    23. A negative regulator of placental glutathione S-transferase gene expression in normal liver. PMID: 16407263
    24. the domain containing threonines 222/226 has a role in regulating C/EBPalpha activity in adipocytes but not hepatocytes, and GSK3 phosphorylation of these residues does not mediate regulation of this C/EBP activity PMID: 16600022
    25. In LNCaP cells C/EBPalpha over expression inhibits expression of prostate specific antigen by Androgen receptor-dependent mechanism PMID: 16774685
    26. HNF4alpha, CREM, HNF1alpha, and C/EBPalpha have roles in transcriptional regulation of the glucose-6-phosphatase gene by cAMP/vasoactive intestinal peptide in the intestine PMID: 16893891
    27. Data suggested that both the initiating and maintaining of differentiation process were regulated by PPARgamma, while C/EBPalpha was likely to participate in the maintaining of differentiation process only. PMID: 17966466
    28. Binds and activates the endogenous PU.1 (Spi-1) promoter, providing one mechanism for induction of monopoiesis. PMID: 18026136
    29. temporo-spatial expression pattern of the transcription factor C/EBPalpha, a key player in adipocyte differentiation PMID: 18328085
    30. The results indicated that the death receptor pathway was mainly involved and regulated by PPAR-gamma and p53 in the process of apoptosis triggered by C/EBP-alpha in hepatic stellate cells. PMID: 19168033

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  • 亚细胞定位：
    Nucleus.; [Isoform 4]: Nucleus, nucleolus.
  • 蛋白家族：
    BZIP family, C/EBP subfamily
  • 组织特异性：
    Isoform 2 and isoform 3 are expressed in liver (at protein level).
  • 数据库链接：

    KEGG: rno:24252

    STRING: 10116.ENSRNOP00000063123