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  4. Recombinant Rat Neuronal acetylcholine receptor subunit alpha-4 (Chrna4), partial

Recombinant Rat Neuronal acetylcholine receptor subunit alpha-4 (Chrna4), partial

  • 中文名称：
    Recombinant Rat Neuronal acetylcholine receptor subunit alpha-4(Chrna4),partial,Yeast
  • 货号：
    CSB-YP005390RA
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    Recombinant Rat Neuronal acetylcholine receptor subunit alpha-4(Chrna4),partial,Yeast
  • 货号：
    CSB-EP005390RA
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    Recombinant Rat Neuronal acetylcholine receptor subunit alpha-4(Chrna4),partial,Yeast
  • 货号：
    CSB-EP005390RA-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    Recombinant Rat Neuronal acetylcholine receptor subunit alpha-4(Chrna4),partial,Yeast
  • 货号：
    CSB-BP005390RA
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    Recombinant Rat Neuronal acetylcholine receptor subunit alpha-4(Chrna4),partial,Yeast
  • 货号：
    CSB-MP005390RA
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Chrna4
  • Uniprot No.：
  • 别名：
    Chrna4; Acra4; Neuronal acetylcholine receptor subunit alpha-4
  • 种属：
    Rattus norvegicus (Rat)
  • 蛋白长度：
    Partial
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane permeable to sodium ions.
  • 基因功能参考文献：
    1. the mechanism of interaction between [Upsilon4E]GID and the agonist binding pockets of the alpha4beta2 and the halpha7 receptors, and to estimate their relative binding affinities, was investigated. PMID: 27721068
    2. findings indicate that alterations in expression of the alpha4beta2 subtype of nAChR may be involved in the molecular mechanism(s) underlying the cognitive deficit associated with vascular dementia PMID: 26858154
    3. Data indicate that compounds showed favorable subtype selectivity for beta2*-nAChRs over beta4*-nAChRs. PMID: 23734673
    4. Artificial food colors and additives led to a decrease in the expressions of nAChR alpha4 subunits in male rats. PMID: 23429044
    5. Data suggest that alpha4 beta2 nicotinic receptors (Chrna4; Chrnb2) are involved in tolerance to DOPA exhibited as drug-induced dyskinesias in late stages of Parkinson's disease. PMID: 23583932
    6. The azabicyclic alcohol was found to compete with MTSEA between residues 6' & 13' in a state-dependent manner, but the reactive probe only bonded with 13' in the open state, suggesting that the 13' position is the dominant binding site. PMID: 22778816
    7. Data indicate that functional alpha4/beta2 nicotinic acetylcholine receptors (alpha4beta2 nAChR) plays a role in proliferation and differentiation fate into a neuronal lineage in neural progenitors of developing neocortex. PMID: 23056257
    8. nAChR alpha4 subtype contributes to the cholinergic nicotinic regulation of catecholamine signaling in the carotid body system. PMID: 22127290
    9. Our results identify a novel palmitoylation site on cysteine 273 in the M1-M2 loop of the alpha4 nAChR and determine that cysteines in both intracellular loops are regulatory factors in total and cell surface protein expression of the alpha4beta2 nAChR. PMID: 22593584
    10. The number of functional alpha4beta2 heteromeric neuronal nicotinic receptors is decreased in the brains of rats with severe renal failure. PMID: 22510484
    11. Nicotine-induced upregulation of the nAChR alpha4beta2 receptor demonstrates that at least two different kinetic mechanisms underlie multiple processes occurring at different rates in rat cortical neurons in real time. PMID: 22323734
    12. the lower alpha4beta2 nAChR number in spontaneously hypertensive rat compared with WKY may be a consequence of dysfunctional post-transcriptional regulation of nAChRs PMID: 21824140
    13. central alpha4beta2 nicotinic acetylcholine receptors that enable a provoking effect of endogenous acetylcholine play a critical role in impulsive action PMID: 20639140
    14. Data show that a hydrogen bond is functionally significant in the alpha4beta2 neuronal nAChR. PMID: 20616056
    15. Suggest a critical role for alpha4beta2 nAChRs in the infralimbic cortex in mediating the effects of nicotine on impulsive-like actions. PMID: 20238211
    16. Electrophysiological properties of Chrna4 indicate that Ser-284 plays an important role in the gating of nAChR along with Thr-276 and Ser-280 and suggest that the mutation at Ser-284 reduces nAChR activity similarly to other mutations of alpha 4 subunit. PMID: 11904236
    17. a role for alpha4beta2-like AChRs, but not alpha7 AChRs, in rapid excitatory transmission between alpha-motor neurons and Renshaw cells in rat spinal cord. PMID: 12037212
    18. surface expression levels and functional properties modulated by visinin-like protein-1 in response to changes in calcium levels PMID: 12202488
    19. Ca2+ modulation may prevent presynaptic alpha4beta2 nAChRs from overstimulating glutamate release at central excitatory synapses during bouts of synchronous, repetitive activity; could trigger seizures PMID: 12754307
    20. the alpha4 subunit of the nicotinic receptor is positioned to mediate the effects of acetylcholine widely across many, if not all, monoaminergic neurons in the brainstem PMID: 12774304
    21. At least two different affinity sites for acetylcholine. Sensitivity to block of alpha4beta2 channels by dihydro-beta-erythroidine was higher than that for alpha2beta2. Current-voltage for alpha2beta2 more strongly rectified than for alpha4beta2. PMID: 15026122
    22. Nicotinic acetylcholine receptor alpha 4 subunit is localized in virtually all nociceptive neurons that are identified by immunoreactivity for the vanilloid receptor TRPV-1. PMID: 15296793
    23. We propose that the enhanced expression of alpha4 nAChR by astrocytes stimulated directly by nanomolar concentrations of Abeta(1-42) might be related to ongoing defensive or compensative mechanisms. PMID: 15955596
    24. Mutation of F350, L351, L357, & L358 to alanine in the alpha4 AChR subunit attenuates cell surface expression of mutant alpha4beta2 AChRs. There may be a cooperative or regulatory role for the alpha4 AChR subunit in ER export. PMID: 16014729
    25. close spatial apposition of full-length P2X2 and alpha4beta2 channels within 100 nm of the plasma membrane of living cells PMID: 16033901
    26. analysis of single channel conductance within the large cytoplasmic loop of 5-hydroxytryptamine type 3 and alpha4beta2 nicotinic acetylcholine receptors PMID: 16407231
    27. alpha4beta2 nicotinic acetylcholine receptors may play a role in ethanol-seeking behaviors in animals chronically exposed to ethanol PMID: 17626178
    28. alpha-4 nicotinic acetylcholine receptors by GABAergic neurons of rostral ventral medulla and caudal pons. PMID: 17950703
    29. alpha4 containing receptors are positioned to influence 5-HT neurons directly at postsynaptic sites. PMID: 18403129
    30. data indicate that alpha6beta2(*) nAChR-evoked dopamine release in nicotine-treated rats is mediated by the alpha6(nonalpha4)beta2(*) nAChR subtype PMID: 18583454
    31. Results show that amyloid beta protein induces suppression of long-term potentiation in rat hippocampus, and that this suppression is mediated through alpha4beta2 nicotinic acetylcholine receptors. PMID: 18602971
    32. Phosphorylation at multiple consensus sites in the alpha4 subunit could play a remarkable role on the regulation of the functional expression of the alpha4beta2 nAChR. PMID: 18818999
    33. Nicotinic alpha4 beta2 receptors partly mediate brainstem autonomic dysfunction evoked by the inhaled anesthetic isoflurane. PMID: 19095841
    34. Neurexin-1beta targets AChR alpha4 and AChR beta2 to presynaptic terminals. PMID: 19567877

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  • 亚细胞定位：
    Cell junction, synapse, postsynaptic cell membrane; Multi-pass membrane protein. Cell membrane; Multi-pass membrane protein. Cell membrane; Lipid-anchor.
  • 蛋白家族：
    Ligand-gated ion channel (TC 1.A.9) family, Acetylcholine receptor (TC 1.A.9.1) subfamily, Alpha-4/CHRNA4 sub-subfamily
  • 组织特异性：
    In various regions of the central nervous system.
  • 数据库链接：

    KEGG: rno:25590

    STRING: 10116.ENSRNOP00000009041

    UniGene: Rn.9697