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Rat Low Density Lipoprotein cholesterol (LDL-C) ELISA kit

  • 中文名称：
    大鼠低密度脂蛋白胆固醇(LDL-C)酶联免疫试剂盒
  • 货号：
    CSB-E16561r
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Rat LDL-C ELISA Kit was designed for the quantitative measurement of Rat LDL-C protein in serum, plasma, tissue homogenates. It is a Competitive ELISA kit, its detection range is 125 ng/mL-8000 ng/mL and the sensitivity is 62.5 ng/mL.

  • 缩写：
    LDL-C
  • 种属：
    Rattus norvegicus (Rat)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    125 ng/mL-8000 ng/mL
  • 灵敏度：
    62.5 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Cardiovascular
  • 测定原理：
    quantitative
  • 测定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat LDL-C in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:100 Average % 98  
    Range % 94-102  
    1:200 Average % 94  
    Range % 91-97  
    1:400 Average % 102  
    Range % 98-106  
    1:800 Average % 88  
    Range % 84-92  
  • 回收率：
    The recovery of rat LDL-C spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 93 89-99  
    EDTA plasma (n=4) 103 98-109  
                 
                 
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average    
    8000 0.089 0.088 0.089    
    4000 0.182 0.178 0.180    
    2000 0.376 0.369 0.373    
    1000 0.630 0.642 0.636    
    500 1.130 1.146 1.138    
    250 1.528 1.516 1.522    
    125 1.914 1.810 1.862    
    0 2.267 2.169 2.218    
  • 数据处理：
  • 货期：
    3-5 working days

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