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Mouse amyloid beta peptide 1-42,Aβ1-42 ELISA Kit

  • 中文名称：
    小鼠β淀粉样蛋白1-42(Aβ1-42)酶联免疫试剂盒
  • 货号：
    CSB-E10787m
  • 规格：
    96T/48T
  • 价格：
    ￥3800/￥2500
  • 其他：

产品详情

  • 产品描述：
        Aβ1-42 (Amyloid beta 1-42)是由脑细胞中酶β-和γ-分泌酶所作用的Amyloid precursor protein (APP)切割而成的42个氨基酸长的肽段。Aβ1-42在早期阶段是神经退行性疾病如阿尔茨海默病的标志物之一。在病变形成的初期，Aβ1-42聚集成神经毒性的β淀粉样物，进而导致神经元死亡和神经退化。因此，Aβ1-42在诊断和研究神经退行性疾病方面具有重要意义。Aβ1-42的含量可以通过脑脊液和血液等生物标本检测。
        j9九游会登录入口首页生物所提供的Mouse amyloid beta peptide 1-42,Aβ1-42 ELISA Kit属于ELISA检测试剂盒，采用双抗夹心法定量检测鼠血清、血浆、组织匀浆样本中的Aβ1-42，其灵敏度为3.9 pg/mL，检测范围为15.6 pg/mL-1000 pg/mL。
     
  • 别名：
    Amyloid beta 42(ABeta 42),amyloid beta peptide 1-42,Aβ1-42,amyloid βpeptide 1-42
  • 缩写：
    Aβ1-42
  • 种属：
    Mus musculus (Mouse)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    15.6 pg/mL-1000 pg/mL
  • 灵敏度：
    3.9 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Neuroscience
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse Aβ1-42 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:1 Average % 92  
    Range % 88-99  
    1:2 Average % 95  
    Range % 85-105  
    1:4 Average % 96  
    Range % 90-105  
    1:8 Average % 93  
    Range % 86-100  
  • 回收率：
    The recovery of mouse Aβ1-42 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 95 90-100  
    EDTA plasma (n=4) 93 85-96  
                 
                 
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected  
    1000 2.490 2.451 2.471 2.337  
    500 2.049 1.985 2.017 1.883  
    250 1.357 1.385 1.371 1.237  
    125 0.866 0.837 0.852 0.718  
    62.5 0.517 0.524 0.521 0.387  
    31.2 0.347 0.355 0.351 0.217  
    15.6 0.244 0.228 0.236 0.102  
    0 0.135 0.132 0.134 　  
  • 数据处理：
  • 货期：
    3-5 working days

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问答及客户评论

■ 常见问题解答
Q:

Hi I would like to ask the following for the item Mouse amyloid beta peptide 1-40 ELISA kits:
1. How may test samples can be performed in 1 kit, is it possible to use the kit for 1 96-well plate

A:
Thanks for your inquiry. This is a 96T sandwich ELISA kit (double-antibody). For a 96T sandwich ELISA kit we suggest you run duplicates for the standard. In this way you can test 80 samples. We also suggest you run duplicates for the sample, and in this way you can test 40 samples. You can decide how many kits you need according to the number of your samples. If you run a pre-test, the number of samples you can detect in one kit will be less.

靶点详情

  • 最新研究进展：
    Aβ1-42是β淀粉样蛋白（Aβ）家族的一员，它是阿尔茨海默病（AD）病理学的主要成分。最近的研究表明，Aβ1-42的积聚是导致AD的主要因素之一。已经发现许多抗Aβ1-42药物，如免疫疗法和β-淀粉样蛋白药物，这些药物能够清除Aβ1-42，并恢复AD患者的认知能力。此外，一些研究还发现，Aβ1-42可能是癫痫的重要病理因素，这为癫痫治疗提供了新的思路。