Your Good Partner in Biology Research

  1. 首页
  2. 试剂盒
  3. 酶联免疫试剂盒
  4. Goat Interleukin 1β(IL-1β) ELISA Kit

Goat Interleukin 1β(IL-1β) ELISA Kit

  • 中文名称：
    山羊白介素1β(IL-1β)酶联免疫试剂盒
  • 货号：
    CSB-E16568G
  • 规格：
    96T/48T
  • 价格：
    ￥3900/￥2500
  • 其他：

产品详情

  • 产品描述：
    山羊白介素1β(IL-1β)酶联免疫试剂盒（CSB-E16568G）为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的IL1B含量。IL1B即白介素-1β，是重要炎症介质。它在炎症反应、免疫调节等生理病理过程中起关键作用。其研究机制主要围绕它与受体结合后激活下游信号通路，引发炎症级联反应，目前针对它的干预研究有望为炎症相关疾病治疗提供新思路。试剂盒检测范围为3.12 pg/mL-200 pg/mL，适用于动物模型研究、炎症机制探索、免疫调控实验等科研领域，能够为病原体感染评估、炎症因子网络分析、免疫干预效果验证等基础研究提供可靠数据支持本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
  • 别名：
    IL1BInterleukin-1 beta ELISA Kit; IL-1 beta ELISA Kit
  • 缩写：
    IL-1β
  • Uniprot No.：
  • 种属：
    Capra hircus (Goat)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    3.12 pg/mL-200 pg/mL
  • 灵敏度：
    0.78 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Immunology
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of goat IL-1β in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %87
    Range %82-94
    1:2Average %90
    Range %82-97
    1:4Average %92
    Range %85-100
    1:8Average %95
    Range %90-103
  • 回收率：
    The recovery of goat IL-1β spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9085-98
    EDTA plasma (n=4)9993-108
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    2002.112 2.052 2.082 1.951
    1001.576 1.654 1.615 1.484
    501.039 0.984 1.012 0.881
    250.587 0.549 0.568 0.437
    12.50.384 0.397 0.391 0.260
    6.250.292 0.269 0.281 0.150
    3.120.208 0.199 0.204 0.073
    00.127 0.134 0.131 　
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Potent proinflammatory cytokine. Initially discovered as the major endogenous pyrogen, induces prostaglandin synthesis, neutrophil influx and activation, T-cell activation and cytokine production, B-cell activation and antibody production, and fibroblast proliferation and collagen production. Promotes Th17 differentiation of T-cells. Synergizes with IL12/interleukin-12 to induce IFNG synthesis from T-helper 1 (Th1) cells. Plays a role in angiogenesis by inducing VEGF production synergistically with TNF and IL6.
  • 亚细胞定位：
    Cytoplasm, cytosol. Lysosome. Secreted, extracellular exosome. Secreted.
  • 蛋白家族：
    IL-1 family
  • 数据库链接：

    UniGene: Chi.15482



//