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  4. Mouse amyloid beta peptide 1-40,Aβ1-40 ELISA Kit

Mouse amyloid beta peptide 1-40,Aβ1-40 ELISA Kit

  • 中文名称：
    小鼠β淀粉样蛋白1-40(Aβ1-40)酶联免疫试剂盒
  • 货号：
    CSB-E08300m
  • 规格：
    96T/48T
  • 价格：
    ￥3800/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Mouse Aβ1-40 ELISA Kit was designed for the quantitative measurement of Mouse Aβ1-40 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 31.25 pg/mL-2000 pg/mL and the sensitivity is 7.8 pg/mL.

  • 别名：
    amyloid beta peptide 1-40,Aβ1-40,amyloid β peptide 1-40
  • 缩写：
    Aβ1-40
  • 种属：
    Mus musculus (Mouse)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    31.25 pg/mL-2000 pg/mL
  • 灵敏度：
    7.8 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Neuroscience
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse Aβ1-40 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:1Average %89
    Range %80-97
    1:2Average %95
    Range %90-105
    1:4Average %95
    Range %83-100
    1:8Average %93
    Range %83-98
  • 回收率：
    The recovery of mouse Aβ1-40 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9793-106
    EDTA plasma (n=4)8986-92
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    20002.637 2.739 2.688 2.512
    10002.115 2.092 2.104 1.928
    5001.318 1.287 1.303 1.127
    2500.824 0.810 0.817 0.641
    1250.491 0.504 0.498 0.322
    62.50.322 0.315 0.319 0.143
    31.250.265 0.273 0.269 0.093
    00.172 0.180 0.176
  • 数据处理：
  • 货期：
    3-5 working days

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问答及客户评论

■ 常见问题解答
Q:

Hi I would like to ask the following for the item Mouse amyloid beta peptide 1-40 ELISA kits:
1. How may test samples can be performed in 1 kit, is it possible to use the kit for 1 96-well plate

A:
Thanks for your inquiry. This is a sandwich ELISA kit (double-antibody). For a 96T sandwich ELISA kit, we suggest you run duplicates for the standard. In this way you can test 80 samples. We also suggest you run duplicates for the sample, and in this way you can test 40 samples. You can decide how many kits you need according to the number of your samples. If you run a pre-test, the number of samples you can detect in one kit will be less.


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