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  4. Rabbit Macrophage Inflammatory Protein-1α,MIP-1α ELISA kit

Rabbit Macrophage Inflammatory Protein-1α,MIP-1α ELISA kit

  • 中文名称：
    兔子巨噬细胞炎性蛋白1α(MIP-1α/CCL3)酶联免疫试剂盒
  • 货号：
    CSB-E04625Rb
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 别名：
    MIP-1 alpha,Macrophage Inflammatory Protein-1 alpha
  • 缩写：
    MIP1α/CCL3
  • 种属：
    Oryctolagus cuniculus (Rabbit)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    7.8 pg/mL-500 pg/mL
  • 灵敏度：
    1.95 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Immunology
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%        
    Three samples of known concentration were tested twenty times on one plate to assess.    
    Inter-assay Precision (Precision between assays): CV%<10%        
    Three samples of known concentration were tested in twenty assays to assess.      
                   
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rabbit MIP-1α in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
    　 Sample Serum(n=4)    
    1:1 Average % 105    
    Range % 93-109    
    1:2 Average % 110    
    Range % 97-114    
    1:4 Average % 88    
    Range % 83-93    
    1:8 Average % 95    
    Range % 85-98    
  • 回收率：
    The recovery of rabbit MIP-1α spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
     
    Sample Type Average % Recovery Range    
    Serum (n=5) 105 95-109    
    EDTA plasma (n=4) 101 91-105    
                   
                   
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
     
    pg/ml OD1 OD2 Average Corrected    
    500 2.803 2.736 2.770 2.629    
    250 2.362 2.455 2.409 2.268    
    125 1.743 1.862 1.803 1.662    
    62.5 1.123 1.151 1.137 0.996    
    31.25 0.730 0.744 0.737 0.596    
    15.6 0.550 0.537 0.544 0.403    
    7.8 0.306 0.312 0.309 0.168    
    0 0.139 0.143 0.141 　    
  • 数据处理：
  • 货期：
    3-5 working days

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