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  4. Rat collagen type Ⅱ(Col Ⅱ) antibody ELISA kit

Rat collagen type Ⅱ(Col Ⅱ) antibody ELISA kit

  • 中文名称：
    大鼠Ⅱ型胶原(Col Ⅱ)抗体酶联免疫试剂盒
  • 货号：
    CSB-EQ027975RA
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：
    大鼠Ⅱ型胶原(Col Ⅱ)抗体酶联免疫试剂盒（CSB-EQ027975RA）为双抗夹心法ELISA试剂盒，定量检测血清、血浆样本中的Col Ⅱ Ab含量。试剂盒检测范围为0.312 U/mL-20 U/mL，适用于科研领域对骨关节疾病模型的机制研究；适用于评估关节炎动物模型中Col Ⅱ抗体的表达水平，探究软骨退行性病变的分子机制，或用于筛选调节软骨代谢的候选化合物；为软骨生物学、病理学及药物开发研究提供可靠工具本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
  • 别名：
    collagen type 2(Col 2) antibody,_x000D_ collagen type II(Col II) antibody
  • 缩写：
    Col Ⅱ Ab
  • 种属：
    Rattus norvegicus (Rat)
  • 样本类型：
    serum, plasma
  • 检测范围：
    0.312 U/mL-20 U/mL
  • 灵敏度：
    0.078 U/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Signal Transduction
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%        
    Three samples of known concentration were tested twenty times on one plate to assess.    
    Inter-assay Precision (Precision between assays): CV%<10%        
    Three samples of known concentration were tested in twenty assays to assess.      
                   
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat Col Ⅱ antibody in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
    　 Sample Serum(n=4)    
    1:1 Average % 89    
    Range % 86-99    
    1:2 Average % 95    
    Range % 91-100    
    1:4 Average % 93    
    Range % 85-100    
    1:8 Average % 93    
    Range % 82-98    
  • 回收率：
    The recovery of rat Col Ⅱ antibody spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
     
    Sample Type Average % Recovery Range    
    Serum (n=5) 96 91-110    
    EDTA plasma (n=4) 98 89-100    
                   
                   
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
     
    U/ml OD1 OD2 Average Corrected    
    20 2.607 2.725 2.666 2.498    
    10 2.036 2.081 2.059 1.891    
    5 1.328 1.369 1.349 1.181    
    2.5 0.811 0.825 0.818 0.650    
    1.25 0.436 0.421 0.429 0.261    
    0.625 0.349 0.360 0.355 0.187    
    0.312 0.243 0.232 0.238 0.070    
    0 0.169 0.167 0.168 　    
  • 数据处理：
  • 货期：
    3-5 working days

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相关问答

■ 常见问题解答
Q:

I have to use the CSB-EQ027975RA Rat collagen Type II (Col II) antibody kit and I have some questions:
I have to use Culture Medium not Serum or Plasma is this possible?
In other KIt we use before the Standard (CSB-EO8092r) has a concentration of 1000 pg/mL
now this new kit we are going to use has a concentration of 20 U/mL so whic is the relation between Unit and pg?. How can we calculate Collagen II concentration
Thanks in advance for your help

A:
Thanks for your inquiry! CSB-E08092r is for quantitative determination of collagen type Ⅱ(Col Ⅱ) .
CSB-EQ027975RA is for quantitative determination of collagen type Ⅱ(Col Ⅱ) antibody.
U/mL is a quantitative unit and the standard in this kit is represented by U/mL. Sorry there is no converting relationship with pg/mL.
This kit is usually used to test serum and plasma samples. Sorry we didn't test culture medium before and we can not esure 100% if it can be detected out. We suggest you do a pretest first if you want to test it.