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  4. Recombinant Mouse Nuclear receptor subfamily 0 group B member 1 (Nr0b1)

Recombinant Mouse Nuclear receptor subfamily 0 group B member 1 (Nr0b1)

  • 中文名称：
    小鼠Nr0b1重组蛋白
  • 货号：
    CSB-YP726731MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    小鼠Nr0b1重组蛋白
  • 货号：
    CSB-EP726731MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    小鼠Nr0b1重组蛋白
  • 货号：
    CSB-EP726731MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    小鼠Nr0b1重组蛋白
  • 货号：
    CSB-BP726731MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    小鼠Nr0b1重组蛋白
  • 货号：
    CSB-MP726731MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Nr0b1
  • Uniprot No.：
  • 别名：
    Nr0b1; Ahch; Dax1; Nuclear receptor subfamily 0 group B member 1; Nuclear receptor DAX-1
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-472
  • 氨基酸序列
    MAGEDHPWQG SILYNLLMSA KQKHASQEER EVRLGAQCWG CACGAQPVLG GERLSGGQAR SLLYRCCFCG ENHPRQGGIL YSMLTNARQP SVATQAPRAR FGAPCWGCAC GSAEPLVGRE GLPAGQAPSL LYRCCFCGEE HPRQGSILYS LLTSAQQTHV SREAPEAHRR GEWWQLSYCT QSVGGPEGLQ STQAMAFLYR SYVCGEEQPQ QISVASGTPV SADQTPATPQ EQPRAPWWDA SPGVQRLITL KDPQVVCEAA SAGLLKTLRF VKYLPCFQIL PLDQQLVLVR SCWAPLLMLE LAQDHLHFEM MEIPETNTTQ EMLTTRRQET EGPEPAEPQA TEQPQMVSAE AGHLLPAAAV QAIKSFFFKC WSLNIDTKEY AYLKGTVLFN PDLPGLQCVK YIEGLQWRTQ QILTEHIRMM QREYQIRSAE LNSALFLLRF INSDVVTELF FRPIIGAVSM DDMMLEMLCA KL
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Orphan nuclear receptor. Component of a cascade required for the development of the hypothalamic-pituitary-adrenal-gonadal axis. Acts as a coregulatory protein that inhibits the transcriptional activity of other nuclear receptors through heterodimeric interactions. May also have a role in the development of the embryo and in the maintenance of embryonic stem cell pluripotency.
  • 基因功能参考文献：
    1. CpG methylation within the NR0B1 promoter is not involved in the in vivo regulation of NR0B1 expression, whereas the hyperacetylation of histone H4 and the unmethylation of histones H3K9 and H3K27, and their binding to the NR0B1 promoter results in decondensed euchromatin for NR0B1 activation. PMID: 27281610
    2. Sf1 SUMOylation and Dax1 have roles in the physiological cessation of FAdE-mediated Sf1 expression and the resultant regression of the postnatal fetal cortex (X-zone) PMID: 28893949
    3. Findings indicate that the Gata6 promoter is activated by Esrrb in association with Ncoa3, and Dax1 inhibited activities of Esrrb and Ncoa3, resulting maintenance of the undifferentiated status of embryonic stem (ES) cells. PMID: 27601327
    4. Data indicate that nuclear receptor subfamily 0 group B member 1 (Nr0b1) interacted with androgen receptor (AR) in Sertoli cells (SCs). PMID: 27431683
    5. Nr0b1 is a negative regulator of Zscan4c in mouse embryonic stem cells PMID: 25772165
    6. Dax1 and Nanog have roles in stabilizing mouse embryonic stem cells and induced pluripotency PMID: 25284313
    7. Here we review the current knowledge on properties, functions and mechanisms of DAX1 action. PMID: 25916112
    8. Insulin-mediated induction of DAX-1 in Leydig cells of testis may be a key regulatory step of serum sex hormone level in insulin-resistant states. PMID: 23589295
    9. Dax1 functions as a negative regulator of Esrrb and Oct3/4, and these molecules form a regulatory loop for controlling the pluripotency and self-renewal capacity of embryonic stem cells. PMID: 23508100
    10. Transcriptional differences between mouse embryonic and epiblast stem cells is mediated through a Sox2/Esrrb heterodimer regulating Nr0b1. PMID: 23169531
    11. Excess DAX1 leads to XY ovotesticular disorder of sex development (DSD) in mice by inhibiting steroidogenic factor-1 (SF1) activation of the testis enhancer of SRY-box-9 (Sox9). PMID: 22294746
    12. overexpression of DAX-1 had no effect on the retinoic acid-induced differentiation of P19 cells to either endodermal or neuronal cells PMID: 21678401
    13. these data reveal that both Dax1(-/Y) mice and patients with X-linked AHC exhibit adrenal failure that is consistent with adrenocortical subcapsular progenitor cell depletion and argue for a significant role of Dax1 in maintenance of these cells. PMID: 21733829
    14. These data indicate that LRH-1 and Nanog cooperate to regulate Dax1 expression in mouse embryonic stem cells. PMID: 20937355
    15. Dax1 plays an important role in the maintenance of pluripotency in mES cells through interaction with LRH-1 and transcriptional activation of Oct4 and other genes PMID: 20943815
    16. DAX-1 acts as a novel corepressor of liver X receptor alpha and inhibits hepatic lipogenesis PMID: 20080977
    17. describe phenotypic spectrum of disorders associated with mutations and reveal how the discovery of naturally occurring mutations is helping to unravel the role in development and disease [review] PMID: 11738790
    18. important regulators of steroidogeneisis are present in human skin and its appendages. PMID: 11886523
    19. expression is dependent on steroidogenic factor 1 in the developing gonad PMID: 11923472
    20. study revealed that the expression of Dax-1 in the adrenal cortex is regulated by androgen and the receptor PMID: 12081648
    21. the specificities of LXXLL-related motifs in Dax-1 based on their amino acid sequences play an important role in regulation of orphan receptors. PMID: 12482977
    22. Data show that Dax1 plays a crucial role in testis differentiation by regulating the development of peritubular myoid cells and the formation of intact testis cords. PMID: 12538527
    23. Wnt4 signaling mediates the increased expression of Dax-1 as the ovary becomes sexually differentiated PMID: 12554773
    24. testis phenotype of Dax1 knockout mice reflects the combined effects of Dax1 deficiency in both Sertoli and Leydig cells PMID: 12606353
    25. The results reveal that the subcellular localization of Dax-1 is influenced by the presence of Ad4 binding protein/steroidogenic factor 1, and that two regions of Dax-1 have important roles for this process. PMID: 12610109
    26. required for testis differentiation PMID: 12679814
    27. Required for normal steroidogenic axis development. PMID: 14678822
    28. DAX-1 may play a key role in the regulation of Nur77-mediated steroidogenesis in testicular Leydig cells PMID: 15155786
    29. Nuclear receptors Sf1 and Dax1 function cooperatively to mediate somatic cell differentiation during testis development. PMID: 15829514
    30. upregulation of Sox9 expression depends on the coordinated expression of Dax1, Sry and Tda1 PMID: 15944188
    31. The estrogen receptor-related receptor gamma belongs to a new autoregulatory loop that governs DAX-1 gene expression. PMID: 16314306
    32. Nr0b1 functions in a novel role in the maintenance of a relatively undifferentiated state and suggest that the failure of conventional murine Nr0b1 knockout attempts may be due to disregulated differentiation. PMID: 16466956
    33. DAX-1 expression is regulated during mammary epithelial cell differentiation PMID: 16627587
    34. STAT3 and Oct3/4, essential transcription factors for ES cell self-renewal, are involved in the regulation of Dax1 expression. PMID: 18471437
    35. Dax1 deficiency on a partial B6 genetic background results in further modulation of gene expression changes that affect both Sertoli cell and germ cell fate PMID: 18633137
    36. DAX-1 influences the protein kinase signaling pathways involved in the regulation of the StAR protein and steroidogenesis in mouse Leydig tumor cells. PMID: 18787026
    37. These findings reveal novel functions of SRA and Dax-1 in steroidogenesis and adrenal biology. PMID: 19188450
    38. Data suggest that Dax1, a novel molecule interacting with Oct3/4, functions as a negative regulator of Oct3/4 in embryonic stem cells. PMID: 19528230
    39. Data show that Dax-1 is an essential element in the maintenance of ES cell pluripotency and have implications for the understanding of stem cell function in both physiological and clinical settings where Dax-1 plays a pivotal role. PMID: 19530134
    40. DAX-1 inhibits transcriptional activity of HNF4alpha and modulates hepatic gluconeogenic gene expression PMID: 19651776

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  • 亚细胞定位：
    Nucleus. Cytoplasm.
  • 蛋白家族：
    Nuclear hormone receptor family, NR0 subfamily
  • 组织特异性：
    Expressed in adult cerebral cortex, spinal cord, thymus, heart, lung, ovary, testis, adrenal gland, hypothalamus, spleen and kidney.
  • 数据库链接：

    KEGG: mmu:11614

    STRING: 10090.ENSMUSP00000026036

    UniGene: Mm.5180



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