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  4. Recombinant Mouse Properdin (Cfp)

Recombinant Mouse Properdin (Cfp)

  • 中文名称：
    Recombinant Mouse Properdin(Cfp)
  • 货号：
    CSB-YP005291MO
  • 规格：
    ￥2208
  • 图片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
    • Based on the SEQUEST from database of Yeast host and target protein, the LC-MS/MS Analysis result of CSB-YP005291MO could indicate that this peptide derived from Yeast-expressed Mus musculus (Mouse) Cfp.
    • Based on the SEQUEST from database of Yeast host and target protein, the LC-MS/MS Analysis result of CSB-YP005291MO could indicate that this peptide derived from Yeast-expressed Mus musculus (Mouse) Cfp.
  • 其他：

产品详情

  • 纯度：
    Greater than 90% as determined by SDS-PAGE.
  • 基因名：
    Cfp
  • Uniprot No.：
  • 别名：
    Cfp; Pfc; Properdin; Complement factor P
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full Length of Mature Protein
  • 来源：
    Yeast
  • 分子量：
    49.9kDa
  • 表达区域：
    23-464aa
  • 氨基酸序列
    SDPVLCFTQYEESSGRCKGLLGRDIRVEDCCLNAAYAFQEHDGGLCQACRSPQWSAWSLWGPCSVTCSEGSQLRHRRCVGRGGQCSENVAPGTLEWQLQACEDQPCCPEMGGWSEWGPWGPCSVTCSKGTQIRQRVCDNPAPKCGGHCPGEAQQSQACDTQKTCPTHGAWASWGPWSPCSGSCLGGAQEPKETRSRSCSAPAPSHQPPGKPCSGPAYEHKACSGLPPCPVAGGWGPWSPLSPCSVTCGLGQTLEQRTCDHPAPRHGGPFCAGDATRNQMCNKAVPCPVNGEWEAWGKWSDCSRLRMSINCEGTPGQQSRSRSCGGRKFNGKPCAGKLQDIRHCYNIHNCIMKGSWSQWSTWSLCTPPCSPNATRVRQRLCTPLLPKYPPTVSMVEGQGEKNVTFWGTPRPLCEALQGQKLVVEEKRSCLHVPVCKDPEEKKP
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal 6xHis-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
    Note: If you have any special requirement for the glycerol content, please remark when you place the order.
    If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • 产品描述：

    The first step of making this recombinant Mouse Cfp protein is synthesizing the Cfp gene, the synthesized gene is then cloned into an expression vector which is a cDNA plasmid that includes a promoter sequence and an antibiotic-resistant gene. It also has N-terminal 6xHis tag encoding a fusion tag for downstream protein purification or identification. The antibiotic-resistant gene enables the selection of cells carrying the plasmid in antibiotic-based media and then transfects cells with a DNA vector that contains the template and then culturing the cells so that they transcribe and translate the desired protein. The recombinant Cfp protein is purified by ion-exchange chromatography or affinity purification. And the purity is 90%+ by SDS-PAGE.

    Cfp is a protein coding gene that encodes Properdin (Complement factor P). According to some studies, Cfp may have the following features.
    Properdin (Complement factor P) is expressed in choroidal neovascularization in 50%+ of AMD patients. Properdin is a pattern recognition molecule that binds to certain microbial surfaces, apoptotic and necrotic cells. Properdin not only stabilizes but also initiates complement activation. Properdin interacts with immune cells and is involved in many immune responses. Properdin is only expressed in the myeloid cell line HL-60, the monocytic cell line U-937 and the monocytic cell line Mono Mac 6.

  • Datasheet & COA：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    A positive regulator of the alternate pathway of complement. It binds to and stabilizes the C3- and C5-convertase enzyme complexes. Inhibits CFI-CFH mediated degradation of Inhibits CFI-CFH mediated degradation of Complement C3 beta chain (C3b).
  • 基因功能参考文献：
    1. The results show that Properdin plays a key role in allergen-induced airway inflammation and represents a potential therapeutic target for human asthma. PMID: 26116506
    2. s conclude that properdin controls the strength of immune responses by affecting humoral as well as cellular phenotypes during acute bacterial infection and ensuing inflammation. PMID: 24728387
    3. Properdin deficiency exacerbated renal injury in mice lacking complement factor H. PMID: 23184055
    4. deficiency of properdin in mice with factor H mutation exacerbates C3 glomerulonephritis PMID: 23204401
    5. Murine antigonococcal antiserum required functional properdin to kill C4BP-binding strains, but not C4BP-nonbinding strains. PMID: 22368277
    6. properdin may be added to the list of complement proteins which influence lipid metabolism, energy storage and insulin resistance, and further support the hypothesis of a dual role of complement in adipose tissue PMID: 22387270
    7. At the initial phase of local, zymosan-induced inflammation, properdin-deficient and wild-type mice showed bone erosion, proteoglycan loss and cell infiltration. PMID: 20226532
    8. Properdin localizes to mast cells, has the ability to directly associate with Escherichia coli DH5alpha, and plays a significant role in the outcome of polymicrobial sepsis. PMID: 18292556
    9. properdin deficiency attenuates zymosan-induced shock and exacerbates LPS-induced shock. PMID: 18453618

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  • 亚细胞定位：
    Secreted.
  • 数据库链接：

    KEGG: mmu:18636

    STRING: 10090.ENSMUSP00000001156

    UniGene: Mm.3064