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  4. Pig growth hormone,GH ELISA Kit

Pig growth hormone,GH ELISA Kit

  • 中文名称：
    猪生长激素(GH)酶联免疫试剂盒
  • 货号：
    CSB-E06813p
  • 规格：
    96T/48T
  • 价格：
    ￥4200/￥3000
  • 其他：

产品详情

  • 产品描述：
    猪生长激素（GH）酶联免疫试剂盒（货号CSB-E06813p）基于竞争法定量检测猪血清、血浆及组织匀浆样本中的生长激素浓度。GH是由垂体前叶分泌的多肽类激素，在动物生长发育、蛋白质合成及脂肪代谢中起关键调控作用。CUSABIO试剂盒采用预包被抗体的酶标板，通过样本中天然GH与酶标GH竞争结合特异性抗体的原理进行检测，标准曲线覆盖2.5-100 ng/mL线性范围，最低检测限可达0.625 ng/mL，适用于动物生理学、营养代谢研究及生长调控机制探索等科研领域。实验操作全程约需3.5小时，包含样本前处理、孵育及显色步骤，提供标准品、检测抗体、显色液等完整组分。建议检测前离心处理液态样本，组织样本需制备为10%匀浆液，避免反复冻融以保证检测稳定性。该试剂盒可为畜牧养殖优化、饲料效能评估等研究提供可靠数据支持，实验结果需结合分光光度计（450 nm波长）分析。
  • 别名：
    GH1 ELISA Kit; Somatotropin ELISA Kit; Growth hormone ELISA Kit
  • 缩写：
  • Uniprot No.：
  • 种属：
    Sus scrofa (Pig)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    2.5 ng/mL-100 ng/mL
  • 灵敏度：
    2.5 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Signal Transduction
  • 测定原理：
    quantitative
  • 测定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<15%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<15%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of pig GH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:1 Average % 92  
    Range % 87-96  
    1:2 Average % 95  
    Range % 89-100  
    1:4 Average % 94  
    Range % 88-99  
    1:8 Average % 95  
    Range % 90-103  
  • 回收率：
    The recovery of pig GH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 96 90-102  
    EDTA plasma (n=4) 98 92-103  
                 
                 
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average    
    0 1.905 1.834 1.870    
    2.5 1.298 1.282 1.290    
    7.5 1.024 1.004 1.014    
    23 0.739 0.722 0.731    
    50 0.567 0.531 0.549    
    100 0.342 0.338 0.340    
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Plays an important role in growth control. Its major role in stimulating body growth is to stimulate the liver and other tissues to secrete IGF-1. It stimulates both the differentiation and proliferation of myoblasts. It also stimulates amino acid uptake and protein synthesis in muscle and other tissues.
  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    Somatotropin/prolactin family
  • 数据库链接：


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