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  4. Rat growth hormone,GH ELISA KIT

Rat growth hormone,GH ELISA KIT

  • 中文名称：
    大鼠生长激素(GH)酶联免疫试剂盒
  • 货号：
    CSB-E07342r
  • 规格：
    96T/48T
  • 价格：
    ￥3900/￥2500
  • 其他：

产品详情

  • 产品描述：
    CUSABIO定量检测大鼠生长激素（GH）的酶联免疫试剂盒（货号CSB-E07342r），基于双抗体夹心法原理开发，可精确测定血清、血浆、细胞培养上清液及组织匀浆样本中的GH含量。GH是垂体前叶分泌的重要多肽激素，参与调控机体生长、代谢及组织修复等生理过程，在动物生长发育研究、代谢疾病模型建立及内分泌功能评估中具有重要科研价值。试剂盒检测灵敏度高，线性范围覆盖3.12至200 pg/mL，可满足低浓度样本的精准检测需求。实验操作采用预包被特异性抗体的96孔板，通过标准品与样本中GH竞争结合检测抗体，配合显色反应实现定量分析，重复性良好。该试剂盒适用于体外实验中对大鼠模型相关生物样本的GH水平监测，如探究生长调节机制、评估药物干预对激素分泌的影响或研究代谢相关疾病的分子机制等科研领域，为动物实验提供可靠的检测工具。
  • 别名：
    Ghrh ELISA Kit; Somatoliberin ELISA Kit; Growth hormone-releasing factor ELISA Kit; GRF ELISA Kit; Growth hormone-releasing hormone ELISA Kit; GHRH ELISA Kit
  • 缩写：
  • Uniprot No.：
  • 种属：
    Rattus norvegicus (Rat)
  • 样本类型：
    serum, plasma, cell culture supernates, tissue homogenates
  • 检测范围：
    3.12 pg/mL-200 pg/mL
  • 灵敏度：
    0.78 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Signal Transduction
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat GH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:100Average %98
    Range %94-105
    1:200Average %96
    Range %90-102
    1:400Average %100
    Range %94-106
    1:800Average %93
    Range %89-97
  • 回收率：
    The recovery of rat GH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9287-98
    EDTA plasma (n=4)9691-101
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    2002.718 2.801 2.760 2.580
    1002.346 2.405 2.376 2.196
    501.754 1.763 1.759 1.579
    251.126 1.157 1.142 0.962
    12.50.657 0.668 0.663 0.483
    6.250.458 0.467 0.463 0.283
    3.120.341 0.358 0.350 0.170
    00.179 0.181 0.180 　
  • 本试剂盒所含材料：
    • A micro ELISA plate --The 96-well plate has been pre-coated with an anti-rat GH antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
    • Two vials lyophilized standard --Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
    • One vial Biotin-labeled GH antibody (100 x concentrate) (120 μl/bottle) --Act as the detection antibody.
    • One vial HRP-avidin (100 x concentrate) (120 μl/bottle) --Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
    • One vial Biotin-antibody Diluent (15 ml/bottle) --Dilute the high concentration Biotin-antibody to an appropriate working solution.
    • One vial HRP-avidin Diluent (15 ml/bottle) --Dilute the high concentration HRP-avidin solution to an appropriate solution.
    • One vial Sample Diluent (50 ml/bottle)--Dilute the sample to an appropriate concentration.
    • One vial Wash Buffer (25 x concentrate) (20 ml/bottle) --- Wash away unbound or free substances.
    • One vial TMB Substrate (10 ml/bottle) --Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
    • One vial Stop Solution (10 ml/bottle) --Stop the color reaction. The solution color immediately turns from blue to yellow.
    • Four Adhesive Strips (For 96 wells) --Cover the microplate when incubation.
    • An instruction manual

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  • 本试剂盒不含材料：
    • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
    • An incubator can provide stable incubation conditions up to 37°C±5°C.
    • Centrifuge
    • Vortex
    • Squirt bottle, manifold dispenser, or automated microplate washer
    • Absorbent paper for blotting the microtiter plate
    • 50-300ul multi-channel micropipette
    • Pipette tips
    • Single-channel micropipette with different ranges
    • 100ml and 500ml graduated cylinders
    • Deionized or distilled water
    • Timer
    • Test tubes for dilution

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  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    GRF is released by the hypothalamus and acts on the adenohypophyse to stimulate the secretion of growth hormone.
  • 基因功能参考文献：
    1. GHRH agonistic analogs, JI-34 and MR-356 alleviated radiation-induced damage of cardiac myocytes. PMID: 27480202
    2. findings support the hypothesis that antagonizing the signaling of GHRH in T1D may improve GLP-1 function in the small intestine, which, in turn, diminishes TRL and reduces renal and vascular complications PMID: 26831066
    3. JI-38 had shown promising results in multiple fields including cardiology, diabetes and wound healing, our new, more potent, GHRH agonists should manifest additional efficacy for possible medical applications. PMID: 24373935
    4. GHRH protects the heart from ischemia/reperfusion injury through receptor-mediated mechanisms, leading to activation of RISK and SAFE pathways, which converge on mitochondria and possibly on AMP-activated protein kinase. PMID: 23417421
    5. We present evidence that growth retardation in upper airway obstruction is associated with a reduction in hypothalamic GHRH content. PMID: 21406516
    6. Growth hormone expression rises in pituitary gland during normal pregnancy, while its receptor mRNA level in hypothalamus decreases. PMID: 20814072
    7. GABAergic neurons in the median preoptic nucleus and ventrolateral preoptic nucleus are potential targets of the sleep-regulatory actions of GHRH. PMID: 19889861
    8. binding of [125I-Tyr(10)]hGHRH NH(2) to medullary homogenates was specific, time-dependent, reversible and saturable (K(d): 28 nM; B(max): 30 fmol/mg prot.). PMID: 11814616
    9. direct interactions between the growth hormone releasing hormone and growth hormone secretagogue receptors may explain the observed effects of of ghrelin and growth hormone secretagogues on signal transduction PMID: 12446584
    10. Review. Growth hormone-releasing hormone (GHRH) is a hypothalamic hormone that is essential for normal expansion of the somatotrope lineage during pituitary development. PMID: 12511850
    11. Changes in ghrelin and ghrelin receptor expression according to feeding status. PMID: 12876464
    12. Growth hormone releasing hormone (GHRH) is involved in the transcriptional regulation of the GH gene. PMID: 14664705
    13. results suggest that growth hormone secretagogue receptor is involved in the up-regulation of growth hormone releasing hormone and neuropeptide Y expression PMID: 16728494
    14. RFRP-3 infusion elicited a significant increase in growth hormone-releasing hormone mRNA and plasma growth hormone levels compared to control rats. PMID: 18628614
    15. Growth hormone-releasing hormone promotes survival of cardiac myocytes in vitro and protects against ischaemia-reperfusion injury in rat heart. PMID: 19293247
    16. Postsynaptic localization of sst(1) receptors and synaptic connectivity may be an important mechanism by which sst(1) mediates SRIF's cyclical effects on ARC GHRH neurons to generate the ultradian rhythm of GH secretion. PMID: 19553459

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  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    Glucagon family
  • 数据库链接：


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