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  4. Rat N-terminal procollagen Ⅲ propeptide,PⅢNP ELISA Kit

Rat N-terminal procollagen Ⅲ propeptide,PⅢNP ELISA Kit

  • 中文名称：
    大鼠Ⅲ型前胶原氨基末端肽(PⅢNP)酶联免疫试剂盒
  • 货号：
    CSB-E08096r
  • 规格：
    96T/48T
  • 价格：
    ￥3900/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Rat PⅢNP ELISA Kit was designed for the quantitative measurement of Rat PⅢNP protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.625 ng/mL-40 ng/mL and the sensitivity is 0.156 ng/mL.

  • 别名：
    N-terminal procollagen 3 propeptide,P3NP,_x000D_ N-terminal procollagen III propeptide,PIIINP
  • 缩写：
    PⅢNP
  • 种属：
    Rattus norvegicus (Rat)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    0.625 ng/mL-40 ng/mL
  • 灵敏度：
    0.156 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Others
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat PⅢNP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:1 Average % 97  
    Range % 92-101  
    1:2 Average % 98  
    Range % 93-102  
    1:4 Average % 92  
    Range % 87-95  
    1:8 Average % 95  
    Range % 91-98  
  • 回收率：
    The recovery of rat PⅢNP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 91 85-94  
    EDTA plasma (n=4) 98 94-105  
                 
                 
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average Corrected  
    40 2.102 2.077 2.090 1.959  
    20 1.412 1.447 1.430 1.299  
    10 0.924 0.945 0.935 0.804  
    5 0.592 0.608 0.600 0.469  
    2.5 0.360 0.374 0.367 0.236  
    1.25 0.289 0.271 0.280 0.149  
    0.625 0.211 0.222 0.217 0.086  
    0 0.130 0.132 0.131    
  • 数据处理：
  • 货期：
    3-5 working days

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