Rat amyloid beta peptide 1-40,Aβ1-40 ELISA Kit

产品详情

文献引用 (7) 说明书下载

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

CUSABIO大鼠β淀粉样蛋白1-40（Aβ1-40）酶联免疫检测试剂盒（货号：CSB-E08302r），采用双抗体夹心法原理实现精准定量检测，适用于血清、血浆及组织匀浆样本的分析。Aβ1-40作为阿尔茨海默病等神经退行性疾病研究中的关键生物标志物，其异常沉积与神经元损伤密切相关。试剂盒检测灵敏度达0.156 pg/mL，线性范围为0.625-40 pg/mL，仅需100μL样本即可完成检测流程，配套预包被特异性抗体及优化反应体系确保数据可靠性。该产品可广泛应用于神经科学领域的基础研究，例如评估疾病模型中Aβ1-40的病理积累水平、探究药物干预对淀粉样蛋白代谢的影响，或通过体液检测分析个体化模型的生物标志物动态变化。实验操作兼容常规酶标仪检测，提供标准品及生物素化检测抗体，满足科研场景下高灵敏度、高特异性的定量分析需求。
别名：

amyloid beta peptide 1-40,Aβ1-40,amyloid β peptide 1-40
缩写：

Aβ1-40
种属：

Rattus norvegicus (Rat)
样本类型：

serum, plasma, tissue homogenates
检测范围：

0.625 pg/mL-40 pg/mL
灵敏度：

0.156 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Neuroscience
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat Aβ1-40 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	94
	Range %	90-98
1:2	Average %	87
	Range %	82-91
1:4	Average %	90
	Range %	85-94
1:8	Average %	105
	Range %	100-113

回收率：

The recovery of rat Aβ1-40 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	98	94-102
EDTA plasma (n=4)	93	87-98

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.