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  4. Rat Transferrin(TF) ELISA Kit

Rat Transferrin(TF) ELISA Kit

  • 中文名称：
    大鼠转铁蛋白(TF)酶联免疫试剂盒
  • 货号：
    CSB-E12723r
  • 规格：
    96T/48T
  • 价格：
    ￥3800/￥2500
  • 其他：

产品详情

  • 产品描述：
    CUSABIO专为大鼠样本设计的转铁蛋白（TF）定量检测试剂盒（货号：CSB-E12723r），采用双抗体夹心酶联免疫吸附法（Sandwich ELISA），适用于血清、血浆及组织匀浆样本的科研分析。转铁蛋白是脊椎动物体内重要的铁结合糖蛋白，负责铁离子的运输与代谢调控，其水平变化常与机体铁稳态、炎症反应及营养状况密切相关。试剂盒检测范围覆盖7.8至500 pmol/mL的宽动态区间，可精准检测样本中TF的浓度梯度。实验操作基于预包被特异性抗体的微孔板，通过两步孵育结合样本中的目标蛋白，配合酶标二抗及底物显色系统实现定量分析，全程约需4.5小时完成。该试剂盒广泛应用于铁代谢研究、肝脏功能评估、营养干预实验以及疾病动物模型（如贫血、慢性炎症或铁过载相关病理）的机制探索，为体外实验提供可靠的生物标志物检测方案。试剂盒包含预混标准品、检测抗体及即用型底物，支持96孔板规格，批内重复性良好，适用于实验室常规批量检测需求。
  • 别名：
    Tf ELISA Kit; Serotransferrin ELISA Kit; Transferrin ELISA Kit; Beta-1 metal-binding globulin ELISA Kit; Liver regeneration-related protein LRRG03 ELISA Kit; Siderophilin ELISA Kit
  • 缩写：
  • Uniprot No.：
  • 种属：
    Rattus norvegicus (Rat)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    7.8 pmol/mL-500 pmol/mL
  • 灵敏度：
    1.95 pmol/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Cardiovascular
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat TF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:100Average %89
    Range %85-92
    1:200Average %96
    Range %89-104
    1:400Average %90
    Range %84-96
    1:800Average %93
    Range %87-99
  • 回收率：
    The recovery of rat TF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9689-98
    EDTA plasma (n=4)9489-99
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pmol/mlOD1OD2AverageCorrected
    5002.042 2.143 2.093 1.958
    2501.682 1.696 1.689 1.554
    1251.197 1.234 1.216 1.081
    62.50.803 0.819 0.811 0.676
    31.20.392 0.407 0.400 0.265
    15.60.260 0.298 0.279 0.144
    7.80.173 0.180 0.177 0.042
    00.134 0.136 0.135 　
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Transferrins are iron binding transport proteins which can bind two Fe(3+) ions in association with the binding of an anion, usually bicarbonate. It is responsible for the transport of iron from sites of absorption and heme degradation to those of storage and utilization. Serum transferrin may also have a further role in stimulating cell proliferation.
  • 基因功能参考文献：
    1. our data establish that blood TSAT exerts a critical role in experimental stroke-induced brain damage. In addition, our findings suggest that the protective effect of iron-free transferrin (apotransferrin, ATf) at the neuronal level resides in preventing NMDA-induced HTf uptake and ROS production, which in turn reduces neuronal damage PMID: 29248829
    2. In brain interstitial fluid, transferrin-bound iron and non-transferrin-bound iron (NTBI) exist as potential iron sources PMID: 25649872
    3. Transferrin and thyroid hormone converge to regulate oligodendrocyte maturation and myelinogenesis. PMID: 25595122
    4. This evidence supports a key role of Tf on the generation of oligodendrocytes (OLs) from Neural stem and progenitor cells (NSC/NPCs)and highlights its potential in demyelinating disorder treatment. PMID: 22479482
    5. Transferrin is involved in the uptake of the radiopharmaceutical gallium-67 (67Ga) by regenerating liver tissue following partial hepatectomy but is not involved in the entry of 67Ga into hepatocytes. PMID: 11767098
    6. data show that brain cells have the capacity to maintain minimum iron levels during iron deficiency which may be associated with increased iron-Tf uptake, stabilization of TfR mRNA, or increased Tf mRNA translation efficiency in specific cell types PMID: 12608731
    7. effects of apotransferrin occur early in development during a narrow, transient "temporal window" within which oligodendroglia are sensitive to its action. PMID: 12730961
    8. first report describing presence of serotransferrin precursor protein homolog belonging to transferrin family and sharing epitopes with melanotransferrin in adrenal, its induction by ACTH, and sensitivity to ginkgolide B (adrenotransferrin). PMID: 14701678
    9. compared to transferrin, soluble melanotransferrin plays little or no role in Fe supply to the brain and erythropoietic tissue PMID: 15469901
    10. in addition to hemoglobin-bound iron, Tf-bound iron may contribute to intracerebral hemorrhage-induced brain injury and thrombin may contribute to the latter by facilitating cellular iron uptake PMID: 15637325
    11. Our studies suggest that AEC I is not just a simple barrier for gas exchange, but a functional cell that protects alveolar epithelium from injury. PMID: 16497717
    12. Plays role in maturation of Schwann cells(SC) in physiological conditions as well as in role in facilitating clearance of myelin debris and subsequent nerve regeneration and/or in SC phenotypic change, which led to survival after peripheral nerve injury. PMID: 17628542
    13. Apotransferrin added to oligodendroglial cell cultures can correct the effects of iron deficiency. PMID: 18459135
    14. serotransferrin levels were increased in diestrous as compared with proestrous, estrous, metestrous PMID: 18511206
    15. we have discovered a novel mitochondrial iron transport system that goes awry in Parkinson's disease, and which may provide a new target for therapeutic intervention. PMID: 19250966
    16. synthesis and secretion of transferrin in rat parotid acinar cells PMID: 19464997

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  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    Transferrin family
  • 组织特异性：
    Expressed by the liver and secreted in plasma.
  • 数据库链接：